Biofilm-Forming Capacity ofStaphylococcus epidermidis,Staphylococcus aureus, andPseudomonas aeruginosafrom Ocular Infections

Hou, Wenbo; Sun, Xing‐Guo; Wang, Zhi­qun; Zhang, Yang

doi:10.1167/iovs.11-9114

Cited by 79 publications

(69 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Phenotypically, quantification of biofilm formation under static conditions was determined by microtiter assay as already reported with little modification (11). Briefly, 18 h LB-grown cultures diluted 1:100 in fresh LB containing 0.5 glucose were inoculated (200 L per well) into tissue culture-treated 96-well polystyrene microtiter plates (Costar, Corning, NY, USA).…”

Section: Discussionmentioning

confidence: 99%

Predation Efficacy of Bdellovibrio bacteriovorus on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Sun

Hou

et al. 2017

Jpn J Infect Dis

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Predation Efficacy of Bdellovibrio bacteriovorus on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Sun

Hou

et al. 2017

Jpn J Infect Dis

View full text Add to dashboard Cite

“…In the study of Semczuk, all the isolates producing biofilm phenotypically harbored icaAD genes. In Hou's study, among 55.56% of isolates that produced biofilm in phenotypic test, 11.11% contained the icaA gene (26), but the other genes has not been investigated. In this study, methicillin resistant isolates harbored higher rate of icaADBC genes compared to MSSA, but no significant difference was confirmed, similar to two other studies (27,28).…”

Section: Discussionmentioning

confidence: 99%

Several Virulence Factors of Multidrug-Resistant Staphylococcus aureus Isolates From Hospitalized Patients in Tehran

Ghasemian¹,

Peerayeh²,

Bakhshi³

et al. 2015

Int J Enteric Pathog

View full text Add to dashboard Cite

Background: Biofilm formation plays an important role in resistance of Staphylococcus aureus isolates; especially multidrug-resistant isolates are a threat to healthcare settings. Objectives: The aims of this study were to detect biofilm formation and presence of several related genes among multidrug-resistant (MDR) isolates of Staphylococcus aureus. Patients and Methods: A total Of 209 S. aureus strains were isolated from patients and identified by conventional diagnostic tests. The multidrug-resistant MRSA isolates were detected by antibiotic susceptibility test. The phenotypic biofilm formation was detected by microtitre tissue plate assay. The polymerase chain reaction (PCR) was performed to detect the mecA, Staphylococcal Cassette Chromosome mec (SCCmec) types, accessory gene regulatory (agr) genes, the icaADBC and several genes encoding staphylococcal surface proteins including clfAB, fnbAB, fib, eno, can, ebps and bbp genes with specific primers. Results: Sixty-four (30.6%) isolates were methicillin-resistant, among which thirty-six (56.2%) were MDR. These isolates were resistant to amoxicillin, tetracycline, ciprofloxacin, gentamicin, erythromycin and trimethoprim-sulfamethoxazole (except to 6 isolates). All the isolates were susceptible to vancomycin and linezolid. All the MDR-MRSA harbored SCCmec type III. All the MDR-MRSA isolates were strong biofilm producers in the phenotypic test. The majority of MDR-MRSA was belonged to agrI (67%, n = 24), followed by agr II (17%, n = 6), agrIV (11%, n = 4) and agrIII (5.5%, n = 2). The frequency of icaADBC genes were 75% (n = 27), 61% (n = 22), 72% (n = 26) and 72% (n = 26), respectively. Furthermore, the prevalence of clfA, clfB, fnbA, fnbB, fib, can, eno, ebps and bbp genes was 100%, 100%, 67%, 56%, 80%, 63%, 78%, 7% and 0%, respectively. Furthermore, approximately all the MRSA was strong biofilm producers. Conclusions: Multidrug-resistant isolates produced biofilm strongly and the majority harbored most of biofilm related genes, suggesting that biofilm formation is associated to the presence of these genes, and also biofilm production can increase the antibiotic resistance as have demonstrated in MDR-MRSA isolates.

show abstract

“…Several studies indicated the role of icaA and icaD genes in biofilm production and several reported that all of isolates were icaA positive (29). In the study by Hou et al (30), among 55.56% of isolates that produced biofilm phenotypically, 11.11% had icaA gene, but other genes were not investigated. In this study, methicillin resistant isolates harbored higher rate of icaADBC genes, similar to studies conducted by Khan et al (31) and O'neill et al (32).…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

Ghasemian

Peerayeh

Bakhshi

et al. 2015

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

Background:The icaABCD genes encode a Polysaccharide Intercellular Adhesion (PIA), which is a tight structure protecting Staphylococcus aureus community against adverse environmental conditions. The ica dependent biofilm formation plays an important role in persistent infections in hospitalized patients. Objectives: The aim of this study was to detect icaADBC genes encoding PIA among S. aureus isolates from children in Loghman Hospital of Tehran. Materials and Methods: We collected 22 clinical specimens from hospitalized pediatrics and identified the isolates. Then, we detected mecA gene among Methicillin Resistant S. aureus (MRSA), SCCmec types and icaABCD gens by PCR assay and specific primers. Results: Five isolates (22.7%) were methicillin resistant (MRSA) and mecA gene was detected among them. All the MRSA isolates harbored SCCmec type III. Prevalence of icaA, icaB, icaC and icaD in the isolates were 16 (73%), 14 (63.6%), 16 (73%) and 16 (73%), respectively. Moreover, all the MRSA strains were icaADBC positive. Conclusions: Prevalence of icaADBC genes was relatively high among children and also all the four ica genes were detected among MRSA strains.

show abstract

Biofilm-Forming Capacity ofStaphylococcus epidermidis,Staphylococcus aureus, andPseudomonas aeruginosafrom Ocular Infections

Cited by 79 publications

References 33 publications

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Predation Efficacy of <i>Bdellovibrio bacteriovorus</i> on Multidrug-Resistant Clinical Pathogens and Their Corresponding Biofilms

Several Virulence Factors of Multidrug-Resistant Staphylococcus aureus Isolates From Hospitalized Patients in Tehran

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

Contact Info

Product

Resources

About