Biofunctional properties of bioactive peptide fractions from protein isolates of moringa seed (Moringa oleifera)

Abstract

(Moringaceae) is a specie of significant importance because of its multiple nutraceutical properties, that has led to increase in its consumption. The seeds contain a high percentage of protein (37.48%). However, little is known about the bioactive properties of these proteins and peptides, especially those generated by enzymatic hydrolysis. The objective of this study was to evaluate the biofunctional properties of total hydrolysates (TH) and peptide fractions from protein isolates of moringa seeds. Isoelectric protein isolates were prepared and TH were obtained by digestion with trypsin, chymotrypsin and pepsin-trypsin for 2.5 and 5 h. TH were fractioned by ultrafiltration (UF) with a 10 kDa membrane to generate the peptide fractions. In all treatments, the antioxidant capacity was significantly higher in peptide fractions > 10 kDa with 5 h of hydrolysis. The results showed that the fraction > 10 kDa of pepsin-trypsin digested for 5 h presented a better Angiotensin Converting Enzyme inhibition (ACE-I) activity with an IC of 0.224 μg/μl. Also, antidiabetic activity was enhanced in pepsin-trypsin treatment with 5 h of hydrolysis showing an IC of 0.123 μg/μl. Finally, this study showed that hydrolysates of moringa seed proteins had excellent in vitro nutraceutical potential.

A Treatment to Cure Diabetes Using Plant-Based Drug Discovery

“…The enzymes trypsin, chymotrypsin, and pepsin-trypsin are utilised for the first two and a half and five hours, respectively. The use of the pepsin-trypsin digested fraction for hydrolysis intervals of 5 h and 5 h, respectively, for alpha-amylase inhibition was validated, with IC50 values of 0.195 and 0.123 g/ml, respectively, confirming the findings of the previous research [ 59 ]. The IC50 for alcalase hydrolysate was modified and determined to be 0.149 mg/ml for protein hydrolysate of watermelon seeds received with the useful beneficial resource of enzymes together with pepsin, trypsin, and alcalase [ 60 ].…”

First Report on Evaluation of Basic Nutritional and Antioxidant Properties of Moringa Oleifera Lam. from Caribbean Island of Saint Lucia

“…The quantity of determined crude proteins in dry leaves of M. oleifera from South Africa was 30.3% [27]. However, maximum protein content (37.5 g/100 g of DW) was recently reported for M. oleifera seeds cultivated in Mexico [39]. It has been suggested that the protein quantity depends not only on the cultivars and species but also on environmental factors.…”

Moringa oleifera Leaf Supplementation as a Glycemic Control Strategy in Subjects with Prediabetes

“…Its therapeutic value as a cardioprotective, hepatoprotective, neuroprotective, anti-asthmatic, anti-tumor, antimicrobial, hypolipidemic, modulator of intestinal microbiota and antidiabetic agent derives from its phytochemical constituents such as alkaloids and polyphenols [ 4 , 5 , 6 ]. In addition, bioactivity has been proved for peptide fractions (i.e., inhibition of alfa-amylase and angiotensin converting enzyme-1 activities) [ 7 ], isolated polysaccharide fractions [ 8 , 9 ] or the isothiocyanate derivatives of characteristic glucosinolates [ 10 , 11 ].…”