Biofouling control using microparticles carrying a biocide

Ferreira, Carla; Rosmaninho, R.; Simões, Manuel; Pereira, Maria do Carmo; Bastos, Margarida; Nunes, Olga C.; Coelho, Manuel A. N.; Melo, L. F.

doi:10.1080/08927010903419630

Cited by 31 publications

(22 citation statements)

References 39 publications

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“…The biofilm habitat represents a primary mode of growth for most microorganisms on earth (Flemming 2002;Ferreira et al 2009). It has been shown that biofilms are more resistant to toxic chemicals such as heavy metals and antimicrobial agents than planktonic cells (Choi et al 2010).…”

Section: Effect Of Biogenic Silver (Bio-ag 0-6 ) On Established Biofilmsmentioning

confidence: 99%

The antibacterial and anti-biofouling performance of biogenic silver nanoparticles byLactobacillus fermentum

et al. 2014

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Biofouling is a major challenge in the water industry and public health. Silver nanoparticles (AgNPs) have excellent antimicrobial properties and are considered to be a promising anti-biofouling agent. A modified method was used to produce small sized and well-dispersed biogenic silver nanoparticles with a mean size of ~6 nm (Bio-Ag0-6) using Lactobacillus fermentum. The morphology, size distribution, zeta potential and oxidation state of the silver were systematically characterized. Determination of minimal inhibitory and bactericidal concentration results revealed that biogenic silver Bio-Ag(0-6) can effectively suppress the growth of the test bacteria. Additionally, the inhibition effects of Bio-Ag(0-6) on biofilm formation and on established biofilms were evaluated using P. aeruginosa (ATCC 27853) as the model bacterium. The results from microtiter plates and confocal laser scanning microscopy demonstrated that Bio-Ag(0-6) not only exhibited excellent antibacterial performance but also could control biofilm formation and induce detachment of the bulk of P. aeruginosa biofilms leaving a small residual matrix.

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Section: Effect Of Biogenic Silver (Bio-ag 0-6 ) On Established Biofilmsmentioning

confidence: 99%

The antibacterial and anti-biofouling performance of biogenic silver nanoparticles byLactobacillus fermentum

et al. 2014

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“…Biofilms were grown on 24 ASI 316 stainless steel (SS) slides (2.5 cm 6 2.5 cm and 1 mm thick) that were hung within the chemostat system ( Figure 1). The chemostat system was operated at 27 + 28C, with aeration (air flow rate -0.350 min 71 ) and stirring (VWR, VMS-C7 Advanced; 120 rpm; stirrer length -2.5 cm), according to previous studies (Pereira and Vieira, 2001;Simo˜es et al 2003a;Ferreira et al 2010). The biofilms were allowed to grow for 6 days, which was the time needed to reach the steady state in terms of cell density, metabolic activity and mass (Pereira and Vieira 2001).…”

Section: Biofilm Reactormentioning

confidence: 99%

The effects of glutaraldehyde on the control of single and dual biofilms ofBacillus cereusandPseudomonas fluorescens

et al. 2011

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Glutaraldehyde (GLUT) was evaluated for control of single and dual species biofilms of Bacillus cereus and Pseudomonas fluorescens on stainless steel surfaces using a chemostat system. The biofilms were characterized in terms of mass, cell density, total and matrix proteins and polysaccharides. The control action of GLUT was assessed in terms of inactivation and removal of biofilm. Post-biocide action was characterized 3, 7, 12, 24, 48 and 72 h after treatment. Tests with planktonic cells were also performed for comparison. The results demonstrated that in dual species biofilms the metabolic activity, cell density and the content of matrix proteins were higher than those of either single species. Planktonic B. cereus was more susceptible to GLUT than P. fluorescens. The biocide susceptibility of dual species planktonic cultures was an average of each single species. Planktonic cells were more susceptible to GLUT than their biofilm counterparts. Biofilm inactivation was similar for both of the single biofilms while dual biofilms were more resistant than single species biofilms. GLUT at 200 mg l(-1) caused low biofilm removal (<10%). Analysis of the post-biocide treatment data revealed the ability of biofilms to recover their activity over time. However, 12 h after biocide application, sloughing events were detected for both single and dual species biofilms, but were more marked for those formed by P. fluorescens (removal >40% of the total biofilm). The overall results suggest that GLUT exerts significant antimicrobial activity against planktonic bacteria and a partial and reversible activity against B. cereus and P. fluorescens single and dual species biofilms. The biocide had low antifouling effects when analysed immediately after treatment. However, GLUT had significant long-term effects on biofilm removal, inducing significant sloughing events (recovery in terms of mass 72 h after treatment for single biofilms and 42 h later for dual biofilms). In general, dual species biofilms demonstrated higher resistance and resilience to GLUT exposure than either of the single species biofilms. P. fluorescens biofilms were more susceptible to the biocide than B. cereus biofilms.

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“…One of the earliest observations related to the different characteristics observed in planktonic and biofilm cells was the increased resistance of cells in biofilms to antimicrobial agents and adverse environmental conditions (Drenkard and Ausubel 2002;McBain et al 2002;Simo˜es et al 2008a;Ferreira et al 2010). However, it is not known why and how bacteria, growing within a biofilm, develop increased resistance to antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Persister cells in a biofilm treated with a biocide

et al. 2011

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This study investigated the physiology and behaviour following treatment with ortho-phthalaldehyde (OPA), of Pseudomonas fluorescens in both the planktonic and sessile states. Steady-state biofilms and planktonic cells were collected from a bioreactor and their extracellular polymeric substances (EPS) were extracted using a method that did not destroy the cells. Cell structure and physiology after EPS extraction were compared in terms of respiratory activity, morphology, cell protein and polysaccharide content, and expression of the outer membrane proteins (OMP). Significant differences were found between the physiological parameters analysed. Planktonic cells were more metabolically active, and contained greater amounts of proteins and polysaccharides than biofilm cells. Moreover, biofilm formation promoted the expression of distinct OMP. Additional experiments were performed with cells after EPS extraction in order to compare the susceptibility of planktonic and biofilm cells to OPA. Cells were completely inactivated after exposure to the biocide (minimum bactericidal concentration, MBC = 0.55 ± 0.20 mM for planktonic cells; MBC = 1.7 ± 0.30 mM for biofilm cells). After treatment, the potential of inactivated cells to recover from antimicrobial exposure was evaluated over time. Planktonic cells remained inactive over 48 h while cells from biofilms recovered 24 h after exposure to OPA, and the number of viable and culturable cells increased over time. The MBC of the recovered biofilm cells after a second exposure to OPA was 0.58 ± 0.40 mM, a concentration similar to the MBC of planktonic cells. This study demonstrates that persister cells may survive in biocide-treated biofilms, even in the absence of EPS.

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Biofouling control using microparticles carrying a biocide

Cited by 31 publications

References 39 publications

The antibacterial and anti-biofouling performance of biogenic silver nanoparticles byLactobacillus fermentum

The antibacterial and anti-biofouling performance of biogenic silver nanoparticles byLactobacillus fermentum

The effects of glutaraldehyde on the control of single and dual biofilms ofBacillus cereusandPseudomonas fluorescens

Persister cells in a biofilm treated with a biocide

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