Biofunctionalisation of polymeric scaffolds for neural tissue engineering

Wang, Ting‐Yi; Forsythe, John S.; Parish, Clare L.; Nisbet, David R.

doi:10.1177/0885328212443297

Cited by 42 publications

(17 citation statements)

References 181 publications

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“…In addition, the ability of PCL to support neural cells in vitro and in vivo has demonstrated previously [19][20][21]. However, hydrophobic feature of PCL is known as an adverse feature for cell attachment [22].…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers

et al. 2015

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Cell transplantation strategies have provided potential therapeutic approaches for treatment of neurodegenerative diseases. Mesenchymal stem cells from Wharton's jelly (WJMSCs) are abundant and available adult stem cells with low immunological incompatibility, which could be considered for cell replacement therapy in the future. However, MSC transplantation without any induction or support material causes poor control of cell viability and differentiation. In this study, we investigated the effect of the nanoscaffolds on WJMSCs differentiation into motor neuronal lineages in the presence of retinoic acid (RA) and sonic hedgehog (Shh). Surface properties of scaffolds have been shown to significantly influence cell behaviors such as adhesion, proliferation, and differentiation. Therefore, polycaprolactone (PCL) nanofibers were constructed via electrospinning, surface modified by plasma treatment, and grafted by collagen. Characterization of the scaffolds by means of ATR-FTIR, contact angel, and Bradford proved grafting of the collagen on the surface of the scaffolds. WJMSCs were seeded on nanofibrous and tissue culture plate (TCP) and viability of WJMSCs were measured by MTT assay and then induced to differentiate into motor neuron-like cells for 15 days. Differentiated cells were evaluated morphologically, and real-time PCR and immunocytochemistry methods were done to evaluate expression of motor neuron-like cell markers in mRNA and protein levels. Our results showed that obtained cells could express motor neuron biomarkers at both RNA and protein levels, but the survival and differentiation of WJMSCs into motor neuron-like cells on the PCL/collagen scaffold were higher than cultured cells in the TCP and PCL groups. Taken together, WJMSCs are an attractive stem cell source for inducing into motor neurons in vitro especially when grown on nanostructural scaffolds and PCL/collagen scaffolds can provide a suitable, three-dimensional situation for neuronal survival and differentiation that suggest their potential application towards nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers

et al. 2015

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“…[20][21][22][23] Several biodegradable NGCs using collagen, poly(caprolactone-colactide) (PCLA), etc., have already been approved by the US Food and Drug Administration (FDA) and Conformit Europe (CE) for clinical trial of PNI. 24 Small intestine submucosa (SIS), derived from the submucosal layer of porcine intestine, is an ECM, which consists of more than 90% of the total collagen content of types I and III collagens and several biological factors.…”

mentioning

confidence: 99%

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Shim

Kwon

Lee

et al. 2015

Tissue Engineering Part A

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The present study employed nerve guidance conduits (NGCs) only, which were made of small intestine submucosa (SIS) and poly(caprolactone-co-lactide) (PCLA) to promote nerve regeneration in a peripheral nerve injury (PNI) model with nerve defects of 15 mm. The SIS-and PCLA-NGCs were easily prepared by rolling of a SIS sheet and a bioplotter using PCLA, respectively. The prepared SIS-and PCLA-NGCs fulfilled the general requirement for use as artificial peripheral NGCs such as easy fabrication, reproducibility for mass production, suturability, sterilizability, wettability, and proper mechanical properties to resist collapsing when applied to in vivo implantation. The SIS-and PCLA-NGCs appeared to be well integrated into the host sciatic nerve without causing dislocations and serious inflammation. All NGCs stably maintained their NGC shape for 8 weeks without collapsing, which matched well with the nerve regeneration rate. Staining of the NGCs in the longitudinal direction showed that the regenerated nerves grew successfully from the SIS-and PCLA-NGCs through the sciatic nerve-injured gap and connected from the proximal to distal direction along the NGC axis. SIS-NGCs exhibited a higher nerve regeneration rate than PCLANGCs. Collectively, our results indicate that SIS-and PCLA-NGCs induced nerve regeneration in a PNI model, a finding that has significant implications in the future with regard to the feasibility of clinical nerve regeneration with SIS-and PCLA-NGCs prepared through an easy fabrication method using promising biomaterials.

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“…The diversity and adaptability of biomaterial scaffolds make them an attractive strategy for neural cell replacement therapy (Orive, Anitua, Pedraz, & Emerich, 2009); however, any material used for intracranial delivery should exhibit a number of desirable characteristics. Such materials should (a) be capable of relatively noninvasive delivery; (b) be biomimetic in order to encourage cell survival and host integration; (c) not themselves elicit an exaggerated host immune reaction that can instigate neuroinflammation around the transplantation site; (d) be structurally stable for prolonged periods in situ and where appropriate biodegrade without leaving any undesirable foreign remnants; (e) be modifiable in relation to adhesion molecules, pore size, molecular charge, surface topography and functionalisation; (f) be nontoxic to any cellular components of brain tissue or the encapsulated cells; and (g) be capable of controlled and sustained delivery of therapeutic factors (based on Orive et al (2009) andWang, Forsythe, Parish, andNisbet (2012)).…”

Section: Cellular Brain Repair For Parkinson's Disease -Which Biomamentioning

confidence: 99%

Primary tissue for cellular brain repair in Parkinson's disease: Promise, problems and the potential of biomaterials

Moriarty

Parish

Dowd

2018

Eur J of Neuroscience

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The dopamine precursor, levodopa, remains the "gold standard" treatment for Parkinson's disease, and, although it provides superlative efficacy in the early stages of the disease, its long-term use is limited by the development of severe motor side effects and a significant abating of therapeutic efficacy. Therefore, there remains a major unmet clinical need for the development of effective neuroprotective, neurorestorative or neuroreparatory therapies for this condition. The relatively selective loss of dopaminergic neurons from the nigrostriatal pathway makes Parkinson's disease an ideal candidate for reparative cell therapies, wherein the dopaminergic neurons that are lost in the condition are replaced through direct cell transplantation into the brain. To date, this approach has been developed, validated and clinically assessed using dopamine neuron-rich foetal ventral mesencephalon grafts which have been shown to survive and reinnervate the denervated brain after transplantation, and to restore motor function. However, despite long-term symptomatic relief in some patients, significant limitations, including poor graft survival and the impact this has on the number of foetal donors required, have prevented this therapy being more widely adopted as a restorative approach for Parkinson's disease. Injectable biomaterial scaffolds have the potential to improve the delivery, engraftment and survival of these grafts in the brain through provision of a supportive microenvironment for cell adhesion, growth and immune shielding. This article will briefly review the development of primary cell therapies for brain repair in Parkinson's disease and will consider the emerging literature which highlights the potential of using injectable biomaterial hydrogels in this context.

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Biofunctionalisation of polymeric scaffolds for neural tissue engineering

Cited by 42 publications

References 181 publications

Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers

Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers

Evaluation of Small Intestine Submucosa and Poly(caprolactone-co-lactide) Conduits for Peripheral Nerve Regeneration

Primary tissue for cellular brain repair in Parkinson's disease: Promise, problems and the potential of biomaterials

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