Staphylococcal slime layers and biofilm from different origins

Cunha, Rodrigo Casquero; Rosa, Michelle Dias Hornes da; Silva, Cleomar da; Santos, Francisco Denis Souza

doi:10.1590/0103-8478cr20180783

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…Bacteria resistance is its ability to evade from the bactericidal or bacteriostatic mechanisms of a particular antibiotic, which reflects their structural or functional characteristics (34) . In this context, the identification of antibiotic bacteria resistance is relevant for an efficient antibiotic choice and successful treatment (35) .…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Tobramycin and Ciprofloxacin Against Bacterial Isolates in Canine Otitis Externa in Uberaba, Minas Gerais

et al. 2019

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Canine otitis externa is a common and recurrent disease in domestic dogs. Although not being life threatening, it can result in the overuse of antibiotics, increased bacterial resistance and reduction of options for treatment. This study aimed to determine: the principal characteristics (age, race, and gender) of dogs affected by otitis externa; the most frequently bacteria identified as being associated with it and their antimicrobial resistance profiles. A total of 221 bacterial isolates were identified by morphological staining and biochemical processes and tested against eleven antibiotics. Among the 148 dogs diagnosed with otitis externa, 51.4% (76/148) were males and 48.6% (72/148) females. The cases were detected mainly in dogs with age above 7 years (54.7%; 81/148. Among the races examined, poodles were the most numerous (14.84%; 22/148). The most common bacteria found were: coagulase-negative Staphylococcus (StCN), 33.9% (75/221); coagulase-positive Staphylococcus (StCP), 19% (42/221); Proteus spp., 17.2% (38/221) and Pseudomonas aeruginosa, 9.5% (21/221). Simultaneous resistance to more than three classes of antimicrobials were most frequently detected in P. aeruginosa (61.9%; 13/21) and Proteus spp (39.47%; 15/38). According to data obtained from this study, two veterinary medications found to be most effective for topical treatment were tobramycin and ciprofloxacin.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of Tobramycin and Ciprofloxacin Against Bacterial Isolates in Canine Otitis Externa in Uberaba, Minas Gerais

et al. 2019

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“…Bacterial biofilm present protective functions against dehydration, bacteriophage infection and antimicrobial agents. Importantly, the biofilms are a major concern in the food industry for their potential to resist antimicrobial treatments, facilitating pathogenic contamination and food degradation [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation Ability and Presence of Adhesion Genes among Coagulase-Negative and Coagulase-Positive Staphylococci Isolates from Raw Cow’s Milk

Gajewska

Chajęcka‐Wierzchowska

2020

Pathogens

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The capacity for biofilm formation is one of the crucial factors of staphylococcal virulence. The occurrence of biofilm-forming staphylococci in raw milk may result in disturbances in technological processes in dairy factories as well as the contamination of finished food products. Therefore, this study aimed to determine the prevalence and characteristics of staphylococcal biofilm formation in raw milk samples and to explore the genetic background associated with biofilm formation in those isolates. The material subjected to testing included 30 cow’s milk samples acquired from farms in the central part of Poland. A total of 54 staphylococcal strains were isolated from the samples, of which 42 were classified as coagulase-negative (CoNS) staphylococci belonging to the following species: S. haemolyticus, S. simulans, S. warneri, S. chromogenes, S. hominis, S. sciuri, S. capitis, S. xylosus and S. saprophyticus, while 12 were classified as S. aureus. The study examined the isolates’ capacity for biofilm formation and the staphylococcal capacity for slime production and determined the presence of genetic determinants responsible for biofilm formation, i.e., the icaA, icaD, bap and eno and, additionally, among coagulase-negative staphylococci, i.e., the aap, bhp, fbe, embP and atlE. Each tested isolate exhibited the capacity for biofilm formation, of which most of them (79.6%) were capable of forming a strong biofilm, while 5.6% formed a moderate biofilm, and 14.8% a weak biofilm. A capacity for slime production was demonstrated in 51.9% isolates. Most of the tested staphylococcal strains (90.7%) had at least one of the tested genes. Nearly half (47.6%) of the CoNS had the eno gene, while for S. aureus, the eno gene was demonstrated in 58.3% isolates. The frequency of the bap gene occurrence was 23.8% and 25% in CoNS strains and S. aureus, respectively. The fbe gene was demonstrated in only three CoNS isolates. The presence of the icaA was only demonstrated in CoNS strains (24.1%), while the icaD was found in both CoNS strains (21.4%) and S. aureus (100%). Among the CoNS, the presence of the embP (16.7%), aap (28.6%) and atlE (23.8%) was demonstrated as well. The obtained study results indicate that bacteria of the Staphylococcus spp. genus have a strong potential to form a biofilm, which may pose a hazard to consumer health.

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“…Esta relação entre resistência a antimicrobianos e a formação de biofilme já foi relatada em estudos anteriores, onde isolados de Staphylococcus aureus resistentes a antimicrobianos da classe dos beta-lactâmicos também obtiveram resultado positivo para formação de biofilme (Marques, 2016;Cunha., et al, 2019). Os resultados de Cad.…”

Section: Resultsunclassified

“…A eficiência do método Ágar vermelho congo (CRA) para identificação de cepas de Staphylococcus spp. facilidade de execução e baixo custo tornam o método indicado para rastreamento de cepas com potencial para produção de biofilmes (Cunha, et al, 2019).…”

Section: Introductionunclassified

Formação de biofilmes por Staphylococcus aureus multirresistentes isolados de leite de vacas com mastite

et al. 2021

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Staphylococcus aureus provenientes de leite de vacas com mastite subclínica e fenotipicamente resistentes a antimicrobianos da classe dos beta-lactâmicos foram testadas quanto a capacidade em produzir biofilme. Foi procedido o cultivo e análise em Ágar vermelho congo (CRA) e Teste de Aderência em Placas (AEP). Também foi avaliada a associação entre os testes CRA e AEP e a associação de cada um destes testes com a frequência das cepas potencialmente produtoras de biofilme resistentes aos beta-lactâmicos. Como resultado, 65,2% (15) dos 23 isolados testaram positivo para formação de biofilme em CRA e 60,86% (14) em AEP. Não houve associação entre os resultados do teste CRA e o teste AEP, também não houve associação com a resistência aos antimicrobianos beta-lactâmicos (RBL). Já entre o teste de AEP e RBL houve associação. Considera-se que a capacidade em formar biofilme um poderoso mecanismo de resistência e ressalta-se a importância do controle microbiológico na produção.

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Staphylococcal slime layers and biofilm from different origins

Cited by 7 publications

References 19 publications

Effectiveness of Tobramycin and Ciprofloxacin Against Bacterial Isolates in Canine Otitis Externa in Uberaba, Minas Gerais

Effectiveness of Tobramycin and Ciprofloxacin Against Bacterial Isolates in Canine Otitis Externa in Uberaba, Minas Gerais

Biofilm Formation Ability and Presence of Adhesion Genes among Coagulase-Negative and Coagulase-Positive Staphylococci Isolates from Raw Cow’s Milk

Formação de biofilmes por Staphylococcus aureus multirresistentes isolados de leite de vacas com mastite

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