Degenerative diseases are results of deterioration of cells and tissues with aging either by unhealthy lifestyle or normal senescence. The degenerative disease likely affects central nervous system and cardiovascular system to a great extent. Certain medications and therapies have emerged for the treatment of degenerative diseases, but in most cases bearing with poor solubility, lower bioavailability, drug resistance, and incapability to cross the bloodbrain barrier (BBB). Hence, it has to be overcome with conventional treatment system; in this connection, nanotechnology has gained a great deal of interest in recent years. Moreover, nanotechnology and nanocarrier-based approach drug delivery system could revolutionize the treatment of degenerative diseases by faster absorption of drug, targeted interaction at specific site, and its release in a controlled manner into human body with minimal side effects. The core objective of this review is to customize and formulate therapeutically active molecules with specific site of action and without affecting other organs and tissues to obtain effective result in the improvement of quality of health. In addition, the review provides a concise insight into the recent developments and applications of nanotech and nanocarrier-based drug delivery for the treatment of various degenerative diseases.

No abstract

This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morphological characteristics were analyzed by XRD, TG-DTA, SEM-EDS, HRTEM, and SAED. The results of characterization studies were used to assess the properties of GNPs. The in vitro cytotoxicity screening indicates that 100 μM of biogenic GNPs were displayed 71.23 ± 1.56% of cellular viability in 3T3-L1 adipocyte cells. Subsequently, increased glucose utilization of biosynthesized GNPs based on a dose-dependent manner on 3T3-L1 has also been demonstrated. The effect of GNPs (30 μg) on glucose uptake was higher than that of insulin and metformin. Moreover, the observed results clearly highlight that the biogenic GNPs have higher efficiency of glucose utilization and cellular viability in 3T3-L1 adipocytes with lower toxicity.

A noninvasive intracellular component analysis technique is important in cancer treatment and the initial identification of cancer. Carbon nanomaterials/nanostructures, such as carbon nanotubes and graphene, have little to no surface enhanced Raman scattering (SERS) ability. Because of these structures' low Raman responses, they are conjugated with gold or silver to attain the SERS-active ability to detect normal fibroblasts and HeLa cancer cells. To the best of our knowledge, the effectiveness of the individual use of carbon nanomaterials as a nonplasmonic SERS-active platform for in vitro cancer/normal cell detection has not been investigated to date. Here, for the first time, we introduce a unique nonplasmonic SERS-based biosensing platform that uses a biocompatible self-assembled threedimensional interconnected nanocarbon web (INW) for in vitro detection and differentiation of HeLa cells and fibroblasts. The sub-10-nm morphology of the INW facilitates the endocytic uptake of INW clusters to the cells, and its SERS functionality introduces live cell Raman sensing. The INW platform has achieved an enhancement factor (EF) of 3.66 × 10 4 and 9.10 × 10 3 with crystal violet and Rhodamine 6G dyes, respectively, significant in comparison to the EF of graphene surfaces (2−17). The results of the time-based Raman spectroscopy of live HeLa cells and fibroblasts revealed chemical fingerprints of intracellular components, such as DNA/RNA, proteins, and lipids. The components' spectroscopic differences facilitate and elucidate the specification of each cell. The highest Raman enhancement achieved was fourfold for fibroblasts (protein) and sixfold for HeLa cells (DNA). Furthermore, the SERS spectra along with scanning electron microscopy and fluorescence microscopy analysis of the immobilized cells after 24 and 48 h shed light on the health of fibroblasts and HeLa cells. A photon energy-induced ionization achieved with a femtosecond laser fabricated a biocompatible INW platform with the designated unique attributes. This simple, label-free, in vitro diagnosis approach for HeLa cells and fibroblasts has strong potential for cancer research.

No abstract