Biogeography of Vaucheria species from European freshwater/soil habitats: implications from chloroplast genomes

PHYLOGENETIC ANALYSIS OF 32 STRAINS OFVAUCHERIA(XANTHOPHYCEAE) USING THErbcL GENE AND ITS TWO FLANKING SPACER REGIONS1

“…Entwisle (1988b) also combined V. prona and V. hamata , and our results show no decisive reason to separate these two species. With respect to the V. bursata clade, we found two strains (CCMP1084, UTEX2067) that occupied a well‐supported branch that was sister to the remaining taxa, and perhaps these correspond to the two major groups identified by Linne von Berg and Kowallik (1996). However, within our second “bursata” clade were V. dilwynii and V. repens , and the scrobiculate walls of V. dilwynii were unmistakable.…”

“…Other nuclear-encoded genes may prove more valuable. Nevertheless, the identical sequences for two strains of V. prona suggest, as does the work by Linne von Berg and Kowallik (1996), the existence of genetically identical organisms from different geographic regions. Because of the geographic distance between the collection sites of the two strains, there must be some genuine retention of genetic information over time and space.…”

“…Rieth 1980. Two studies, one emphasizing morphometric features (Entwisle 1988b) and one emphasizing RFLP analysis of chloroplast DNA (Linne von Berg and Kowallik 1996), provide good examples. The length and diameter of the gametophore peduncle, vegetative siphon diameter, oogonial pedicle length, antheridial curvature, oogonial length, and number of oogonia were not found to be of diagnostic value for members of the section Racemosae or the V. frigida / V. prona complex (Entwisle 1988b).…”

“…Conversely, Entwisle found that thickness and texture of the oogonial wall, the oogonial shape, and the gametangial orientation were useful in distinguishing V. frigida (including V. terrestris ) from V. prona. Using molecular techniques, Linne von Berg and Kowallik (1996) found that, for 18 strains of V. bursata (section Corniculatae), the Eco RI restriction fragment patterns could be arranged into two major assemblages (seven strains, eight strains), with three strains having little in common with the two assemblages or each other. Similarly, examination of strains of V. cruciata (section Anomalae), V. geminata (section Racemosae sensu Entwisle), and V. prolifera (section Globiferae) revealed identical patterns, similar patterns, and unrelated patterns within each section (Linne von Berg and Kowallik 1996).…”

“…We used different strains and did not conduct statistical morphometic analyses or chloroplast RFLP analyses, so we cannot make direct comparisons to the previous studies (Entwisle 1988b, Linne von Berg and Kowallik 1996). However, indirect comparisons are nonetheless interesting.…”