Biofilm production by Haemophilus influenzae and Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media

Vermee, Q.; Cohen, Robert; Hays, Constantin; Varon, Emmanuelle; Bonacorsi, Stéphane; Béchet, Stéphane; Thollot, Franck; Corrard, F.; Poyart, Claire; Lévy, Corinne; Raymond, Josette

doi:10.1186/s12879-018-3657-9

Cited by 32 publications

(23 citation statements)

References 49 publications

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“…One study found that almost 70 % of S. pneumoniae isolated from the nasopharynx of children with AOM produced biofilms and 33.5 % of them were strong biofilm producers [34]. Bacteria in biofilms are more resistant to the action of antimicrobials than planktonic forms [35].…”

Section: Discussionmentioning

confidence: 99%

“…Pneumococcus, which colonizes the nasopharynx asymptomatically, can sense nasopharyngeal environmental changes caused by viral infections by upregulating specific genes involved in the formation of biofilms, dissemination to other sites, and evasion of the host immune system [ 33 ]. One study found that almost 70 % of S. pneumoniae isolated from the nasopharynx of children with AOM produced biofilms and 33.5 % of them were strong biofilm producers [ 34 ]. Bacteria in biofilms are more resistant to the action of antimicrobials than planktonic forms [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

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Nasopharyngeal carriage rates and serotype distribution of Streptococcus pneumoniae among school children with acute otitis media in Central Java, Indonesia

Wahyono

Khoeri

Darmawan

et al. 2021

Access Microbiology

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Streptococcus pneumoniae is a common bacterial pathogen that causes acute otitis media (AOM) in children. In this study, we investigated nasopharyngeal carriage rates and serotype distributions of S. pneumoniae among school children with AOM in Banyumas Regency, Central Java, Indonesia, from 2018 to 2019. Nasopharyngeal swab specimens and demographic data were collected from 122 children between the ages of 6 and 12. The specimens were cultured for the identification of S. pneumoniae , and serotyping was performed using a sequential multiplex PCR assay. We found that the S. pneumoniae carriage rate was 73 % (89/122) among children with AOM. Serotypes 23A (11 %) and 6A/6B (10 %) were the most common serotypes among the 91 cultured S. pneumoniae strains, followed by 3 (8 %), 14 (7 %), 6C/6D (7 %), 11A/11D (6 %), 15B/15C (4 %) and 35 B (4 %). Moreover, 41 % of the strains could be covered by the 13-valent pneumococcal conjugate vaccine, PCV13. In conclusion, high nasopharyngeal carriage rates of S. pneumoniae were found in school children with AOM, with almost half of the strains being the vaccine-type. This finding provides a baseline for nasopharyngeal carriage of S. pneumoniae in school children with AOM and supports the implementation of pneumococcal conjugate vaccines in Indonesia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nasopharyngeal carriage rates and serotype distribution of Streptococcus pneumoniae among school children with acute otitis media in Central Java, Indonesia

Wahyono

Khoeri

Darmawan

et al. 2021

Access Microbiology

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“…Chronic bacterial infections are a significant problem for patients with mucosal clearance defects, including patients with cystic fibrosis, COPD and preceding viral infection (16,48,49). Bacterial biofilms, which display heightened resistance to antibiotics, oxidative stress, and house persister cells, contribute significantly to the development of chronic infections (24,30,(49)(50)(51).…”

Section: Discussionmentioning

confidence: 99%

Nontypeable Haemophilus influenzae redox recycling of protein thiols promotes resistance to oxidative killing and bacterial survival in biofilms in a smoke related infection model

Hunt

Gaggar

et al. 2021

Preprint

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Smoke exposure is a risk factor for community acquired pneumonia, which is typically caused by host adapted opportunists like nontypeable Haemophilus influenzae (NTHi). Genomic analyses of NTHi revealed homologs of enzymes involved in thiol metabolism, which can have key roles in oxidant resistance. Using a clinical NTHi isolate (NTHi 7P49H1), we generated isogenic mutant bacterial strains in which homologs of glutathione reductase (NTHI 0251), thiol peroxidase (NTHI 0361), thiol peroxidase (NTHI 0907), thioredoxin reductase (NTHI 1327) and glutaredoxin/peroxiredoxin (NTHI 0705) were inactivated. Bacterial protein analyses revealed significant increases in protein oxidation after oxidative stress for all the mutant strains. Similarly, each of these mutants were less resistant to oxidative killing compared with the parental strain; these phenotypes were reversed by genetic complementation. Quantitative confocal analysis of biofilms showed reducted biofilm thickness and density, and significant sensitization of bacteria within the biofilm structure to oxidative killing for thiol mutant strains. Smoke-exposed mice infected with NTHi 7P49H1 showed significantly increased lung bacterial load, as compared to control mice. Immunofluorescent staining of lung tissues showed NTHi communities on the lung mucosa, interspersed with host neutrophil extracellular traps; these bacteria had surface moieties associated with the Hi biofilm matrix, and transcript profiles consistent with NTHi biofilms. In contrast, infection with the panel of NTHi mutants showed significant decrease in lung bacterial load. Comparable results were observed in bactericidal assays with neutrophil extracellular traps in vitro. Thus, we conclude that thiol mediated redox homeostasis promotes persistence of NTHi within biofilm communities.ImportanceChronic bacterial respiratory infections are a significant problem for smoke exposed individuals, especially those with chronic obstructive pulmonary disease (COPD). These infections often persist despite antibiotic use. Thus, the bacteria remain and contribute to the development of inflammation and other respiratory problems. Respiratory bacteria often form biofilms within the lungs, while growing in a biofilm their antibiotic and oxidative stress resistance is incredibly heightened. It is well documented that redox homeostasis genes are upregulated during this phase of growth. Many common respiratory pathogens such as NTHi and Streptococcus pneumoniae are reliant on scavenging from the host the necessary components they need to maintain these redox systems. This work here begins to lay down the foundation for exploiting this requirement and thiol redox homeostasis pathways of these bacteria as a therapeutic target for managing chronic respiratory bacterial infections, which are resistant to traditional antibiotic treatments alone.

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“…H. influenzae and S. pneumoniae showed alterations in gene expression when in co-culture, but expression levels were also dependent of the growth environment with genes encoding lactose and glycerol utilization, and sugar transport proteins differing in the levels expressed (Tikhomirova et al 2015). The biofilm forming ability of H. influenzae and S. pneumoniae strains isolated from the nasopharynx of children with AOM revealed that almost 65% of H. influenzae and 67% of S. pneumoniae isolates produced biofilms (Vermee et al 2019). Interestingly, better H. influenzae biofilm-producing strains were, in general, isolated from samples containing also S. pneumoniae but this correlation was not valid for S. pneumoniae strains.…”

Section: Multispeciesmentioning

confidence: 99%

Otitis media pathogens – A life entrapped in biofilm communities

Silva

Sillankorva

2019

Critical Reviews in Microbiology

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Otitis media is a group of inflammatory diseases of the middle ear with great impact on children worldwide. The most common reported bacterial pathogens are Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis. Over the last years, the role of biofilms formed by otopathogens that contribute to otitis media recurrence and chronicity has been established. An improved understanding of the properties of biofilms formed by these bacteria, which factors influence them, and how these affect the host inflammatory response is important for the development of novel strategies for the treatment of otitis media. This review focuses on the biofilm nature that the most prevalent otopathogens adopt in otitis media infections. In addition, new treatment approaches targeting biofilms are highlighted.

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Biofilm production by Haemophilus influenzae and Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media

Cited by 32 publications

References 49 publications

Nasopharyngeal carriage rates and serotype distribution of Streptococcus pneumoniae among school children with acute otitis media in Central Java, Indonesia

Nasopharyngeal carriage rates and serotype distribution of Streptococcus pneumoniae among school children with acute otitis media in Central Java, Indonesia

Nontypeable Haemophilus influenzae redox recycling of protein thiols promotes resistance to oxidative killing and bacterial survival in biofilms in a smoke related infection model

Otitis media pathogens – A life entrapped in biofilm communities

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