Biogenic synthesis of Marsilea quadrifolia gold nanoparticles: a study of improved glucose utilization efficiency on 3T3-L1 adipocytes

Chowdhury, Anindita; Kunjiappan, Selvaraj; Bhattacharjee, Chiranjib; Somasundaram, Balasubramanian; Panneerselvam, Theivendren

doi:10.1007/s11626-017-0136-3

Cited by 23 publications

(16 citation statements)

References 40 publications

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“…Moreover, the high dose of AuNPs (30 µg/ml) and pioglitazone showed a similar effect. Polyphenols and flavonoids of the extract attached to the surface of AuNPs improved bioavailability, induced transmitted GLUT4 vesicle to the cell membrane and probably increased glucose uptake in adipocytes (Chowdhury et al, 2017).…”

Section: Diabetes Mellitusmentioning

confidence: 96%

Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review

Nouri

Hajialyani

Izadi

et al. 2020

Front. Bioeng. Biotechnol.

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Metabolic syndrome includes a series of metabolic abnormalities that leads to diabetes mellitus and cardiovascular diseases. Plant extracts, due to their unique advantages like anti-inflammatory, antioxidant, and insulin sensitizing properties, are interesting therapeutic options to manage MetS; however, the poor solubility and low bioavailability of lipophilic bioactive components in the herbal extracts are two critical challenges. Nano-scale delivery systems are suitable to improve delivery of herbal extracts. This review, for the first time, focuses on nanoformulations of herbal extracts in MetS and related complications. Included studies showed that several forms of nano drug delivery systems such as nanoemulsions, solid lipid nanoparticles, nanobiocomposites, and green-synthesized silver, gold, and zinc oxide nanoparticles have been developed using herbal extracts. It was shown that the method of preparation and related parameters such as temperature and type of polymer are important factors affecting physicochemical stability and therapeutic activity of the final product. Many of these formulations could successfully decrease the lipid profile, inflammation, oxidative damage, and insulin resistance in in vitro and in vivo models of MetS-related complications. Further studies are still needed to confirm the safety and efficacy of these novel herbal formulations for clinical application.

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Section: Diabetes Mellitusmentioning

confidence: 96%

Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review

Nouri

Hajialyani

Izadi

et al. 2020

Front. Bioeng. Biotechnol.

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“…52 The activation of AMPK could suppress hepatic glucogensis and fatty acid oxidation through Metformin-a gold standard drug by glucose uptake via GLUT4 translocation in skeletal muscles. 35 Further, GLUT4 catalyzes the glucose transport across the cell membranes through ATP-independent pathway and facilitative diffusion mechanism. This is so as long as the concentration of insulin is low.…”

Section: In Vitro Cellular Viability Studiesmentioning

confidence: 99%

“…When the insulin binds to a surface receptor (IRS) on adipocytes, initiates activation of the phosphatidylinositol 3-kinase (PI3K) pathway, resulting in muscles triggers the cascade of events that culminates in GLUT4 translocation from the vesicle to the plasma membrane. 35 Further, GLUT4 catalyzes the glucose transport across the cell membranes through ATP-independent pathway and facilitative diffusion mechanism. 54 Many synthetic compounds have been reported for upregulating the insulin-dependent pathway and enhancing glucose translocation.…”

Section: In Vitro Glucose Uptake Efficiency Of Indoloquinoxaline Dementioning

confidence: 99%

“…,35 Briefly, the differentiated 3T3-L1 adipocytes (6 × 10 5 cells/well), were cultured in 24-well plate with insulin and serum starved DMEM for 16 hr, and the cells were then washed three times with phosphate buffered saline (pH 7.4).Subsequently, cells were incubated with 200 μl of differentiation medium containing various amounts of NPs (1, 5, 15, 30, 40 μg/well), standard drugs such as Pioglitazone (10 μM/well), human insulin (10 μM/well), Metformin (10 μM/well), and Indoloquinoxaline (40 μg/well) for 24 hr. Glucose uptake was started by the addition of 100 μl PBS containing 20 μM of 6-NBDG for 30 min.…”

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confidence: 99%

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Design and in silico modeling of Indoloquinoxaline incorporated keratin nanoparticles for modulation of glucose metabolism in 3T3‐L1 adipocytes

Kunjiappan

Panneerselvam²,

Pavadai

et al. 2019

Biotechnology Progress

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The following study was done to assess the glucose utilizing efficiency of Indoloquinoxaline derivative incorporated keratin nanoparticles (NPs) in 3T3-L1 adipocytes. Indoloquinoxaline derivative had wide range of biological activities including antidiabetic activity. In this view, Indoloquinoxaline moiety containing N,methanamine compound was designed and synthesized, and further it is incorporated into keratin nanoparticles. The formulated NPs, drug entrapment efficiency, releasing capacity, stability, and physicochemical properties were characterized by various spectral analyzer and obtained results of characterizations were confirmed the properties of NPs. The analysis of mechanism underlying the glucose utilization of NPs was examined through molecular docking with identified target, and observed in silico study reports shown strong interaction of NPs in the binding pockets of AMPK and PTP1B. Based on the in silico screening, the formulated NPs was performed for in vitro cellular viability and glucose uptake studies on 3T3-L1 adipocytes. Interestingly, 40 μg of NPs displayed 78.2 ± 2.76% cellular viability, and no cell death was observed at lower concentrations. Further, the concentration dependent glucose utilization was observed at different concentrations of NPs in 3T3-L1 adipocytes. The results of NPs (40 μg) on glucose utilization have revealed eminent result 58.56 ± 4.54% compared to that of Metformin (10 μM) and Insulin (10 μM). The identified results clearly indicated that Indoloquinoxaline derivative incorporated keratin NPs significantly increased glucose utilization efficiency and protect the cells against the insulin resistance. K E Y W O R D S 3T3-L1 adipocytes, glucose uptake, in silico modeling, Indoloquinoxaline, keratin nanoparticles

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“…The aerial part of M. quadrifolia extract using chloroform and ethyl acetate has shown proven antibacterial, cytotoxic, and antioxidant effect [10]. Moreover, methanolic extract of M. quadrifolia fern has been reported to the higher efficiency of glucose utilization and cellular viability in 3T3-L1 adipocytes with lower toxicity [11]. Thus, in the current investigation, the gold nanoparticles (AuNPs) were synthesized by employing the aqueous leaf extract of M. quadrifolia as a reducing agent.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxicity and Antioxidant Evaluation of Biogenic Synthesized Gold Nanoparticles From Marsilea Quadrifolia on Lung and Ovarian Cancer Cells

Balashanmugam

Christas²,

Kowsalya³

2018

Int J App Pharm

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Objective: The biogenic gold nanoparticles are considered to be extremely impressive for its wide range of applications in pharmaceutics and therapeutics. The present study was aimed at the biogenic synthesis of gold nanoparticles (AuNPs) from Marsilea quadrifolia aqueous extract and to investigate its antioxidant property and cytotoxic effect on human ovarian teratocarcinoma (PA-1) and lung adenocarcinoma (A549) cell lines.Methods: The biogenic AuNPs was synthesized using an aqueous extract of Marsilea quadrifolia. The synthesized biogenic AuNPs were characterized by ultraviolet (UV) visible spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). The biogenic AuNPs was assessed for its stability over a period of time and antioxidant activity. The cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The synthesized biogenic AuNPs showed peculiar ruby red color and a surface plasmon resonance (SPR) peak at 544 nm in the UV-Vis spectrum. The characterization of biogenic AuNPs by TEM, EDX and XRD revealed well dispersed spherical particles ranging from 10-40 nm and the presence of elemental gold and its crystalline nature, respectively. The AuNPs showed good stability and the scavenging activity at 50 μg/ml. The in vitro cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines recorded half maximal inhibitory concentration (IC50) of 45.88 μg/ml and 52.015 μg/ml, respectively.Conclusion: The biogenic AuNPs demonstrated superior antioxidant and antiproliferative activities against cancer cell lines.

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Biogenic synthesis of Marsilea quadrifolia gold nanoparticles: a study of improved glucose utilization efficiency on 3T3-L1 adipocytes

Cited by 23 publications

References 40 publications

Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review

Nanophytomedicines for the Prevention of Metabolic Syndrome: A Pharmacological and Biopharmaceutical Review

Design and in silico modeling of Indoloquinoxaline incorporated keratin nanoparticles for modulation of glucose metabolism in 3T3‐L1 adipocytes

In Vitro Cytotoxicity and Antioxidant Evaluation of Biogenic Synthesized Gold Nanoparticles From Marsilea Quadrifolia on Lung and Ovarian Cancer Cells

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