Biofilm formation in erythromycin-resistant Staphylococcus aureus and the relationship with antimicrobial susceptibility and molecular characteristics

Sun, Xinghuai; Wang, Qisheng; Hu, Xiaoxiong; Yao, Wei; Bai, Bing; Wang, Hongyan; Li, Duoyun; Chen, Zhong; Cheng, Hang; Pan, Weiguang; Deng, Minggui; Xu, Guangjian; Tu, Haijun; Chen, Junwen; Deng, Qi; Yu, Zhijian; Zheng, Jinxin

doi:10.1016/j.micpath.2018.08.021

Cited by 28 publications

(23 citation statements)

References 25 publications

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“…Furthermore, although no significant difference was seen, resistance to the antibiotics was higher in the non-strong biofilm producers. This was in agreement with the study of Zhang et al [ 4 ]; who found the high prevalence of resistance to ciprofloxacin, gentamicin, tetracycline, clindamycin, and trimethoprim-sulfamethoxazole in moderate and weak biofilm producers, but in contrast to other studies showing the high resistance in strong biofilm producing S. aureus [ 35 , 36 ]. These results may indicate the importance of drug resistance and some virulence genes such as tst and sea in the pathogenesis of non-strong biofilm producers.…”

Section: Discussionsupporting

confidence: 93%

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

2021

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Background and objective Carriage of virulence factors confers some evolutionary benefit to bacteria, which favors the resistant strains. We aimed to analyze whether antibiotic susceptibility of Staphylococcus aureus strains is affected by agr typing, biofilm formation ability, and virulence profiles. Methods A total of 123 S. aureus clinical isolates were subjected to antimicrobial susceptibility testing by disk diffusion method, biofilm formation by microtiter plate method, as well as polymerase chain reaction screening to identify virulence genes and the accessory gene regulator (agr) types I-IV. A P value < 0.05 was considered significant. Results The most prevalent virulence gene was staphyloxanthin crtN, followed by hemolysin genes, capsular cap8H, toxic shock toxin tst, and enterotoxin sea, respectively. Resistant isolates were more commonly found in the agr-negative group than in the agr-positive group. Isolates of agr type III were more virulent than agr I isolates. Strong biofilm producers showed more antibiotic susceptibility and carried more virulence genes than non-strong biofilm producers. Associations were found between the presence of virulence genes and susceptibility to antibiotics. Carriage of the virulence genes and agr was higher in the inpatients; while, resistance and strong biofilms were more prevalent in the outpatients. Conclusion These findings indicated the presence of several virulence factors, biofilm production capacity, agr types and resistance to antibiotics in clinical S. aureus isolates. Considering the importance of S. aureus for human medicine, an understanding of virulence and resistance relationships would help to reduce the impact of S. aureus infections.

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Section: Discussionsupporting

confidence: 93%

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

2021

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“…Nevertheless, despite the production of substantial biofilm these susceptible ST59 strains have not been observed to disseminate in adult wards where fluoroquinolones are supposed to be in use. Moreover, the production of biofilm has also been shown to be influenced by not just the type of the pathogen but also by resistance to macrolides (Sun et al, 2018), previous exposure to antibiotics and some features of the infected patient (Luther et al, 2018). Baldan et al (2015) suggested that ST22 HA-MRSA could replace ST228 strains because of a higher rate of hemolysin production.…”

Section: The Virulence Component In Mrsamentioning

confidence: 99%

Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents

2020

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“…After drying, 33% glacial acetic acid was added to the well to release the biofilms into the solution, and the optical density at 600 nm (OD600) was measured. 16…”

Section: Biofilm Formationmentioning

confidence: 99%

Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence

Zhan¹,

Xu²,

Zhan³

et al. 2021

IDR

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Introduction: The ability of Staphylococcus aureus to form biofilms is associated with high mortality and treatment costs. Established biofilms cannot be eradicated by many conventional antibiotics due to the development of antibiotic tolerance by S. aureus. Here we report the synthesis and biological characterization of novel small-molecule compounds with antibiofilm activity. Chromone 5-maleimide substitution compounds (CM3a) showed favorable antibacterial activity against S. aureus. Methods: CM3A with antibacterial activity was synthesized and screened. The minimum inhibitory concentration (MIC) of CM3a were determined by the broth microdilution method. Biofilm eradication assay and colony count methods were used to investigate the effect of CM3a on S. aureus biofilm disruption and killing. Changes in biofilm architecture when subjected to CM3a, were visualized using confocal laser scanning microscopy (CLSM). CCK-8 assay and survival rate of Galleria mellonella larvae were used to test the toxicity of CM3a. Results: The minimum inhibitory concentration (MIC) of CM3a against S. aureus was about 26.4 μM. Biofilm staining and laser scanning confocal microscopy analysis showed that CM3a eradicated S. aureus biofilms by reducing the viability of the constituent bacterial cells. On the other hand, CM3a showed negligible toxicity against mouse alveolar epithelial cells and Galleria mellonella larvae. Conclusion: Chromone derivatives CM3a has therapeutic potential as a safe and effective compound for the treatment of S. aureus infection.

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Biofilm formation in erythromycin-resistant Staphylococcus aureus and the relationship with antimicrobial susceptibility and molecular characteristics

Cited by 28 publications

References 25 publications

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents

Chromone Derivatives CM3a Potently Eradicate Staphylococcus aureus Biofilms by Inhibiting Cell Adherence

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