Biogenic silver nanoparticles as an antibacterial agent against bacterial leaf blight causing rice phytopathogen Xanthomonas oryzae pv. oryzae

Namburi, Karunakar Reddy; Kora, Aruna Jyothi; Chetukuri, Anuradha; Kota, Vijaya Sree Meena Kumari

doi:10.1007/s00449-021-02579-7

Cited by 29 publications

(7 citation statements)

References 27 publications

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“…TEM results confirmed that AgNPs did not agglomerate, dispersed well and were almost spherical in shape with particle size in nano range. The size of the AgNPs obtained in the current study was matched with earlier reports on rice leaf extract mediated AgNPs synthesis 45 , 46 .…”

Section: Resultssupporting

confidence: 90%

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Inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (Oryza sativa)

Chowdhury,

Kumar,

Mahanty

et al. 2024

Sci Rep

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Rice (Oryza sativa) being among the most important food crops in the world is also susceptible to various bacterial and fungal diseases that are the major stumbling blocks in the way of increased production and productivity. The bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae and the sheath blight disease caused by Rhizoctonia solani are among the most devastating diseases of the rice crop. In spite of the availability of array of chemical control, there are chances of development of resistance. Thus, there is a need for the nanotechnological intervention for management of disease in the form of copper and silver nano-composites. The copper (CuNPs) and silver nanoparticles (AgNPs) were synthesized using green route and characterized using different high throughput techniques, i.e., UV–Vis, FT-IR, DLS, XRD, FE-SEM, TEM. The particle size and zeta potential of synthesized CuNPs and AgNPs were found 273 nm and − 24.2 mV; 95.19 nm and − 25.5 mV respectively. The nanocomposite of CuNPs and AgNPs were prepared having particle size in the range of 375–306 nm with improved stability (zeta potential − 54.7 to − 39.4 mV). The copper and silver nanoparticle composites evaluated against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani were found to have higher antibacterial (inhibition zone 13 mm) and antifungal activities (77%) compared to only the copper nanoparticle (8 mm; 62% respectively). Net house trials of nano-composite formulations against the bacterial blight of rice also corroborated the potential of nanocomposite formulation. In silico studies were carried out selecting two disease-causing proteins, peptide deformylase (Xanthomonas oryzae) and pectate lyase (Rhizoctonia solani) to perform the molecular docking. Interaction studies indicatedthat both of these proteins generated better complex with CuNPs than AgNPs. The study suggested that the copper and silver nano-composites could be used for developing formulations to control these devastating rice diseases.

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Section: Resultssupporting

confidence: 90%

“…The formation of silver nanoparticle was confirmed by the appearance of yellow–brown color in the reaction mixture. These findings are in tune with earlier leaf extract based AgNPs syntheses carried out in different leaf extract 45 , 46 , 54 , 55 .…”

Section: Discussionsupporting

confidence: 90%

Inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (Oryza sativa)

Chowdhury,

Kumar,

Mahanty

et al. 2024

Sci Rep

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“…For the identification of the structure of silver and silica of the nanoparticles, XRD spectra were obtained. By comparing the obtained spectra with bibliographic data, it was confirmed that the structure of silver is a face-centered cubic (fcc) and coating presence of silica is detected by characteristic silica peaks. − In detail, Ag(111), Ag(220), and Ag(311) planes are identified in all samples as well as in silica cristobalite, which confirms the silica coating of the nanoparticles , (Figure ).…”

Section: Resultsmentioning

confidence: 58%

Novel Hybrid Silver–Silica Nanoparticles Synthesized by Modifications of the Sol–Gel Method and Their Theranostic Potential in Cancer

Nikolopoulou,

Kalska,

Basa

et al. 2023

ACS Appl. Bio Mater.

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Cancer is one of the leading causes of death worldwide. Conventional therapies lack selectivity and suffer from toxicity and drug resistance, leading to metastasis. To overcome these limitations, a new category of nanomaterials exploiting the tumor characteristics has been developed in cancer nanotherapeutics. Among them, pH, metabolism, and the disrupted architecture of cells can be exploited for theranostic applications. Such nanomaterials can be inorganic nanoparticles with silver ones and gain high attention as diagnostic, therapeutic, and antibacterial compounds. Silver has been linked with triggering the death of cancer cells via DNA damage due to the production of reactive oxygen species (ROS) during photodynamic therapy. Thus, improvement of biocompatibility, modification with targeted agents, and drug conjugation promote the use of silver nanoparticles. In this work, we managed to synthesize hybrid Ag@SiO 2 core−shell nanoparticles via a modified sol−gel method by tackling the known etching of silver caused by ammonia by employing different bases of the sol−gel reaction. The bases used in the synthetic route were diethylamine (DEA) and triethylamine (TEA) and were monitored with silver nanoparticles individually from the absorbance peak of silver in the UV−vis region, showing no etching of silver in contrast with ammonia, which is usually used in the sol−gel method. Furthermore, we synthesized biocompatible nanoparticles with anticancer and diagnostic properties toward breast cancer cells and glioblastoma cells. The nanoparticles were characterized both structurally and morphologically. Their biological evaluation suggests minor toxicity toward healthy cells and red blood cells (RBCs). Also, the diagnostic potential of the hybrid nanoparticles was exploited by optical fluorescence microscopy. Therefore, we strongly suggest the investigation of such nanostructures as a dual platform for the diagnosis and therapy of cancer.

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“…As a result, research into employing nanotechnology to improve medication delivery has begun ( Hawar et al, 2022 ). There are many studies using plant sources (leaf, flower, root, fruit, or whole plant) to obtain silver nanoparticles (AgNPs) with biological approaches ( Namburi et al, 2021 ). AgNPs obtained in synthesis studies with plant sources do not require special conditions and are synthesized with an eco-friendly approach, as well as the fact that the synthesis process is easy, and cheap and the amount of product is higher, among the factors that increase the interest in biological approaches ( Rather et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

Keskin,

Ölçekçi,

Baran

et al. 2023

Front. Chem.

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The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the D. kaki L. (DK); silver nanoparticles were synthesized in a short time and simply with an eco-friendly approach. The properties of the synthesized silver nanoparticles (AgNPs) were characterized using various techniques and measurements. In the characterization data of AgNPs, Maximum absorbance at 453.34 nm wavelengths, the average size distribution of 27.12 nm, the surface charge of −22.4 mV, and spherical appearance were observed. LC-ESI-MS/MS analysis was used to assess the compound composition of D. kaki leaf extract. The chemical profiling of the crude extract of D. kaki leaves revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the identification of five major high-feature compounds: two major phenolic acids (Chlorogenic acid and Cynarin), and tree flavonol glucosides (hyperoside, quercetin-3-glucoside, and quercetin-3- D-xyloside). The components with the highest concentrations were cynarin, chlorogenic acid, quercetin-3- D-xyloside, hyperoside, and quercetin-3-glucoside, respectively. Antimicrobial results were determined by a MIC assay. The biosynthesized AgNPs exhibited strong antibacterial activity against the human and food pathogen Gram (+ and −) bacteria and good antifungal activity against pathogenic yeast. It was determined that 0.03–0.050 μg/mL concentrations ranges of DK-AgNPs were growth suppressive concentrations on all pathogen microorganisms. The MTT technique was used to study the cytotoxic effects of produced AgNPs on cancer cell lines (Glioblastoma (U118), Human Colorectal Adenocarcinoma (Caco-2), Human Ovarian Sarcoma (Skov-3) cancer cell lines, and Human Dermal Fibroblast (HDF) healthy cell line). It has been observed that they have a suppressive effect on the proliferation of cancerous cell lines. After 48 h of treatment with Ag-NPs, the DK-AgNPs were found to be extremely cytotoxic to the CaCo-2 cell line, inhibiting cell viability by up to 59.49% at a concentration of 50 g mL−1. It was found that the viability was inversely related to the DK-AgNP concentration. The biosynthesized AgNPs had dose-dependent anticancer efficacy. Because of the high concentration of bioactive chemicals in Diospyros kaki, it may be employed as a biological resource in medicinal applications. DK-AgNPs were shown to be an effective antibacterial agent as well as a prospective anticancer agent. The results provide a potential approach for the biogenic production of DK-AgNPs utilizing D. kaki aqueous leaf extract.

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Biogenic silver nanoparticles as an antibacterial agent against bacterial leaf blight causing rice phytopathogen Xanthomonas oryzae pv. oryzae

Cited by 29 publications

References 27 publications

Inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (Oryza sativa)

Inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (Oryza sativa)

Novel Hybrid Silver–Silica Nanoparticles Synthesized by Modifications of the Sol–Gel Method and Their Theranostic Potential in Cancer

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

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