Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood

Das, Anupam A. K.; Medlock, Jevan; He, Liang; Nees, Dieter; Allsup, David; Madden, Leigh A.; Paunov, Vesselin N.

doi:10.1039/c9tb00679f

Cited by 8 publications

(29 citation statements)

References 31 publications

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“…To prevent both types of cells from coming too close to the imprint and becoming trapped by the van der Waals attraction, we treated the bPEI coated imprint with an additional layer of Pluronic surfactant (Poloxamer 407) which minimizes nonspecific attachment of the cells to the imprint. [28,29] The effect of the Poloxamer 407 treatment is not related to changing of the wettability of the bioimprint surface rather than offsetting the cells from the surface which prevents non-specific adhesion. [28,29] The idea behind this combination of treatments is that when the approaching cells match closely the imprint cavities, the weak electrostatic attraction is amplified by the large area of contact with the imprint cavity.…”

Section: Bioimprint Fabricationmentioning

confidence: 99%

“…[28,29] The effect of the Poloxamer 407 treatment is not related to changing of the wettability of the bioimprint surface rather than offsetting the cells from the surface which prevents non-specific adhesion. [28,29] The idea behind this combination of treatments is that when the approaching cells match closely the imprint cavities, the weak electrostatic attraction is amplified by the large area of contact with the imprint cavity. For PBMCs, which do not closely fit in the cavities and make only a point contact, the subsequent flushing step allows their removal from the imprint while the CTCs remain on the imprint and concentrate on its surface, which facilitates their detection.…”

Section: Bioimprint Fabricationmentioning

confidence: 99%

“…[4] This high mortality is mainly because of the propensity of the and size. [25,28,29,50] When a matching cell comes in contact with the bioimprint, it becomes strongly attracted to its surface, as the bioimprint-cell attraction is amplified by the increased contact area which results in cell shape and size recognition. [30] Dickert et al [31] pioneered a soft lithographic technique by imprinting whole yeast cells on the sensor surface of a quartz crystal microbalance (QCM) which allowed selective capture of different types of yeast.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, bioimprinting technology was developed to produce colloid antibodies, which use cell shape recognition to selectively target microbial cells of specific shape. [26,27] Furthermore, Das et al [28] successfully developed bioimprinting technology to separate leukemic myeloblasts from healthy peripheral blood cells. A promising approach for targeting specific blood cancer cells and their separation from peripheral blood based on generic bioimprints has also been recently reported.…”

Section: Introductionmentioning

confidence: 99%

“…A promising approach for targeting specific blood cancer cells and their separation from peripheral blood based on generic bioimprints has also been recently reported. [25,28,29] Personalized bioimprints that may have the potential to target specific cell types and do not require major morphological differences from healthy cells or express particular surface moieties.…”

Section: Introductionmentioning

confidence: 99%

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Bioimprint Mediated Label‐Free Isolation of Pancreatic Tumor Cells from a Healthy Peripheral Blood Cell Population

et al. 2020

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tumor to early metastasize and spread [5] and even the smallest primary lesions often show perineural and lympho-vascular invasion. [6] More recently, the 5-year survival rates have improved slightly but differs from country to country, based on management approaches such as early surgical removal, stage, absence of lymph node metastasis. Worldwide, survival is generally around 5% [2] and it is the fifth most common cause of cancer death in the UK [7] and Europe. [8] In the U.S, PC is the fourth leading cause of cancer death [9] and second most common gastrointestinal malignancy. Surgical resection of the tumor is associated with the best patient outcomes but as stated above due to early tumor spread this is not an option in many cases. Pancreatic cancer is a malignancy that is very difficult to detect early, it is rarely cured (less than 1%) and even small tumors have often metastasized early to the liver. [10] This is preceded by release of circulating tumor cells (CTCs) which spread to the patients organs through the peripheral blood circulatory system. [11,49] Pancreatic CTCs can be used as a biomarker for potential earlier detection of cancer [12] however, there is no proven technology which could capture and detect these cells. [12-14] CTCs usually have oval shapes within the blood and there are size and shape differences between the pancreatic tumor cells and normal blood cells, which allows the latter to be selectively targeted by cell shape recognition. These features may potentially allow pancreatic CTCs to be extracted from blood samples by cell shape and size recognition using bioimprints produced for these specific types of cells. Available methods for the collection or detection of CTCs take advantage of various physical properties of the target cells and their surfaces. [15-24] Bioimprints have recently been recognized for their great potential for biomedical diagnostics and targeted therapies as they can effectively capture cells of specific shape and morphology, isolating them or delivering biocides directly to their surface. [25-27] Bioimprints are exact physical copies of the surface of a cell layer which can be produced by impressing the cells with a layer of curable polymer or other material. [25,27-29] The bioimprinting process produces a polymeric cast of the whole cell layer, producing a negative replica of the cells shape New techniques are required for earlier diagnosis and response to treatment of pancreatic cancer. Here, a label-free approach is reported in which circulating pancreatic tumor cells are isolated from healthy peripheral blood cells via cell bioimprinting technology. The method involves pre-fabrication of pancreatic cell layers and sequential casting of cell surfaces with a series of custom-made resins to produce negative cell imprints. The imprint is functionalized with a combination of polymers to engineer weak attraction to the cells which is further amplified by the increased area of contact with the matching cells. A flow-through bioimprint chip is designed and tested...

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Section: Bioimprint Fabricationmentioning

confidence: 99%

Section: Bioimprint Fabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioimprint Mediated Label‐Free Isolation of Pancreatic Tumor Cells from a Healthy Peripheral Blood Cell Population

et al. 2020

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Deposition Methods for the Integration of Molecularly Imprinted Polymers (MIPs) in Sensor Applications

Caldara

Wissen

Cleij

et al. 2023

Advanced Sensor Research

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Offering high specificity and selectivity, molecularly imprinted polymers (MIPs) are synthetic polymeric affinity reagents that have become increasingly popular over the last couple of decades. Due to their long-term chemical and physical stability and low production cost, they have become an increasingly popular choice of receptor in the realm of sense. MIPs have therefore been associated with the detection of small molecules, proteins, cells, and pathogens, proving a highly robust and useful tool in the production of next-gen sensing platforms. This said, the development of these sensors pivots on one simple fact; these receptors have to be deposited onto a substrate for their desired application. The deposition of MIPs during sensor fabrication is therefore of great importance, with the field utilizing an array of mechanical and chemical deposition methods to achieve this. To this end, this review, therefore, sets aim at coalescing these different deposition approaches, classifying them, and outlining their utility when it comes to receptor design and integration. Thus, offering a knowledge base on current deposition methods, potential future approaches and analyzing where the MIP deposition field is tending toward.

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Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

2020

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We have developed and tested a novel ELISA-like approach for bacterial detection based upon selective adhesion of targeted bacteria to microwells with prefabricated bacterial bioimprints. Bioimprints were produced from three bacterial species; Escherichia coli (Gram-negative), Rhodococcus rhodochrous (Gram-positive) and Sarcina aurantiaca (Gram-negative), by using molding with curable silicone from dense layers of bacterial cells deposited on a glass substrate. We demonstrated that the surface functionalized whole cell bioimprints were able to selectively recognize and bind their own bacterial cell type. In order to detect target bacteria that are bound to the matching bioimprint, we also developed silica nanoparticles dual-functionalized with (3-glycidyloxypropyl)trimethoxysilane (GLYMO) coupled with 4-hydroxyphenylboronic acid (4-HPBA), SiO 2 NPs/GLYMO/4-HPBA, which were further conjugated with horseradish peroxidase (HRP). Bacterial detection was demonstrated to work in the established ELISA-like protocol using the colorimetric reaction of the conjugated HRP with 3,3′,5,5′tetramethylbenzidine (TMB). The bioimprints were used instead of capture antibodies and HRP-coated dual functionalized silica nanoparticles instead of a secondary antibody with TMB as the enzyme-converted reagent, producing a colored byproduct. This bacterial bioimprint-based detection method does not rely on any antibodies, uses stable and inexpensive reagents, and could potentially find application for rapid diagnostics of bacterial pathogens in clinical samples at the point of care. K E Y W O R D S bacteria, bioimprints, ELISA, functionalized silica nanoparticles, horse radish peroxidase 1 INTRODUCTION Bacterial infections remain as a serious issue in modern health care. [1-3] In recent years, this need for the rapid diagnosis of bacterial infections has resulted in the This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood

Abstract: We report a large scale preparation of bioimprints of layers of cultured leukemic HL60 cells which can perform cell shape and size recognition from a mixture with peripheral blood mononuclear cells (PBMCs).

Cited by 8 publications

References 31 publications

Bioimprint Mediated Label‐Free Isolation of Pancreatic Tumor Cells from a Healthy Peripheral Blood Cell Population

Bioimprint Mediated Label‐Free Isolation of Pancreatic Tumor Cells from a Healthy Peripheral Blood Cell Population

Deposition Methods for the Integration of Molecularly Imprinted Polymers (MIPs) in Sensor Applications

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

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