Biofilm formation by phytopathogenic bacteria Acidovorax citrulli subsp. citrulli and Ralstonia solanacearum

Malafaia, Carolina Barbosa; Barros, Muriel Primon de; Macêdo, Alexandre José; Guerra, Myrzânia de Lira; Souza, Elineide Barbosa de; Correia, Maria Tereza dos Santos; Silva, Márcia Vanusa da

doi:10.24221/jeap.3.4.2018.2018.347-355

Cited by 7 publications

(6 citation statements)

References 42 publications

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“… 62 . Biofilm formation is essential for the virulence of phytopathogenic strains of Acidovorax species 24 , 25 , 27 , 63 – 65 . Our results indicate that LMJ forms biofilm ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

et al. 2020

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Background: Chlamydomonas reinhardtii, a green micro-alga can be grown at the lab heterotrophically or photo-heterotrophically in Tris-Phosphate-Acetate (TAP) medium which contains acetate as the sole carbon source. When grown in TAP medium, Chlamydomonas can utilize the exogenous acetate in the medium for gluconeogenesis using the glyoxylate cycle, which is also present in many bacteria and higher plants. A novel bacterial strain, LMJ, was isolated from a contaminated TAP medium plate of Chlamydomonas. We present our work on the isolation and physiological and biochemical characterizations of LMJ. Methods: Several microbiological tests were conducted to characterize LMJ, including its sensitivity to four antibiotics. We amplified and sequenced partially the 16S rRNA gene of LMJ. We tested if LMJ can utilize cyclic alkanes, aromatic hydrocarbons, poly-hydroxyalkanoates, and fresh and combusted car motor oil as the sole carbon source on Tris-Phosphate (TP) agar medium plates for growth. Results: LMJ is a gram-negative rod, oxidase-positive, mesophilic, non-enteric, pigmented, salt-sensitive bacterium. LMJ can ferment glucose, is starch hydrolysis-negative, and is very sensitive to penicillin and chloramphenicol. Preliminary spectrophotometric analyses indicate LMJ produces pyomelanin. NCBI-BLAST analyses of the partial 16S rRNA gene sequence of LMJ showed that it matched to that of an uncultured bacterium clone LIB091_C05_1243. The nearest genus relative of LMJ is an Acidovorax sp. strain. LMJ was able to use alkane hydrocarbons, fresh and combusted car motor oil, poly-hydroxybutyrate, phenanthrene, naphthalene, benzoic acid and phenyl acetate as the sole carbon source for growth on TP-agar medium plates. Conclusions: LMJ has 99.14% sequence identity with the Acidovorax sp. strain A16OP12 whose genome has not been sequenced yet. LMJ’s ability to use chemicals that are common environmental pollutants makes it a promising candidate for further investigation for its use in bioremediation and, provides us with an incentive to sequence its genome.

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Section: Discussionmentioning

confidence: 99%

“…62 . Biofilm formation is essential for the virulence of phytopathogenic strains of Acidovorax species 24,25,27, [63][64][65] .…”

Section: Pseudomonas Facilis Pseudomonas Delafieldii E Falsen (Ef)mentioning

confidence: 99%

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

et al. 2020

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“…These observations are not fully consistent with the adhesion pattern observed for other plant pathogenic bacteria to their host plant cells. For example, R. solanacearum (Malafaia et al , ) and X. fastidiosa (Tyson et al , ) were shown to bind the walls of the xylem vessels by their cell poles. These observations suggest that the mechanisms used by bacteria to establish physical contact with their host tissue vary either according to the bacterial species, the biophysical properties of the host surface, or both.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the movement of E. psidii in stem tissue was monitored by PCR, and then E. psidii colonization and biofilm formation was investigated in different eucalypt tissues by scanning electron microscopy (SEM). This is a well‐established technique to observe ultrastructural details of bacterial adhesion to surfaces, production of extracellular material and biofilm architecture (El Abed et al , ) that has already been used to study biofilms formed by several phytopathogenic bacteria (Tyson et al , ; Koczan et al , ; Malafaia et al , ). Biofilm formation on polystyrene and borosilicate surfaces using different culture media was also investigated.…”

Section: Introductionmentioning

confidence: 99%

Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

et al. 2020

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Dieback and wilt caused by Erwinia psidii is an emerging disease that has been causing considerable damage in eucalypt plantations. Because it is a recently emerged disease, several aspects of the bacterial interaction with its host still remain to be elucidated. In this work, we studied the E. psidii colonization and biofilm formation in eucalypt tissues by specific detection using PCR and scanning electron microscopy (SEM). The results indicate that the bacterium is able to translocate in stem tissue mainly acropetally, although movement in the basipetal direction was also observed to a lesser extent, always through the xylem. No colonization of phloem tissues was observed. In addition to colonizing the xylem, E. psidii colonized the parenchymatous tissue. The bacterium formed cell aggregates enveloped by fibrillar material that evolved into complex, well‐structured biofilms in stem and leaf tissues. In contrast, no biofilm formation was observed on abiotic surfaces. These observations suggest that biofilm formation plays an important role in the elicitation of dieback and wilt symptoms caused by E. psidii on eucalypt plants. This study not only shows ultrastructural aspects of the E. psidii communities but also tissue damage in eucalypt plants that was associated with the presence of bacterial aggregates and formation of tyloses.

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“…The disease was first reported in Georgia, USA, in 1965 and has been reported in many countries [ 1 ]. Acidovorax citrulli uses a variety of virulence factors, such as the Type III secretion system (T3SS), the Type VI secretion system (T6SS), Type IV pili (T4P), polar flagella, quorum sensing, and biofilms [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ] to induce disease. Acidovorax citrulli strains can be divided into two major groups (I and II) [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

ntrC Contributes to Nitrogen Utilization, Stress Tolerance, and Virulence in Acidovorax citrulli

et al. 2023

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Bacterial fruit blotch (BFB), caused by Acidovorax citrulli, severely damages watermelon, melon, and other cucurbit crops worldwide. Nitrogen, one of the most important limiting elements in the environment, is necessary for the growth and reproduction of bacteria. As a nitrogen-regulating gene, ntrC plays an important role in maintaining bacterial nitrogen utilization and biological nitrogen fixation. However, the role of ntrC has not been determined for A. citrulli. In this study, we constructed a ntrC deletion mutant and a corresponding complementary strain in the background of the A. citrulli wild-type strain, Aac5. Through phenotype assays and qRT-PCR analysis, we investigated the role of ntrC in A. citrulli in nitrogen utilization, stress tolerance, and virulence against watermelon seedlings. Our results showed that the A. citrulli Aac5 ntrC deletion mutant lost the ability to utilize nitrate. The ntrC mutant strain also exhibited significantly decreased virulence, in vitro growth, in vivo colonization ability, swimming motility, and twitching motility. In contrast, it displayed significantly enhanced biofilm formation and tolerance to stress induced by oxygen, high salt, and copper ions. The qRT-PCR results showed that the nitrate utilization gene nasS; the Type III secretion system-related genes hrpE, hrpX, and hrcJ; and the pili-related gene pilA were significantly downregulated in the ntrC deletion mutant. The nitrate utilization gene nasT, and the flagellum-related genes flhD, flhC, fliA, and fliC were significantly upregulated in the ntrC deletion mutant. The expression levels of ntrC gene in the MMX-q and XVM2 media were significantly higher than in the KB medium. These results suggest that the ntrC gene plays a pivotal role in the nitrogen utilization, stress tolerance, and virulence of A. citrulli.

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Biofilm formation by phytopathogenic bacteria Acidovorax citrulli subsp. citrulli and Ralstonia solanacearum

Abstract: Malafaia, C.B.; Barros, M.P.; Macedo, A.J.; Guerra, M. L.; Souza, E.B.; Correia, M.T.S.; Silva, M.V. 347

Cited by 7 publications

References 42 publications

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants

ntrC Contributes to Nitrogen Utilization, Stress Tolerance, and Virulence in Acidovorax citrulli

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