Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage

Jotić, Ana; Božić, Dragana; Milovanović, Jovica; Pavlović, Bojan; Jesić, S; Pelemiš, Mijomir; Novaković, Marko; Ćirković, Ivana

doi:10.1007/s00405-015-3607-8

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…The majority of MRSA isolates in our study represented CC5, typically referred to as hospital-associated strains, and CC8, historically of community origin. In the multivariate analyses, there was no association between the clonal complex of the isolate and biofilm formation, which has been found in other studies (12)(13)(14). This may be due to the limited number of isolates or the clinical source of the isolates used, which may play a role in their biofilm formation.…”

Section: Discussionmentioning

confidence: 47%

Clinical and Genetic Risk Factors for Biofilm-Forming Staphylococcus aureus

Luther

Parente

Caffrey

et al. 2018

Antimicrob Agents Chemother

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The molecular and clinical factors associated with biofilm-forming methicillin-resistant (MRSA) are incompletely understood. Biofilm production for 182 MRSA isolates obtained from clinical culture sites (2004 to 2013) was quantified. Microbiological toxins, pigmentation, and genotypes were evaluated, and patient demographics were collected. Logistic regression was used to quantify the effect of strong biofilm production (versus weak biofilm production) on clinical outcomes and independent predictors of a strong biofilm. Of the isolates evaluated, 25.8% (47/182) produced strong biofilms and 40.7% (74/182) produced weak biofilms. Strong biofilm-producing isolates were more likely to be from multilocus sequence typing (MLST) clonal complex 8 (CC8) (34.0% versus 14.9%; = 0.01) but less likely to be from MLST CC5 (48.9% versus 73.0%; = 0.007). Predictors for strong biofilms were type t008 (adjusted odds ratio [aOR], 4.54; 95% confidence interval [CI], 1.21 to 17.1) and receipt of chemotherapy or immunosuppressants in the previous 90 days (aOR, 33.6; 95% CI, 1.68 to 673). Conversely, patients with high serum creatinine concentrations (aOR, 0.33; 95% CI, 0.15 to 0.72) or who previously received vancomycin (aOR, 0.03; 95% CI, 0.002 to 0.39) were less likely to harbor strong biofilm-producing MRSA. Beta-toxin-producing isolates (aOR, 0.31; 95% CI, 0.11 to 0.89) and isolates with type t895 (aOR, 0.02 95% CI,<0.001 to 0.47) were less likely to produce strong biofilms. Patient outcomes also varied between the two groups. Specifically, patients with strong biofilm-forming MRSA were significantly more likely to be readmitted within 90 days (aOR, 5.43; 95% CI, 1.69 to 17.4) but tended to have decreased 90-day mortality (aOR, 0.36; 95% CI, 0.12 to 1.06). Patients that harbored t008 and received immunosuppressants were more likely to have strong biofilm-producing MRSA isolates. Clinically, patients with strong biofilm-forming MRSA were less likely to die at 90 days but five times more likely to be readmitted.

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Section: Discussionmentioning

confidence: 47%

Clinical and Genetic Risk Factors for Biofilm-Forming Staphylococcus aureus

Luther

Parente

Caffrey

et al. 2018

Antimicrob Agents Chemother

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“…The emergence of MRSA and quinolone-resistant P aeruginosa has created a serious therapeutic problem for post-tympanostomy tube otorrhoea 11 , 12 . Surgical removal of the infected tubes is an effective treatment for biofilm on tympanostomy tubes, but it is associated with antibiotic-resistant infections such as MRSA and quinolone-resistant P aeruginosa 13 .…”

Section: Discussionmentioning

confidence: 99%

Comparative efficacies of topical antiseptic eardrops against biofilms from methicillin-resistant Staphylococcus aureus and quinolone-resistant Pseudomonas aeruginosa

Yao

et al. 2018

J. Laryngol. Otol.

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“…Several studies have evaluated the BF ability of common foodborne pathogens. Jotic et al (2016) assessed the BF of 30 isolated Staphylococcus and found that the BF ability between different strains was different. Lianou and Koutsoumanis (2012) found that the BF ability of Salmonella had no correlation with the serotype and strain type of the bacteria, which might be related to the different experimental strains in different studies.…”

Section: Discussionmentioning

confidence: 99%

Effect of lactic acid stress on biofilm formation of Escherichia coli O26 at different temperatures

et al. 2020

Journal of Food Safety

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Similar to Escherichia coli O157, non‐O157 also inherits certain acid resistance, and its stress response can be induced under acidic conditions, which may be affected by temperature. In this study, O26, the main serotype of non‐O157 E. coli, was investigated in order to evaluate the effect of lactic acid stress on its biofilm formation (BF) at different temperatures. Firstly, the BF ability of 22 isolates of E. coli O26 were compared by means of crystal violet staining and the result showed that all strains had certain BF ability with variation among strains. Then, the strain 126Z with strong capacity and strain G13Z2 with medium capacity were selected for BF experiment in acidic TSB (pH 4.0 and 5.0, adjusted with lactic acid) at 15°C and 25°C, respectively. The results showed that the BF of O26 was decreased with increasing concentration of lactic acid, and the higher the concentration of lactic acid was, the less the amount of biofilm formed. Moreover, the effect of temperature on BF of different capacity strains was related to the pH value, that is, with a lower concentration of lactic acid, the higher the temperature was, the faster the biofilm amount increased. Under a higher acid concentration, the BF of two strains showed different dynamic changes and were inhibited in the later stage, in which the biofilm amount of 126Z decreased significantly at 15°C, while that of G13Z2 decreased more significant at 25°C. This observation was further confirmed by scanning electron microscope (SEM) and confocal laser scanning microscopy (CLSM). This finding suggests that combined temperature and lactic acid intervention may be an effective method to prevent BF, and the results of present work would provide scientific basis for the risk assessment of E. coli O26 in food processing.

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Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage

Cited by 7 publications

References 28 publications

Clinical and Genetic Risk Factors for Biofilm-Forming Staphylococcus aureus

Clinical and Genetic Risk Factors for Biofilm-Forming Staphylococcus aureus

Comparative efficacies of topical antiseptic eardrops against biofilms from methicillin-resistant Staphylococcus aureus and quinolone-resistant Pseudomonas aeruginosa

Effect of lactic acid stress on biofilm formation of Escherichia coli O26 at different temperatures

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