Biofilms of <i>Candida</i> spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?

Brilhante, Raimunda Sâmia Nogueira; Bittencourt, Paula Vago; Castelo-Branco, Débora de Souza Collares Maia; Guedes, Gláucia Morgana de Melo; Oliveira, Jonathas Sales de; Alencar, Lucas Pereira de; Cordeiro, Rossana de Aguiar; Pinheiro, Mariana; Nogueira-Filho, Evilázio Fernandes; Pereira-Neto, Waldemiro de Aquino; Sidrim, José Júlio Costa; Rocha, Marcos Fábio Gadelha

doi:10.1111/vop.12465

Cited by 14 publications

(14 citation statements)

References 38 publications

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“…In addition, our results showed that 11.4% and 8.6% of C. albicans isolates were resistant to FLC and ITC, respectively. These findings are consistent with those of previous studies conducted in Portugal and China 23,24 .…”

Section: Discussionsupporting

confidence: 94%

“…In this study, C. albicans isolates with the highest biofilm-forming ability among the ones tested were selected and analyzed through scanning electron microscopy (SEM), according to a previously described method 23 . After incubation and removal of free cells by washing, biofilms and sticky cells were fixed with glutaraldehyde and incubated at 4°C overnight.…”

Section: Scanning Electron Microscopic (Sem) Study Of Candida Biofilmsmentioning

confidence: 99%

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Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: The aim of this study was to evaluate some virulence factors in Candida albicans isolates from patients with onychomycosis and determine the correlation between these factors and the antifungal resistance profile. Methods: Seventy species of C. albicans were confirmed using polymerase chain reaction amplification of the HWP1 gene. According to the Clinical & Laboratory Standards Institute guidelines, the susceptibility profile of four antifungal agents was investigated, and the production of aspartyl protease, phospholipase, haemolysin, and biofilm was determined. The correlation between these profiles was also investigated. Results: The isolates indicated different levels of resistance and production of virulence factors. Significant correlations were observed between the minimum inhibitory concentration (MIC) of fluconazole/itraconazole and biofilm production, between phospholipase production and fluconazole/ itraconazole MIC, and between fluconazole MIC and hemolytic activity in C. albicans isolates. The results also showed significant correlations between phospholipase activity and biofilm production. Conclusions: Our findings will contribute to a better understanding of the pathogenesis of C. albicans and characterize the relationship between virulence factors and antifungal resistance, which may suggest new therapeutic strategies considering the possible involvement of the virulence mechanism in the effectiveness of treatment.

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Section: Discussionsupporting

confidence: 94%

Section: Scanning Electron Microscopic (Sem) Study Of Candida Biofilmsmentioning

confidence: 99%

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…The OD values were recorded for all tested isolates and negative controls. The isolates were then classified as non-biofilm producers when OD 590 nm ≤ ODc, weak biofilm producers, when ODc < OD 590 nm ≤ 2×ODc, moderate biofilm producers, when 2×ODc< OD 590 nm ≤ 4×ODc, and strong biofilm producers when OD 590 nm >4× ODc (Brilhante et al, 2017).…”

Section: Screening For Biofilm Formationmentioning

confidence: 99%

Impact of Quorum Sensing Molecules at Supra-physiological Concentrations on Virulence Factors of Multiple Drug Resistant Candida Clinical Isolates

El-Banna¹,

Sonbol²,

Al-Kharsawy³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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Candida albicans a polymorphic fungus which have the ability to develop as yeast, pseudohyphae and/or true hyphae forms. Candida isolates (n=34) were collected from different clinical specimens and identification to the species level was performed. Isolates were subjected to antifungal susceptibility testing against (nystatin, amphotericinB, Itraconazole, fluconazole, terbinafine, and caspofungin) and determination of their minimum inhibitory concentrations (MICs) were carried out according to the European Committee of Antimicrobial Susceptibility Testing (EUCAST, 2015) strategies. Virulence factors including secreted hydrolases (phospholipase and aspartyl protease) and biofilm production were evaluated. The hyphal form of Candida is themain role in infection process during its invasion to mucosal membrane. A group of genes participate in monitoring of hyphae formation in C. albicans, includingsap5 and hwp1.Farnesol compound is a quorum sensing molecule which prevents converting of yeast-to-hyphae form. The aim of our study was to determine farnesol effect in yeast-to-hyphae shift, and its related gene expressions in C. albicans using qRT-PCR. Real time-PCR analysis revealed that 1/2 MIC of farnesol has significantly decreased the expressionofsap5and hwp1genes in comparison to control group (p<0.05).

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“…9). Hefen hingegen können häufig an ihrer Sprossung erkannt werden, hierbei zweigen von einer rundlichen Mutterzelle kleinere, ebenfalls rundliche Sprosszellen ab [14]. Bereits wenige Pilzhyphen in der Hornhautzytologie sind beweisend für eine Keratomykose [13], allerdings schließt der fehlende Nachweis das Vorliegen einer Keratomykose nicht aus.…”

Section: Probenentnahmeunclassified

Diagnostik und Management der equinen Keratomykose

Weiß¹

2020

pferde spiegel

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Biofilms of Candida spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?

Abstract: The results demonstrate that Candida from the ocular conjunctiva of horses can pose as a risk to animal health as they are capable of forming biofilms, which are commonly involved in fungal keratitis.

Cited by 14 publications

References 38 publications

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Impact of Quorum Sensing Molecules at Supra-physiological Concentrations on Virulence Factors of Multiple Drug Resistant Candida Clinical Isolates

Diagnostik und Management der equinen Keratomykose

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