Microalgal isolates in various habitats have different biocomponents, and isolates found in the swamps of South Sumatra have not been explored deeply. This study was designed to analyse morphologically and isolate microalgae that have potential as biofuel candidates through their lipid content. Results showed that five isolates originating from four different sources in South Sumatra, namely, Chlorella vulgaris Beyerinck (DV 005), Dictyosphaerium granulatum Hindák (DV 003), Nannochloropsis sp. (DV 009), Scenedesmus bernardii G. M. Smith (DV 011) and Golenkinia radiata Chodat (DV 001), were identified. Amongs the five isolates, the C. vulgaris Beyerinck (DV 005) isolate had the highest lipid content (15.30%), indicating its potential as biofuel candidate.