Biohydrogen Machinery: Recent Insights, Genetic Fabrication, and Future Prospects

Madavi, Tanushree Baldeo; Chauhan, S. M. S.; Jha, Meenakshi; Choi, Kwon‐Young; Pamidimarri, Sudheer D. V. N.

doi:10.1002/ceat.202000527

Cited by 2 publications

(2 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrogen is also produced through physio‐chemical options, which are energy‐intensive, cost‐incurred processes and not eco‐friendly 96–98 . Enzymes like hydrogenases and nitrogenases are naturally engineered to catalyze the synthesis of hydrogen using various feedstocks 99 . Whole‐cell biocatalysts like E. coli , Clostridia , cyanobacteria and microalgae are preferred for hydrogen production 96 .…”

Section: Whole‐cell Biocatalysis: Changing Paradigm Of Biorefineriesmentioning

confidence: 99%

“…[96][97][98] Enzymes like hydrogenases and nitrogenases are naturally engineered to catalyze the synthesis of hydrogen using various feedstocks. 99 Whole-cell biocatalysts like E. coli, Clostridia, cyanobacteria and microalgae are preferred for hydrogen production. 96 Photoautotrophic organisms like cyanobacteria and microalgae harness native machinery to harvest atmospheric CO 2 and solar energy to generate biohydrogen, which is considered to be a good option.…”

Section: Hydrogen: Toward Carbon-less Fuelmentioning

confidence: 99%

See 1 more Smart Citation

Whole‐cell biocatalysis: Advancements toward the biosynthesis of fuels

Madavi

Chauhan

Keshri

et al. 2021

Biofuels Bioprod Bioref

Self Cite

View full text Add to dashboard Cite

The availability of robust microbial systems has facilitated the implementation of greener technology replacing existing less sustainable chemical technologies. Whole-cell biocatalysis has empowered the biological production of chemicals and biofuels, replacing labor-intensive traditional chemical catalysis. Whole-cell biocatalysis offers new avenues to use sustainable raw/waste biomass as a substrate for biotransformation into industrially important compounds. Using a native or nonnative microbial cell system as a chassis for developing a suitable cell catalyst requires multiple-level adjustments owing to the target product. Enzymes, the critical entity of biocatalysis, are an important factor influencing biocatalysis efficiency; whole cells provide optimal conditions to the enzymes or enzyme cascades for maximum productivity. Advancements in system biology and metabolic engineering techniques have led to the rational design of whole-cell catalysts for the suitable production of green fuels. Traditional enzyme catalysts are limited by issues such as enzyme stability and repeatability, laborious downstream processing and enzyme production technicalities, but whole-cell biocatalysis could bypass those bottlenecks. Thus, the application of whole cells in the catalysis and production of fuels has progressed greatly in the past couple of decades. This review focuses on detailing the concept of whole-cell biocatalysis and its advances in the production of biofuels such as alcohols and fatty acidbased, terpenoid-derived and carbon-free fuels. The technical advancements in various hosts such as Escherichia coli, Saccharomyces cerevisiae, Corynebacterium glutamicum and Synechococcus elongatus to establish whole-cell biocatalysis are summarized. In addition, system engineering toward the optimum production of various biofuels is added to the discussion.

show abstract

Section: Whole‐cell Biocatalysis: Changing Paradigm Of Biorefineriesmentioning

confidence: 99%