Biohydrogen Production from Hydrolysates of Selected Tropical Biomass Wastes with Clostridium Butyricum

Abstract

Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0.05 L-cysteine, 0.05 MgSO4·7H2O, 0.1 MnSO4·H2O and 0.3 FeSO4·7H2O, which was very different from that for cell growth. Sugarcane bagasse and Jatropha hulls were selected as typical tropical biomass wastes to produce sugars via a two-step acid hydrolysis for hydrogen production. Under the optimized fermentation conditions, H2 yield (mol H2/mol-total reducing sugar) was 2.15 for glucose, 2.06 for bagasse hydrolysate and 1.95 for Jatropha hull hydrolysate in a 3L fermenter for 24 h at 35 °C, with H2 purity of 49.7–64.34%. The results provide useful information and basic data for practical use of tropical plant wastes to produce hydrogen.

Integrated System Technology of POME Treatment for Biohydrogen and Biomethane Production in Malaysia

“…Therefore, for a future prospect, a large-scale integrated system should be considered, especially from the agricultural and food and beverages industries in Malaysia. While reducing CH 4 emissions from an open-ponding system used, a clean H 2 and CH 4 could also be simultaneously generated using a biological treatment in an integrated bioreactor.…”

“…It is considered the best and most effective fuels for transportation. This is because, when H 2 is combusted (only water vapor is produced with the absence of carbon monoxide (CO), the energy yields are 2.75 times higher (122 kJ/g) than hydrocarbon fuels [3,4]. This can minimize environmental problems and makes H 2 a future fuel, which has drawn significant attention to the world.…”

Optimization of biohydrogen production using acid pretreated corn stover hydrolysate followed by nickel nanoparticle addition

“…And its by‐product (corn stover; CS) is produced in the large quantities annually . Albeit, many different pretreatment approaches such as acid/base cellulosic saccharification, steam explosion, radioactive degradation have been proposed and investigated, the dilute acid hydrolysis is still found to be attractive in the aspects of easiness and cost‐effectiveness in operation . During DF, various hydrogen formations related metabolic pathways undertook either sequentially or simultaneously.…”

“…18,19 Albeit, many different pretreatment approaches such as acid/base cellulosic saccharification, steam explosion, radioactive degradation have been proposed and investigated, the dilute acid hydrolysis is still found to be attractive in the aspects of easiness and cost-effectiveness in operation. 18,20,21 During DF, various hydrogen formations related metabolic pathways undertook either sequentially or simultaneously. Taking the intermediate pyruvate for example, it is metabolized into the liquid metabolites such as acetic acid, propionic acid, butyric acid, valeric acid, and ethanol.…”

Integrating uniform design and response surface methodology to optimize thiacloprid suspension

“…It is especially appropriate for modelling and analysing multi-factor experiments to clarify how the single and interaction effects influence the responses based on a series of statistical techniques1213. Currently, RSM has been widely applied in food chemistry, pharmaceuticals, material science, chemical engineering and other fields14. We have also demonstrated its favourable adaptability in pesticide formulations1516.…”