Biogenic synthesis of zinc oxide nanoparticles using Ruta graveolens (L.) and their antibacterial and antioxidant activities

Abstract

In the present investigation, green synthesis of zinc oxide nanoparticles were successfully synthesized by biological method using aqueous stem extract of Ruta graveolens act as reducing agent. Formation of ZnO nanoparticles were characterized by powder X-ray diffraction (PXRD), UV-visible spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Zinc oxide nanoparticles were subjected to biological properties such as antibacterial and antioxidant studies. The PXRD pattern reveals that ZnO sample belongs to hexagonal phase with Wurtzite structure. The UV-vis absorption spectrum shows an absorption band at 355 nm due to ZnO nanoparticles. SEM images show that the particles have spherical like structure with large surface area and the average crystallite sizes were found to be in the range *28 nm. These observations were confirmed by TEM analysis. The ZnO nanoparticles are found to inhibit the antioxidant activity of 1,1-diphenyl-2-picrylhydrazyl free radicals effectively. ZnO Nps exhibit significant bactericidal activity against Gram -ve bacterial strains such as Klebsiella aerogenes, Pseudomonas aeruginosa, Escherichia coli and Gram ?ve Staphylococcus aureus by agar well diffusion method.

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

“…The spectra of the non-annealed nano powder displayed an amorphous signature. The annealed nano powder showed peaks that can be indexed to the hexagonal wurtzite structure ZnO, which coincide with the standard JCPDS card (202) plane [37]. The strong sharp diffraction peak indicate that ZnO nanoparticles annealed at 500°C are well crystallized [6,31].…”

“…Lastly the peak at 300°C is due to the formation of Zinc Oxide nanoparticles and decomposition of organic matter [35,36] High Resolution Transmission Electron Microscope (HRTEM) Figure 3 reports a typical High-Resolution Transmission Electron Microscope (HRTEM), Energy Dispersive X-Ray (EDX) as well as Selected Area Electron Diffraction (SAED) of ZnO nanoparticles annealed at 500°C in air. Figure 3a shows that the particles were highly agglomerated quasi-spherical in shape [37]. The average diameter of the nanoparticles was found to be of the order of (f particles ) ≈ 28.09 ± 5 nm.…”

Synthesis of Nα-protected formamides from amino acids using MgO nano catalyst: Study of molecular docking and antibacterial activity

“…Antibacterial activity of synthesized formamides was screened by agar well di usion method [51,52] against pathogenic bacterial strains such as G ve bacteria Escherichia coli (NCIM-5051) and G +ve bacteria Staphylococcus aureus (NCIM-5022). The nutrient medium was prepared by dissolving 37.0 g of nutrient nutrient agar medium was poured into petri-dishes and allowed to solidify.…”

Green Synthesis and Characterization of Marine Yeast-Mediated Silver and Zinc Oxide Nanoparticles and Assessment of Their Antioxidant Activity

“…On the other hand, in a recent study, they have observed DPPH scavenging activity of synthesized silver nanoparticles extracted from leaf samples and they have found highest activity as 4.30 µg/ml [34]. Similarly, in Lingaraju et al [35], synthesized ZnO nanoparticles showed good scavenging activity with inhibitory concentration (IC 50 ) value of 9.34 mg/ml. In a recent study, they have also got the similar results [36].…”