Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment

Abstract

A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.

Red Lentil (Lens culinaris) Extract as a Novel Natural Coagulant for Turbidity Reduction: An Evaluation, Characterization and Performance Optimization Study

“…All three lentil extracts were highly carbonaceous materials which mainly made up of carbon and oxygen. The elemental composition results were found to be in close agreement with the natural coagulant reported by R. Ahmad and Haseeb [14] and Agunbiade et al [15] where carbon and oxygen were the major components for natural coagulant. Some inorganic elements, i.e., calcium (Ca) was detected in trace amount for red lentil extract and potassium (K) for red and brown lentil extracts.…”

Removal of Nutrients from Fertilizer Plant Wastewater Using Scenedesmus sp.: Formation of Bioflocculation and Enhancement of Removal Efficiency

“…Similarly, higher removal percentages of nitrate, phosphates, and COD were reported to associate with bioflocculation process of microalgae [13,18,19,38]. In general, algal cell density in flocculation forms is always higher than free forms [20,21]. e high removal efficiency in wastewater treatment with AFL could be attributed to the availability of sufficient oxygen for further mineralization of organic carbon and nutrients because of the high concentration of microalga cells in flocculation formation.…”

“…erefore, MBF and FAMP have been used to improve nutrient removal and effluent recovery in aerobic-activated sludge technologies [13,15,17]. e bioflocculation of microalgae and bacteria have been intensively studied and reviewed for recent advances and perspectives [18][19][20]. Besides, MBF can be used as a promising low-cost method for harvesting microalga biomass [14,21].…”

Optimized Use of Ferric Chloride and Sesbania Seed Gum (SSG) as Sustainable Coagulant Aid for Turbidity Reduction in Drinking Water Treatment

“…This explains the presence of carbon and oxygen structures, as per the FTIR results. Often, carbon and oxygen are the major compositions in bioflocculants such as guar gum [32] and Streptomyces platensis [33]. Bello, et al [34] and Xiong, et al [35] reported that polymers with a high composition of carbon generally exhibit good adsorption ability.…”