Biofunctional organic nanocrystals for quantitative detection of pathogen deoxyribonucleic acid

Chan, Cangel Pui-yee; Tzang, Lawrence C.H.; Sin, King-keung; Ji, Shenglin; Cheung, Kwan Yee; Tam, Tze-kin; Yang, Mengsu; Renneberg, Reinhard; Seydack, Matthias

doi:10.1016/j.aca.2006.11.025

Cited by 22 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescein diacetate (FDA) was pounded into nanometer‐sized crystals and subsequently adsorbed with streptavidin HPV DNA. The fluorescence signal was directly proportional to the amplicon concentration in the range of 10 3 –10 5 copies/μL .…”

Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

View full text Add to dashboard Cite

Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV‐16 and 18, has been a topic of focus by many researchers. In this systematic review, we highlight the route of development of DNA‐based genosensory detection methods for diagnosis of high risk of HPV precancer. Biosensor detection methods of HPV‐16 and 18 was investigated from 1994 to 2018 using several databases including PubMed, Cochrane Library, Scopus, Google Scholar, SID, and Scientific Information Database. Manual search of references of retrieved articles were also performed. A total of 50 studies were reviewed. By analyzing the most recent developed electrochemical biosensors for the identification of HPV, we observed that the sensor platform fabricated by Wang et al. holds the lowest detection limit reported in the literature for the DNA of HPV‐16. Up to this date, optical, electrochemical, and piezoelectric systems are the main transducers used in the development of biosensors. Among the most sensitive techniques available to study the biorecognition activity of the sensors, we highlight the biosensors based fluorescent, EIS, and QCM. The current systematic review focuses on the sensory diagnostic methods that are being used to detect HPV‐16 and 18 worldwide. Special emphasis is given on the sensory techniques that can diagnosis the individuals with CC. © 2018 BioFactors, 45(2):101–117, 2019

show abstract

Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

View full text Add to dashboard Cite

show abstract

“…For instance, organic and biofunctional nanocrystals were used as labels for quantitative detection of different HPV genotypes (HPV-16, HPV-18, HPV-45) in the range of 10 3 -10 5 copies=L [23]. These streptavidin-coated nano-sized crystals were composed of labels including fluorescein diacetate encapsulated within polyethylene glycol (PEG)-Amine-modified phospholipids.…”

Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

View full text Add to dashboard Cite

| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

show abstract

“…Fluorescein diacetate was ground into a nanocrystals platform, and consequently, they were adsorbed with streptavidin human papillomavirus DNA. The fluorescence signal was proportional to the amplicon concentration in 10 3 –10 5 copies/μl (Chan et al, 2007).…”

Section: Optical Biosensormentioning

confidence: 99%

“….In another study,Chan et al, in 2007, utilized organic nanometer-sized crystals as labels to quantitatively detect different genotypes of HPV including HPV-16, HPV-18, and HPV-45.Fluorescein diacetate was ground into a nanocrystals platform, and consequently, they were adsorbed with streptavidin human papillomavirus DNA. The fluorescence signal was proportional to the amplicon concentration in 10 3 -10 5 copies/μl(Chan et al, 2007).Yue et al, in another project, encoded a combination of various spectral microbeads for multiplex detection of HPV-16 and HPV-18 targets of DNA. A single-layer array of microbeads was advanced with specific DNA of HPV capture probes in a microfluidic structure.…”

mentioning

confidence: 99%

Advances in early diagnosis of cervical cancer based on biosensors

Ghaleh

Shahriary

Izadi

et al. 2022

Biotech & Bioengineering

View full text Add to dashboard Cite

Human papillomavirus (HPV) is a causative agent of cervical cancer among women worldwide. Serological and molecular tests are commonly used to detect and identify HPV, but all the detection methods for HPV have some limitations. Nowadays, considerable advancements in nanosensors have enabled monitoring of hybridization procedures dynamically for HPV detection. Biosensors, as effective, quick, economical, and highly sensitive tools, can be used in the diagnosis of HPV as an alternative technique instead of other detection methods. Biosensor detection methods of HPV in use from 2000 to 2021 were investigated using several databases such as Google Scholar, Scopus, PubMed, and the Scientific Information Database. Furthermore, a manual search of the references of the retrieved articles was performed. On analyzing the most recently developed biosensors for HPV identification, we observed that three biosensor systems, electrochemical, optical, and piezoelectric systems, are the main transducers used in the development of HPV biosensors. The aim of this review is to examine recent research on biosensors for the detection of HPV and perform a comparison with other diagnostic methods.Considering the importance of rapid HPV detection in the control of infection and development of public health measures, improvement of biosensors as an economical and quick method can be very useful in the diagnosis of HPV.

show abstract

Biofunctional organic nanocrystals for quantitative detection of pathogen deoxyribonucleic acid

Abstract: Advances in nanotechnology have had significant impacts on the field of biodiagnostics. In this study, we describe the novel application of dissolvable, organic, and biofunctional nanocrystals for the quantitative detection of a PCR product.

Cited by 22 publications

References 22 publications

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

Advances in early diagnosis of cervical cancer based on biosensors

Contact Info

Product

Resources

About