Biogenic synthesis of multifunctional silver nanoparticles from Rhodotorula glutinis and Rhodotorula mucilaginosa: antifungal, catalytic and cytotoxicity activities

Abstract

Silver nanoparticles (AgNPs) have several technological applications and may be synthetized by chemical, physical and biological methods. Biosynthesis using fungi has a wide enzymatic range and it is easy to handle. However, there are few reports of yeasts with biosynthetic ability to produce stable AgNPs. The purpose of this study was to isolate and identify soil yeasts (Rhodotorula glutinis and Rhodotorula mucilaginosa). After this step, the yeasts were used to obtain AgNPs with catalytic and antifungal activity evaluation. Silver Nanoparticles were characterized by UV-Vis, DLS, FTIR, XRD, EDX, SEM, TEM and AFM. The AgNPs produced by R. glutinis and R. mucilaginosa have 15.45 ± 7.94 nm and 13.70 ± 8.21 nm (average ± SD), respectively, when analyzed by TEM. AgNPs showed high catalytic capacity in the degradation of 4-nitrophenol and methylene blue. In addition, AgNPs showed high antifungal activity against Candida parapsilosis and increase the activity of fluconazole (42.2% for R. glutinis and 29.7% for R. mucilaginosa), while the cytotoxicity of AgNPs was only observed at high concentrations. Finally, two yeasts with the ability to produce AgNPs were described and these particles showed multifunctionality and can represent a technological alternative in many different areas with potential applications.

A review on the biosynthesis of metal and metal salt nanoparticles by microbes

“…Table 2 shows the list of various yeasts used for the synthesis of different metal nanoparticles. [67][68][69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84]…”

Microbial Nano-Factories: Synthesis and Biomedical Applications

“…Yeasts cells are particularly well-known for their ability to synthesize semiconductor nanoparticles, particularly that of cadmium sulfide (CdS). There are reports on the production of other metal nanoparticles, particularly AgNPs, by yeasts, including Pichia capsulata (Subramanian et al, 2010), Candida guilliermondii (Mishra et al, 2011), Saccharomyces boulardii (Kaler et al, 2013), Kluyveromyces marxianus (Ashour, 2014), Candida utilis (Waghmare et al, 2015), Candida lusitaniae (Eugenio et al, 2016), Saccharomyces cerevisiae (Sowbarnika et al, 2018), Candida glabrata (Jalal et al, 2018), Candida albicans (Ananthi et al, 2018), Rhodotorula glutinis, and Rhodotorula mucilaginosa (Cunha et al, 2018). The silver-tolerant yeast strain MKY3 was used for the production of silver nanoparticles (Kowshik et al, 2002).…”

The Emerging Trend of Bio-Engineering Approaches for Microbial Nanomaterial Synthesis and Its Applications

“…The proteins present in the fungal extract get adsorbed on the surface, thereby enhancing the colloidal stability of the Ag NPs (Du et al, 2015). Ag NPs synthesized by R. glutinis and Rhodotorula mucilaginosa were found effective in degrading the highly toxic chemical pollutants such as methylene blue (MB) and 4-nitrophenol (4-NP; Vidhu and Philip, 2014;Cunha et al, 2018).…”