Biofunctional Attributes of Surface Layer Protein and Cell-Bound Exopolysaccharide from Probiotic Limosilactobacillus fermentum (MTCC 5898)

Bhawal, Shalaka; Kumari, Ankita; Kapila, Suman; Kapila, Rajeev

doi:10.1007/s12602-021-09891-4

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…These results are consistent with earlier studies. 74,75 No changes were recorded in red blood cells and hemoglobin levels in stressed or probiotic-ingested mice.…”

Section: Discussionmentioning

confidence: 99%

Probiotic and prebiotic supplementation ameliorates chronic restraint stress-induced male reproductive dysfunction

Akram,

Ali,

Kaul

2023

Food Funct.

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“…These results are consistent with earlier studies. 74,75 No changes were recorded in red blood cells and hemoglobin levels in stressed or probiotic-ingested mice.…”

Section: Discussionmentioning

confidence: 99%

Probiotic and prebiotic supplementation ameliorates chronic restraint stress-induced male reproductive dysfunction

Akram,

Ali,

Kaul

2023

Food Funct.

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“…The outer membranes of bacteria cells consist of a variety of substances, including peptidoglycan, teichoic acid, lipoproteins, lipids, and proteins (Bhawal et al., 2022; Sengupta et al., 2013). The presence of these substances means that bacterial surfaces have a net negative charge and contain a diversity of functional groups, including sulfhydryl, hydroxyl, carboxyl, and amino groups.…”

Section: Single‐cell Encapsulation Technologies For Probioticsmentioning

confidence: 99%

Oral delivery of probiotics using single‐cell encapsulation

Han,

McClements,

Liu

et al. 2024

Comp Rev Food Sci Food Safe

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Adequate intake of live probiotics is beneficial to human health and wellbeing because they can help treat or prevent a variety of health conditions. However, the viability of probiotics is reduced by the harsh environments they experience during passage through the human gastrointestinal tract (GIT). Consequently, the oral delivery of viable probiotics is a significant challenge. Probiotic encapsulation provides a potential solution to this problem. However, the production methods used to create conventional encapsulation technologies often damage probiotics. Moreover, the delivery systems produced often do not have the required physicochemical attributes or robustness for food applications. Single‐cell encapsulation is based on forming a protective coating around a single probiotic cell. These coatings may be biofilms or biopolymer layers designed to protect the probiotic from the harsh gastrointestinal environment, enhance their colonization, and introduce additional beneficial functions. This article reviews the factors affecting the oral delivery of probiotics, analyses the shortcomings of existing encapsulation technologies, and highlights the potential advantages of single‐cell encapsulation. It also reviews the various approaches available for single‐cell encapsulation of probiotics, including their implementation and the characteristics of the delivery systems they produce. In addition, the mechanisms by which single‐cell encapsulation can improve the oral bioavailability and health benefits of probiotics are described. Moreover, the benefits, limitations, and safety issues of probiotic single‐cell encapsulation technology for applications in food and beverages are analyzed. Finally, future directions and potential challenges to the widespread adoption of single‐cell encapsulation of probiotics are highlighted.

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“…1 When present in the host, they perform many beneficial functions, such as competitive elimination of pathogenic bacteria, regulation of the intestinal microbiota, enhancement of epithelial barrier function, and modulation of the immune system. 2 Nevertheless, the key to the successful implementation of these functions is the ability of Lactobacillus to adhere to the epithelial and subepithelial tissues of the host. In different species of Lactobacillus, S-layer proteins (SLPs) have also been shown to be adhesion proteins of epithelial cells and components such as mucus and extracellular matrix proteins 3−5 and they have displayed their potential role in bacterial adhesion, immunobiological, and anti-oxidative functions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Lactobacillus plays a pivotal role in the production of fermented foods and is one of the probiotic groups present in the body of humans and animals . When present in the host, they perform many beneficial functions, such as competitive elimination of pathogenic bacteria, regulation of the intestinal microbiota, enhancement of epithelial barrier function, and modulation of the immune system . Nevertheless, the key to the successful implementation of these functions is the ability of Lactobacillus to adhere to the epithelial and subepithelial tissues of the host.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Domains Responsible for Cell Wall Binding, Self-Assembly, and Adhesion of S-layer Protein from Lactobacillus acidophilus CICC 6074

Kong

Gan

et al. 2022

J. Agric. Food Chem.

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Lactobacillus S-layer protein (SLP) is a biologically active protein on the cell surface. To further elucidate the structures and functions of SLP in Lactobacillus acidophilus CICC 6074, this study was conducted to identify the functional domains of SLP which is responsible for cell wall anchoring, self-assembly, and adhesion. The gene (slpA) of L. acidophilus CICC 6074 SLP was amplified by polymerase chain reaction and speculated functional domains. Fusion proteins of C-terminal truncations from SLP were exogenously expressed in Escherichia coli BL21 (DE3). FITC-labeling N-terminal truncations of SLP were synthesized. The Cterminal domain was more likely to be the binding region, and the cell wall-anchored receptor of SLP was teichoic acid. Furthermore, N-terminal truncations could self-assemble to milk fat globule membrane polar lipid liposomes observed using a fluorescence microscope. Notably, SAN1 (region 32-55) of N-terminal truncations was mainly responsible for the adhesion of SLP to HT-29 cells. These results showed that SLP played a crucial role in the functions of L. acidophilus CICC 6074, which might be of significant reference value for future studies.

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Biofunctional Attributes of Surface Layer Protein and Cell-Bound Exopolysaccharide from Probiotic Limosilactobacillus fermentum (MTCC 5898)

Cited by 10 publications

References 38 publications

Probiotic and prebiotic supplementation ameliorates chronic restraint stress-induced male reproductive dysfunction

Probiotic and prebiotic supplementation ameliorates chronic restraint stress-induced male reproductive dysfunction

Oral delivery of probiotics using single‐cell encapsulation

Identification and Characterization of Domains Responsible for Cell Wall Binding, Self-Assembly, and Adhesion of S-layer Protein from Lactobacillus acidophilus CICC 6074

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