Bioguided isolation of compounds with antioxidant activity to improve the in vitro maturation of mammalian oocytes

Oliveira, Lhara Ricarliany Medeiros de; Santos, Maria Valéria de Oliveira; Bertini, Luciana Medeiros; Pereira, Alexsandra Fernandes

doi:10.33448/rsd-v9i8.5137

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…Oocyte maturation induction depends on the successful conclusion and integration of a number of important processes related to both the nuclear and cytoplasmic components of maturation (Moor et al,1998). Meiotic development to metaphase II (MII) can be accelerated using luteinizing hormones, which improved the original in vitro maturation procedure (de Oliveira et al 2020).…”

Section: Resultsmentioning

confidence: 99%

Seasonal Influence on Oocyte Recovery Rate, Quality and in vitro Maturation in Cows

Ammari¹,

Alhimaidi²,

Amran³

et al. 2023

IJAR

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Background: Recent breakthroughs in vitro maturation, fertilization and culture technology have allowed for an increase in the number of offspring generated from genetically better females, but this progress is still hampered by critical factors affecting oocyte yield and quality. This study aims to evaluate the effect of the seasons on the rate of maturation and recovery of oocytes. Methods: The ovaries were brought from the slaughterhouse in Riyadh, Saudi Arabia, in a 0.9% NaCl saline solution and brought to the laboratory within 2-3 hours at a temperature of 25-35°C. Only the collected oocytes with two layers or more of cumulus cells (CCs) and uniform ooplasm were used. Five repetitions were made in each season of the year, followed by their ripening in the laboratory and a follow-up ripening after 24 hours. Observe the maturity rate after the stain and the expansion of cumulus cells and compare them with other seasons. Result: The mean maturation rates of MII and MI oocytes were not significantly different between all seasons. Additionally, there were no significant changes in the cumulus-oocyte complex before maturation in all seasons. However, after maturation, a highly significant difference (P less than 0.001) was found between the spring season before and after maturation (156.31±17.68 mm) and the rest of the seasons (summer; 169.89±19.96 mm; autumn; 176.66±20.14 mm; and winter; 188.84±24.50 mm). In order to improve in vitro maturation for following investigations, such as in vitro fertilization or cloning and in vitro embryo development, the ideal season should be chosen for collecting oocytes.

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Section: Resultsmentioning

confidence: 99%

Seasonal Influence on Oocyte Recovery Rate, Quality and in vitro Maturation in Cows

Ammari¹,

Alhimaidi²,

Amran³

et al. 2023

IJAR

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show abstract

“…Therefore, different approaches to the optimisation of the IVEP stages have been employed. The first strategy of the in vitro maturation was improved through stimulation by luteinising hormones, to stimulate their meiotic progression to metaphase II (MII) (de Oliveira Santos et al 2018;de Oliveira et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Kaabi¹,

Barakat²,

Alajmi³

et al. 2022

Vet. Med. - Czech

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The present study was undertaken to investigate the possible stimulating effects of Nigella sativa (N. sativa) honey, natural Saudi Sider honey, and honeybee pollen to the in vitro maturation (IVM) medium of sheep oocytes on their subsequent development. Hence, immature oocytes were exposed to various concentrations of natural Nigella sativa (N. sativa), and Saudi Sider honey (5, 10, and 20%), as well as honeybee pollen (1, 10, 50 μg/ml) during an in vitro maturation period (24 hours). After the exposure time, the maturation rate, glutathione (GSH) concentration, and candidate gene expression (GDF-9, MPF, CMOS, IGF-1, and BAX) were evaluated. Our results showed that the maturation rate was higher in the groups challenged with the lowest level of the bee products (5% and 1 μg/ml) when compared with that in the control group; where the mean number of oocytes in the metaphase II stage reached 0.360 for the honeybee pollen-treated group, 0.293 for the N. sativa-treated group, and 0.203 for the natural Saudi Sider honey-treated group. The glutathione level was significantly increased in the group exposed to N. sativa honey when compared with the other groups. Concerning the gene expression results, the Saudi Sider honey treatment showed the best results for all the genes except the CMOS gene, which was significantly higher than the GI and GII groups and lower than the GIV group and the BAX gene which did not show a significant difference when compared with the other groups. In conclusion, the addition of natural honey and honeybee pollen at a low concentration to an IVM medium improved the in vitro maturation rate, increased the glutathione level, and gene expression of the in vitro matured ovine oocytes.

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“…However, oocyte competence does not depend only on intrinsic factors, but is also related to the type of protocol used in the laboratory, the semen, and method of obtaining oocytes Research, Society and Development, v. 10, n. 2, e16110212264, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i2.12264 (Merton et al, 2003). Several authors have emphasized the improvement of oocyte quality in the supplementation of oocytes with antioxidant substances, to reduce oxidative stress (Oliveira et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Factors affecting the in vitro production of bovine embryos in a commercial program

Nogueira

Souza

Puelker

et al. 2021

RSD

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Variability in the production of bovine blastocysts per session of ultrasound-guided follicularaspiration(OPU) is still an obstacle in commercial production.Thus, the objective of the current work was to verify factors that influence in vitro embryo production (PIVE), using data from a commercial laboratory.Data from 2014 to 2016 were analyzed, referring to 799 OPU sessions of adult bovine females of the breeds: Nelore (n=83), Girolando (n=73), Brangus (n=49), HPB (n=20), and Senepol (n=10).The influence of five variables was analyzed: breed, genetic group, frequency of aspiration, type of semen (sexed/conventional), and seasonality (spring/summer vs. autumn/winter) on the rate of viable oocytes, cleavage, and blastocyst production (bl).The protocols for the in vitroproduction of embryos (IVP) followed the routine established by the partner laboratory of the present study. The statistical analysis of the data was performed using SAS, with the Chi-square test.The Girolando and Nelore breeds had a higher (p=0.0001) number of blastocysts/OPU session, as well as the Bos indicus blood group.Blastocyst production was higher (p=0.0059) with sexed semen compared to conventional semen (6.6 x 5.2 bl/OPU session); a higher frequency of aspirations also increased (p=0.005) the number of bl/OPU session (7.7 x 5.9).We conclude that the in vitro production of bovine embryos is influenced by the analyzed factors and knowledge of these variables could guide the commercial use of OPU-PIVE.

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Bioguided isolation of compounds with antioxidant activity to improve the in vitro maturation of mammalian oocytes

Cited by 4 publications

References 45 publications

Seasonal Influence on Oocyte Recovery Rate, Quality and in vitro Maturation in Cows

Seasonal Influence on Oocyte Recovery Rate, Quality and in vitro Maturation in Cows

Ameliorative effect of bee products on in vitro maturation of sheep oocytes

Factors affecting the in vitro production of bovine embryos in a commercial program

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