Biofilm formation on polypropylene during start‐up of anaerobic fixed‐bed reactors

Zellner, Gerhard; Diekmann, H.; Austermann-Haun, Ute; Baumgarten, G.; Seyfried, Carl‐Franz

doi:10.1080/08927019309386236

Cited by 14 publications

(4 citation statements)

References 71 publications

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“…This competition will not only be influenced by the availability of these substrates in the reactor, but will also be dependent on other variables such as reactor temperature and pH. In some studies Methanobacteriales such as Methanobacterium and Methanobrevibacter have been reported to be the dominant hydrogen‐ and formate‐consuming methanogens [16–18, 30], while other studies have shown that Methanomicrobiales can also be present in the sludge in high numbers [38] and can even be more abundant than Methanobacteriales [29, 39].…”

Section: Resultsmentioning

confidence: 99%

Characterization of the sulfate-reducing and syntrophic population in granular sludge from a full-scale anaerobic reactor treating papermill wastewater

Elferink¹,

Vorstman²,

Sopjes³

et al. 1998

FEMS Microbiology Ecology

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To gain more insight into the competitive and syntrophic interactions between sulfate-reducing and methanogenic consortia in sulfate-limited anaerobic reactors, the microbial population in granular sludge from a full-scale upflow anaerobic sludge blanket (UASB) reactor was studied, using the 16S rRNA dot-blot hybridization method in combination with most probable number estimates. The wastewater which was treated in the UASB reactor contained mainly starch, acetate, propionate, butyrate and formate, and had a chemical oxygen demand/sulfate ratio of 9.5. Evidence was obtained that acetate was mainly degraded by Methanosaeta-like microorganisms, while propionate was the preferred substrate for sulfate reduction. The Desulfobulbus-like propionate-degrading sulfate reducers in the sludge competed with Syntrophobacter-like bacteria for the available propionate. Hydrogen and formate were probably mainly degraded via methanogenesis by members of the order Methanobacteriales. Hydrogen, formate and butyrate-degrading sulfate reducers could not be characterized with the 16S rRNA probes available to date. The same was true for syntrophic butyrate degraders. z

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Section: Resultsmentioning

confidence: 99%

Characterization of the sulfate-reducing and syntrophic population in granular sludge from a full-scale anaerobic reactor treating papermill wastewater

Elferink¹,

Vorstman²,

Sopjes³

et al. 1998

FEMS Microbiology Ecology

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“…After the repair the previous performance resumed and reached 54.0 g COD l −1 day −1 (94% COD reduction, 2.9 d HRT), and samples L2 and L3 were taken on day 67. The biofilm biomass (as protein with the Folin phenol reagent) and the reducing sugar content of R3 were determined as described [26].…”

Section: Methodsmentioning

confidence: 99%

A study of three anaerobic methanogenic bioreactors reveals that syntrophs are diverse and different from reference organisms

Zellner

Macario

2006

FEMS Microbiology Ecology

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Calibrated antibody probes for homoacetogenic, syntrophic acetogenic, and sulfate‐reducing Bacteria were generated and used, along with probes for methanogenic Archaea, to elucidate the bacterial biofilm population of three fluidized bed bioreactors fed with different substrates. The new probes were for Clostridium formicoaceticum, Syntrophomonas wolfei subsp. wolfei, Syntrophospora bryantii, Syntrophococcus sucromutans strain S195, and Desulfobulbus propionicus strain 1pr3. Microscopic and electron microscopic analyses showed a complex microbial flora in the three bioreactors. This was confirmed by the antigenic fingerprinting method which showed a diversity of methanogens and syntrophs. The subpopulations that could be identified were only moderately or weakly related to reference immunotypes, and were quantitatively small. Thus, these and the major populations from the quantitative and functional standpoints, belong to as yet uncharacterized immunotypes, and possibly include new strains and species.

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“…and hydrogenotrophic and acetotrophic methanogens [176]. Zellner et al [177] reported that in a fixedbed reactor fed with synthetic wastewater containing acetate, propionate and butyrate, oxidation of propionate and butyrate was carried out in part by Desulfobulbus sp. acting as a facultative syntrophic bacterium, and by the obligately syntrophic bacterium, Syntrophospora sp., respectively.…”

Section: Fermentative and Acetogenic Conversions By Sulfate Reducersmentioning

confidence: 99%

Sulfate reduction in methanogenic bioreactors

J.W.H.

Elferink

Visser

et al. 1994

FEMS Microbiology Reviews

245

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In the anaerobic treatment of sulfate‐containing wastewater, sulfate reduction interferes with methanogenesis. Both mutualistic and competitive interactions between sulfate‐reducing bacteria and methanogenic bacteria have been observed. Sulfate reducers will compete with methanogens for the common substrates hydrogen, formate and acetate. In general, sulfate reducers have better growth kinetic properties than methanogens, but additional factors which may be of importance in the competition are adherence properties, mixed substrate utilization, affinity for sulfate of sulfate reducers, relative numbers of bacteria, and reactor conditions such as pH, temperature and sulfide concentration. Sulfate reducers also compete with syntrophic methanogenic consortia involved in the degradation of substrates like propionate and butyrate. In the absence of sulfate these methanogenic consortia are very important, but in the presence of sulfate they are thought to be easily outcompeted by sulfate reducers. However, at relatively low sulfate concentrations, syntrophic degradation of propionate and butyrate coupled to HZ removal via sulfate reduction rather than via methanogenesis may become important. A remarkable feature of some sulfate reducers is their ability to grow fermentatively or to grow in syntrophic association with methanogens in the absence of sulfate.

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Biofilm formation on polypropylene during start‐up of anaerobic fixed‐bed reactors

Cited by 14 publications

References 71 publications

Characterization of the sulfate-reducing and syntrophic population in granular sludge from a full-scale anaerobic reactor treating papermill wastewater

Characterization of the sulfate-reducing and syntrophic population in granular sludge from a full-scale anaerobic reactor treating papermill wastewater

A study of three anaerobic methanogenic bioreactors reveals that syntrophs are diverse and different from reference organisms

Sulfate reduction in methanogenic bioreactors

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