Biofilm production in relation to extended spectrum beta-lactamase production and antibiotic resistance among uropathogenic Escherichia coli

Khatri, Samip; Pant, Narayan Dutt; Neupane, Sanjeev; Bhandari, Suman; Banjara, Megha Raj

doi:10.3126/jmcjms.v5i1.17989

Cited by 4 publications

(5 citation statements)

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“…Among 60 E. coli isolates, 31.66% were strong biofi lm producers, 21.66% moderately positive, 21.66% were weak ones and 25% were biofi lm non-producers by using microtitre plate method which were in accordance with the fi ndings of Neupane et al (2016) and Khatri et al (2017).…”

Section: Discussionsupporting

confidence: 77%

“…The overall prevalence of E. coli in urine samplesfrom UTI suspected patients was15% (60/400). Neupane et al (2016) and Khatri et al (2017) showed very similar report of 15.5% and 14.1% respectively. In this study the prevalence of UTI by E. coli was higher in female population than in male population which was statistically signifi cant (p<0.05) which is consistent with many other studies.…”

Section: Discussionsupporting

confidence: 63%

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Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal

et al. 2019

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Objectives: The main objective of this study was to determine the prevalence of Escherichia coli among urinary tract infection (UTI) suspected patients visiting tertiary care hospital and to assess the biofilm producing ability of E. coli isolates. Methods: A prospective cross-sectional study was carried out in Biratnagar Metropolitan city, Eastern Nepal from December 2018 to May 2019. During the study 400 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and identified. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to CLSI (2011) guidelines. Biofilm assays were performed by microtitre plate method. Results: This study reported 15% prevalence of E. coli out of 400 urine samples. 100% of E. coli isolates showed resistance to both Ampicillin and Amoxicillin while 100% were sensitive to Chloramphenicol. 70% (42/60) isolates were Multi Drug Resistance (MDR)E. coli. The maximum isolates (86.66%) were found to be biofilm producers by microtitre plate method. Resistance to other antibiotics such as Nalidixic acid (71.11% vs 46.66%), Norfloxacin (53.33% vs 46.66%), Cotrimoxazole (42.22% vs 26.66%) was comparatively higher among biofilm producers than non-biofilm producers. There was a significance of association between biofilm and MDR (p<0.05). Conclusion: There is relation between the ability of biofilm formation and drug resistance in the bacterium resulting to the failure of antibacterial drugs.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 63%

Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal

et al. 2019

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“…The previous studies of Neupane et al, (2016); Khatri et al, (2017) confirmed the low prevalence of E. coli isolates recovered from urine samples of UTI suspected patients. Basically, the usual diagnosis of UTI is based on a quantitative estimation of bacteria in urine culture that yield greater than 10 5 cfu/ ml (Kass, 1957).…”

Section: Epidemiologysupporting

confidence: 67%

Uropathogenic Escherichia coli in urinary tract infections: A review on epidemiology, pathogenesis, clinical manifestation, diagnosis, treatments and prevention

Shrestha

Tumbahangphe

Shakya

et al. 2022

Novel Research in Microbiology Journal

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Urinary tract infections (UTIs) are common pathological conditions observed in hospital settings and communities. Uropathogenic Escherichia coli (UPEC) is the causative agent of most of the UTIs, such as pyelonephritis and cystitis. The infectious complications may cause acute renal failure affecting both the healthy and renal transplant patient's. The untreated patients with UTI may exhibit septicemia and bacteremia. Furthermore, the multidrug resistance patterns of UPEC may result in severe septic shock. Factors that contribute to the pathogenesis of UPEC include; secreted proteins, haemolysins, capsule, lipopolysaccharides, biofilm, fimbriae adhesions and iron acquisition systems. In spite of several host protection mechanisms; however, UPEC may persist inside the urinary tract and serve as a reservoir of recurrent infections and complications. Early diagnosis and prompt treatment of UTI with broad spectrum antibiotics are essential before this infection causes other medical complications. Generally, in clinical settings, diagnosis of UTIs involves bacterial culture and antibiotic susceptibility assay, in addition to other medical examinations, which aid the physicians to prescribe the appropriate drugs and measures during UTIs treatments. This review aims to understand the epidemiology, pathogenesis, clinical manifestation, diagnosis, treatment and preventive measures of UTIs caused by the uropathogenic E. coli.

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“…(Abernethy et al 2017). Resistance to ciprofloxacin was significantly higher in developing countries (Ethiopia -85.5%, Nepal -64.6%, Pakistan -60.8%, Mongolia -58.1%, Jordan -55.5%) (Ali et al 2016, Khatri et al 2017Munkhdelger et al 2017;Regasa Dadi et al 2018;Shakhatreh et al 2018) than in developed countries (USA -5.1%, Germany -10.5%, Switzerland -17.4%, France -24.8%) (Lavigne et al 2016;Erb et al 2018;Hitzenbichler et al 2018;Yamaji et al 2018). The study conducted in Switzerland showed also that patient age over 65 years was associated with higher E. coli resistance to ciprofloxacin (Erb et al 2018).…”

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confidence: 98%

Antibiotic Resistance Among Uropathogenic Escherichia coli

Kot

2019

Polish Journal of Microbiology

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131

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Urinary tract infections (UTIs) belong to the most common community-acquired and nosocomial infections. A main etiological factor of UTIs is uropathogenic Escherichia coli (UPEC). This review describes the current state of knowledge on the resistance of UPEC to antibiotics recommended for the treatment of UTIs based on the available literature data. Nitrofurantoin and fosfomycin are recommended as first-line therapy in the treatment of uncomplicated cystitis, and the resistance to these antimicrobial agents remains low between UPEC. Recently, in many countries, the increasing resistance is observed to trimethoprim-sulfamethoxazole, which is widely used as the first-line antimicrobial in the treatment of uncomplicated UTIs. In European countries, the resistance of UPEC to this antimicrobial agent ranges from 14.6% to 60%. The widespread use of fluoroquinolones (FQs), especially ciprofloxacin, in the outpatients is the cause of a continuous increase in resistance to these drugs. The resistance of UPEC to FQs is significantly higher in developing countries (55.5–85.5%) than in developed countries (5.1–32.0%). Amoxicillin-clavulanic acid is recommended as first line-therapy for pyelonephritis or complicated UTI. Resistance rates of UPEC to amoxicillin-clavulanic acid are regionally variable. In European countries the level of resistance to this antimicrobial ranges from 5.3% (Germany) to 37.6% (France). Increasing rates of UPEC resistance to antimicrobials indicate that careful monitoring of their use for UTI treatment is necessary.

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Biofilm production in relation to extended spectrum beta-lactamase production and antibiotic resistance among uropathogenic Escherichia coli

Abstract: Not available. Janaki Medical College Journal of Medical Sciences (2017) Vol. 5(1): 61-63

Cited by 4 publications

References 4 publications

Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal

Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal

Uropathogenic Escherichia coli in urinary tract infections: A review on epidemiology, pathogenesis, clinical manifestation, diagnosis, treatments and prevention

Antibiotic Resistance Among Uropathogenic Escherichia coli

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