Biofilm production by clinical Staphylococci strains from canine otitis

Moreira, Camila Alencar; Oliveira, Lis Christina de; Mendes, Marina Silveira; Santiago, Thiago de Melo; Barros, Eduardo B.; Carvalho, Cibele Barreto Mano de

doi:10.1590/s1517-83822012000100044

Cited by 12 publications

(13 citation statements)

References 7 publications

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“…In this study, all S. pseudintermedius clinical OE isolates produced biofilm, but 80% of these isolates (MSSP = 12, MRSP = 4) were weak biofilm producers regardless of their resistance profile. In contrast, a previous study found that only 39.3% of S. pseudintermedius (previously identified as S. intermedius ) isolated from canine OE were biofilm producers, but no further classification of biofilm formation was undertaken (Moreira et al, ). Another study revealed that 98% ( n = 140) of S. pseudintermedius isolated from dogs with skin, wound and surgical infections were biofilm producers with the majority (61%) being strong biofilm producers (Singh, Walker, Rousseau, & Weese, ).…”

Section: Discussionmentioning

confidence: 93%

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Chan

Hickey

Page³

et al. 2019

Vet Pharm & Therapeutics

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Otitis externa (OE) is a frequently reported disorder in dogs associated with secondary infections by Staphylococcus, Pseudomonas and yeast pathogens. The presence of biofilms may play an important role in the resistance of otic pathogens to antimicrobial agents. Biofilm production of twenty Staphylococcus pseudintermedius and twenty Pseudomonas aeruginosa canine otic isolates was determined quantitatively using a microtiter plate assay, and each isolate was classified as a strong, moderate, weak or nonbiofilm producer. Minimum biofilm eradication concentration (MBEC) of two ionophores (narasin and monensin) and three adjuvants (N‐acetylcysteine (NAC), Tris‐EDTA and disodium EDTA) were investigated spectrophotometrically (OD570nm) and quantitatively (CFU/ml) against selected Staphylococcus and Pseudomonas biofilm cultures. Concurrently, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of planktonic cultures were assessed. 16/20 of the S. pseudintermedius clinical isolates were weak biofilm producers. 19/20 P. aeruginosa clinical isolates produced biofilms and were distributed almost equally as weak, moderate and strong biofilm producers. While significant antibiofilm activity was observed, no MBEC was achieved with narasin or monensin. The MBEC for NAC ranged from 5,000–10,000 µg/ml and from 20,000–80,000 µg/ml against S. pseudintermedius and P. aeruginosa, respectively. Tris‐EDTA eradicated P. aeruginosa biofilms at concentrations ranging from 6,000/1,900 to 12,000/3,800 µg/ml. The MBEC was up to 16‐fold and eightfold higher than the MIC/MBC of NAC and Tris‐EDTA, respectively. Disodium EDTA reduced biofilm growth of both strains at concentrations of 470 µg/ml and higher. It can be concluded that biofilm production is common in pathogens associated with canine OE. NAC and Tris‐EDTA are effective antibiofilm agents in vitro that could be considered for the treatment of biofilm‐associated OE in dogs.

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Section: Discussionmentioning

confidence: 93%

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Chan

Hickey

Page³

et al. 2019

Vet Pharm & Therapeutics

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“…This suggested a higher pathogenicity for the strain, increasing resistance of bacteria and consequent recurrence of chronic otitis cases (OLIVEIRA et al, 2005). In another article, the author considers biofilm forming isolates presenting as black colonies in CRA, and reported the ability of S. intermedius isolates to form biofilms (11%) (MOREIRA et al, 2012). In the present study, we considered these colonies as only SL producers; although, in such colonies a high rate of biofilm formation has already been reported (MOREIRA et al, 2012).…”

Section: Discussionmentioning

confidence: 72%

Staphylococcal slime layers and biofilm from different origins

et al. 2019

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The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of capsule and biofilm, which contribute to its infectivity. The objective of this study was to observe the production of slime layer and formation of biofilm, which are related to the resistance to antimicrobial agents and presence of icaA and icaD genes, in 41 isolates of Staphylococcus spp. from different origins, provided by the Universidade Federal de Pelotas (UFPEL), Laboratório Regional de Diagnóstico (LRD). Strains of Staphylococcus spp. were cultivated in Congo red agar for capsule detection. Biofilm formation was detected using the 96-well microplate testing. Antimicrobial susceptibility testing was performed using the plate diffusion method. Part of the analyzed samples produced slime layer (36.6%) and formed biofilm (17.1%). However, six of those that formed biofilms were susceptible to the eight antibiotics tested in the antibiogram. In tests to determine the minimum bactericidal and inhibitory concentrations, gentamicin resistance of biofilm-forming strains was greater than that of non-forming strains. Ampicillin was the least effective antimicrobial drug (51%), followed by tetracycline (71%), neomycin (73%), and erythromycin (73%). Some isolates presented the icaA (6) and icaD (11) genes. Therefore, we suggested that the origin of an isolate can determine its expression of virulence factor and resistance to certain antibiotics.

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“…The ability of each strain to produce biofilm was quantified spectrophotometrically as previously described (8,9). Biofilm production was induced in a flat-bottomed well of a 96-well microtiter plate, using brain heart infusion broth (Oxoid, Basingstoke, Hampshire, England) enriched with 1% glucose.…”

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confidence: 99%

“…Biofilm production was induced in a flat-bottomed well of a 96-well microtiter plate, using brain heart infusion broth (Oxoid, Basingstoke, Hampshire, England) enriched with 1% glucose. The plates were incubated for 48 h at 37°C (8,9), and each well was dyed with 0.25% crystal violet, after washing with phosphate-buffered saline (PBS). Based on the obtained optical density values at 570 nm (OD 570 ), the strains were classified as nonproducers, weak producers, moderate producers, or strong producers, as described by Stepanovic et al (8).…”

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confidence: 99%

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In Vitro Activities of Amoxicillin-Clavulanate, Doxycycline, Ceftazidime, Imipenem, and Trimethoprim-Sulfamethoxazole against Biofilm of Brazilian Strains of Burkholderia pseudomallei

Bandeira

Moreira

Brilhante

et al. 2013

Antimicrob Agents Chemother

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eThis study aimed at investigating the in vitro activities of amoxicillin-clavulanate, doxycycline, ceftazidime, imipenem, and trimethoprim-sulfamethoxazole against Burkholderia pseudomallei in planktonic and biofilm forms, through broth microdilution and resazurin-based viability staining, respectively. In planktonic growth, the strains were susceptible to the drugs, while in biofilm growth, significantly higher antimicrobial concentrations were required, especially for ceftazidime and imipenem, surpassing the resistance breakpoints. These results highlight the importance of the routine evaluation of biofilm antimicrobial susceptibility.

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Biofilm production by clinical Staphylococci strains from canine otitis

Cited by 12 publications

References 7 publications

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Biofilm production by pathogens associated with canine otitis externa, and the antibiofilm activity of ionophores and antimicrobial adjuvants

Staphylococcal slime layers and biofilm from different origins

In Vitro Activities of Amoxicillin-Clavulanate, Doxycycline, Ceftazidime, Imipenem, and Trimethoprim-Sulfamethoxazole against Biofilm of Brazilian Strains of Burkholderia pseudomallei

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