Biofilm morphology in porous media, a study with microscopic and image techniques

Paulsen, J.E.; Oppen, Eirik; Bakke, Rune

doi:10.1016/s0273-1223(97)00317-x

Cited by 27 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The filamentous growth that we observed is only one possible realization of growth in porous media. Generaliza tions to other growth patterns commonly observed ( , ) cannot be made from this one observation, but some generalizations about filamentous clogging are possible. With the filamentous fungal growth here observed, the more numerous and closer to each other downgradient colonies became intermingled and eventually spanned across the entire SPIE.…”

Section: Discussionmentioning

confidence: 91%

“…Two-dimensional physi cal simulators of porous media etched in glass or silicon have been used over the past decade by petroleum engineers to allow real time microscopic or mesoscopic observations of two- and three-phase flow. Such models can be of a regular ( , ) or random ( , ) pattern. Network models can simulate many aspects of heterogeneous porous media () and allow numerical simulation of flow patterns.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mesoscale and Microscale Observations of Biological Growth in a Silicon Pore Imaging Element

Dupin

McCarty

1999

Environ. Sci. Technol.

View full text Add to dashboard Cite

Better physical understanding is needed of the processes affecting biological growth in aquifers, filtration beds, and recharge basins. Toward this end, a two-dimensional random width network pore model etched in a silicon wafer was developed to simulate microbial growth in porous media representative of fine sand. This Silicon Pore Imaging Element (SPIE) was seeded with a mixed culture and fed with 0.34 mM acetate under aerobic conditions and at fixed flow rate. Twelve filamentous colonies grew in a dense manner in the upgradient and lateral directions and at low density in the downgradient direction. Heterogeneous colonization led to empty zones. Particle tracking suggested rerouting of flow due to biomass growth. Microscale time-lapse measured filamentous growth rates (0.5 to 1.6 µm/min) were in good agreement with measured mesoscale colony expansion rates. Rather than the microscale concept of biomass developing at the surface of soil grains, filamentous growth may be better represented as mesoscale colonies spanning over several pores and separated from each other by open flow channels. Biological clogging might be prevented if such flow channels could be kept open in some manner.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Mesoscale and Microscale Observations of Biological Growth in a Silicon Pore Imaging Element

Dupin

McCarty

1999

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

Microbial Transport in the Subsurface

Ginn

Camesano

Scheibe

et al. 2005

Encyclopedia of Hydrological Sciences

View full text Add to dashboard Cite

We review the processes involved in the transport of bacteria in the saturated subsurface. Our goal is to communicate priority topics in the basic science of bacterial transport processes, and this leads to a focus on the nanometer to micron scales of observation. The scope of this review is the set of physical, chemical, and biological processes involved in the transport of individual cells, and the constitutive theory underlying conceptual depiction of these processes.

show abstract

An improved pore-scale biofilm model and comparison with a microfluidic flow cell experiment

et al. 2013

View full text Add to dashboard Cite

[1] This work presents a pore-scale biofilm model that solves the flow field using the lattice Boltzmann method, the concentration field of chemical species using the finite difference method, and biofilm development using the cellular automaton method. We adapt the model from a previous work and expand it by implementing biofilm shrinkage in the cellular automaton method. The new pore-scale biofilm model is then evaluated against a previously published pore-scale biofilm experiment, in which two microfluidic flow cells, one with a homogeneous pore network and the other with an aggregate pore network, were tested for aerobic degradation of a herbicide. The simulated biofilm distribution and morphology, biomass accumulation, and contaminant removal are generally consistent with the experimental data. Biofilm detachment in this model occurs when the local shear stress is above a critical value. We use the critical value from our previously published modeling study and find it works well in this case, even though we now have a different pore network and a different microbial species. We also use the model to show that the interaction between flow and biofilm growth is important to predict contaminant removal. The computational time of the new model is reduced 90% compared to our prior work due to implementation of biofilm shrinkage in the cellular automaton method. To the best of our knowledge, this is the first time that biofilm shrinkage has been incorporated into a pore-scale model for simulation of pollutant biodegradation in porous media.

show abstract

Biofilm morphology in porous media, a study with microscopic and image techniques

Cited by 27 publications

References 7 publications

Mesoscale and Microscale Observations of Biological Growth in a Silicon Pore Imaging Element

Mesoscale and Microscale Observations of Biological Growth in a Silicon Pore Imaging Element

Microbial Transport in the Subsurface

An improved pore-scale biofilm model and comparison with a microfluidic flow cell experiment

Contact Info

Product

Resources

About