Biofilms produced by Burkholderia cenocepacia: influence of media and solid supports on composition of matrix exopolysaccharides

Pellizzoni, Elena; Ravalico, Fabio; Scaini, Denis; Delneri, Ambra; Rizzo, Roberto; Cescutti, Paola

doi:10.1099/mic.0.000214

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…It is of low virulence and is a frequent colonizer of fluids. In a study [13] , it was reported that even the non-mucoidal stage of the organism is capable of producing biofilms effectively. Bacillus megaterium is considered a grampositive, endospore-forming, rod-shaped, aerobic, and saprophytic bacteria that produces a variety of enzymes and proteins.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Study on the Efficacy of Copper Antifouling Coat on Biofilm-Formingorganisms in Industrial Cooling Towers

2020

MLU

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Cooling water systems serve as an ideal aquatic environment for microorganisms to proliferate and colonize. Biofouling and scaling are two major factors that adversely influence the water-cooling efficacy of industrial cooling towers, as they not only reduce the heat transfer capacity but also enhance corrosion. The present study focuses on the efficacy of copper antifouling coat applied over biofilm-forming organisms in industrial cooling towers, with a special emphasis on the quorum-sensing auto inducers and the EPS structure of organisms capable of forming the biofilm. Study results demonstrated sufficiently that copper coating has strong antifouling properties and the potential to prevent the growth of biofilm-forming organisms in industrial cooling tower systems. N-Acyl Homoserine Lactones is a major component produced bygramnegative biofilm-forming organisms, and the EPS is an important componentof all the organisms that form biofilm. The study findings led to the conclusion that use of copper antifouling paint can reduce biofilm formation and its signaling molecules in industrial cooling towers.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Study on the Efficacy of Copper Antifouling Coat on Biofilm-Formingorganisms in Industrial Cooling Towers

2020

MLU

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“…Afterward, the probe was allowed to approach and come into contact with the biofilm and the substrate using AFM (AFM, Park Systems, XE-100). The thickness of biofilm was obtained from the relative height between the biofilm skeleton and the substrate . For each biofilm, the measurements were taken at least three different locations and repeated for three times at the same location.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

et al. 2018

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Biocides can effectively kill bacteria; however, whether the dead bacterial cells left on the surface influence the later growth of biofilm is unknown. In this study, we have cultured Pseudomonas aeruginosa (PAO1) biofilm on their dead siblings and have investigated their evolution by using magnetic force modulation atomic force microscopy (MF-AFM). The time dependence of the biofilm thickness indicates that the deposited dead siblings can slow down the growth of PAO1 biofilm. The biofilm growing on dead bacteria layers is softer in comparison with those upon alive siblings, as reflected by the static elastic modulus ( E) and dynamic stiffness ( k) scaled to the disturbing frequency ( f) as k = k f, where k is the scaling factor and γ is the power-law exponent. We reveal that the smaller population instead of the variation of extracellular polymeric substances (EPS) within the biofilm upon the dead siblings is responsible for the softer biofilm. The present study provides a better understanding of the biofilm formation, thus, making it significant for designing antimicrobial medical materials and antifouling coatings.

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“…Burkholderia can produce extracellular polysaccharides or exopolysaccharides (EPSs), the high-molecular-weight sugarbased polymers that are synthesized and secreted by multi microorganisms.EPSs have the function as a scaffold of biofilms to cross-link the bacterial cells together and make bacterial adaptation to different stress conditions [19,20]. Pellizzoni et alreported that B. cenocepacia in a non-mucoid state could form biofilm containing EPSs [21].…”

Section: Exopolysaccharides (Epss)mentioning

confidence: 99%

Biofilm Targeting Strategy in the Eradication of Burkholderia Infections: A Mini-Review

Gunardi¹,

Timotius²,

Natasha³

et al. 2021

TOMICROJ

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Burkholderia are intracellular pathogenic bacteria which can produce biofilm. This biofilm protects the intracellular pathogenic bacteria from antibiotic treatment and the immunological system of the host. Therefore, this review aims to describe the capacity of Burkholderia to form a biofilm, the regulation of its biofilm formation, the efficacy of antibiotics to eradicate biofilm, and the novel therapy which targets its biofilm. Burkholderia's biofilm is characterized by its lipopolysaccharides, exopolysaccharides (EPSs), biofilm-associated proteins, and eDNA. Its regulation is made by quorum sensing, c-di-AMP, sRNA, and two component systems. Many antibiotics have been used as sole or mixture agents; however, they are not always effective in eradicating the biofilm-forming Burkholderia. Inhibitors of quorum sensing and other non-conventional antibiotic approaches are promising to discover effective treatment of Burkholderia infections.

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Biofilms produced by Burkholderia cenocepacia: influence of media and solid supports on composition of matrix exopolysaccharides

Cited by 9 publications

References 49 publications

A Comprehensive Study on the Efficacy of Copper Antifouling Coat on Biofilm-Formingorganisms in Industrial Cooling Towers

A Comprehensive Study on the Efficacy of Copper Antifouling Coat on Biofilm-Formingorganisms in Industrial Cooling Towers

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

Biofilm Targeting Strategy in the Eradication of Burkholderia Infections: A Mini-Review

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