Bioguided Fractionation ShowsCassia alataExtract to InhibitStaphylococcus epidermidisandPseudomonas aeruginosaGrowth and Biofilm Formation

Saito, Samuel Takashi; Trentin, Danielle da Silva; Macêdo, Alexandre José; Pungartnik, Cristina; Gosmann, Grace; Silveira, Jaqueline de Deos; Guecheva, Temenouga Nikolova; Henriques, João Antônio Pêgas; Brendel, Martin

doi:10.1155/2012/867103

Cited by 30 publications

(24 citation statements)

References 35 publications

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“…This compound could be either the same one responsible for the observed antimicrobial effect at lower doses (induction only at CHE doses >5 mg/mL) or another non-toxic compound of CHE that induces these changes in the bacterial metabolism. Similar observations were already reported by Saito et al (2012) for the same bacterium, i.e., when testing antimicrobial activity of a plant extract (Cassia alata) in P. aeruginosa. …”

Section: Resultssupporting

confidence: 89%

Antimicrobial activity of fermented Theobroma cacao pod husk extract

et al. 2014

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ABSTRACT. Theobroma cacao L. contains more than 500 different chemical compounds some of which have been traditionally used for their antioxidant, anti-carcinogenic, immunomodulatory, vasodilatory, analgesic, and antimicrobial activities. Spontaneous aerobic fermentation of cacao husks yields a crude husk extract (CHE) with antimicrobial activity. CHE was fractioned by solvent partition with polar solvent extraction or by silica gel chromatography and a total of 12 subfractions were analyzed for chemical composition and bioactivity. CHE was effective against the yeast Saccharomyces cerevisiae and the basidiomycete Moniliophthora perniciosa. Antibacterial activity was determined using 6 strains: Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus subtilis (Gram-positive) and Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella choleraesuis (Gram-negative). At doses up to 10 mg/mL, CHE was not effective against the Gram-positive bacteria tested but against medically important P. aeruginosa and S. choleraesuis with a minimum inhibitory concentration (MIC) of 5.0 mg/mL. Sub-fractions varied widely in activity and strongest antibacterial activity was seen with CHE8 against S. choleraesuis (MIC of 1.0 mg/mL) and CHE9 against S. epidermidis (MIC of 2.5 mg/mL). All bioactive CHE fractions contained phenols, steroids, or terpenes, but no saponins. Fraction CHE9 contained flavonoids, phenolics, steroids, and terpenes, amino acids, and alkaloids, while CHE12 had the same compounds but lacked flavonoids.

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Section: Resultssupporting

confidence: 89%

Antimicrobial activity of fermented Theobroma cacao pod husk extract

et al. 2014

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“…These findings indicate an increasing need for more effective antimicrobial agents with lower costs [19]. …”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effect of the Triterpene 3β,6β,16β-Trihydroxylup-20(29)-ene on Planktonic Cells and Biofilms from Gram Positive and Gram Negative Bacteria

Evaristo

Albuquerque

Santos

et al. 2014

BioMed Research International

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This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a triterpene isolated from Combretum leprosum Mart., in inhibiting the planktonic growth and biofilms of Gram positive bacteria Streptococcus mutans and S. mitis. The antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm potential was determined by quantifying total biomass and enumerating biofilm-entrapped viable bacteria. In addition, the acute toxicity of CLF1 on Artemia sp. nauplii was also determined. The results showed that CLF1 was able in inhibiting the growth of S. mutans and S. mitis with MIC and MBC of 7.8 μg/mL and 15.6 μg/mL, respectively. CLF1 was highly effective on biofilms of both bacteria. Only 7.8 μg/mL CLF1 was enough to inhibit by 97% and 90% biomass production of S. mutans and S. mitis, respectively. On the other hand, such effects were not evident on Gram negative Pseudomonas aeruginosa and Klebsiella oxytoca. The toxicity tests showed that the LC50 of CLF1 was 98.19 μg/mL. Therefore, CLF1 isolated from C. leprosum may constitute an important natural agent for the development of new therapies for caries and other infectious diseases caused by S. mutans and S. mitis.

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“…Ca. alata leaves are a rich source of flavonoids and these include compounds such as kaempferol, quercetin and their glycosides having diverse biological activities (Saito et al 2012). Among these, quercetin and kaempferol have been reported for the QS inhibitory activity against C. violaceum ATCC 12472 (Vasavi et al 2014).…”

Section: Phytocompounds In F-af and Their Qsi Activitymentioning

confidence: 99%

A medicinal herbCassia alataattenuates quorum sensing inChromobacterium violaceumandPseudomonas aeruginosa

Rekha

Vasavi

Vipin

et al. 2017

Lett Appl Microbiol

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Present study demonstrates the quorum sensing inhibitory activity of metabolites from Cassia alata, an important medicinal herb which is commonly used worldwide in the treatment of infections caused by microorganisms. An extract prepared from the leaves of the plant showed activity against quorum sensing in Chromobacterium violaceum and was also effective against attenuating the quorum sensing controlled virulence factors in Pseudomonas aeruginosa. Activity is attributed to the rich flavonoid composition of the plant. Results of the present investigation throw an insight into the possibility of developing drug formulations using the isolated compounds against infections caused by quorum sensing-mediated pathogenicity of bacteria.

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Bioguided Fractionation ShowsCassia alataExtract to InhibitStaphylococcus epidermidisandPseudomonas aeruginosaGrowth and Biofilm Formation

Cited by 30 publications

References 35 publications

Antimicrobial activity of fermented Theobroma cacao pod husk extract

Antimicrobial activity of fermented Theobroma cacao pod husk extract

Antimicrobial Effect of the Triterpene 3β,6β,16β-Trihydroxylup-20(29)-ene on Planktonic Cells and Biofilms from Gram Positive and Gram Negative Bacteria

A medicinal herbCassia alataattenuates quorum sensing inChromobacterium violaceumandPseudomonas aeruginosa

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