Bioinformatic analysis of microRNA expression in Parkinson’s disease

Abstract

Abstract. Parkinson's disease (PD) is a type of movement disorder caused by loss of dopamine-producing neurons in the midbrain. In order to identify the synergistic microRNA (miRNA) pattern in PD, miRNA and mRNA double expression profiles of PD were downloaded. Differentially expressed miRNA and mRNA were identified [P<0.01, following false discovery rate (FDR) correction]. A cumulative hypergeometric distribution test was then performed to identify synergistic miRNAs (P<0.01, following FDR correction). Gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations were performed to analyze the miRNA regulatory target genes. Subsequently, a synergistic miRNA network was constructed and miRNAs exhibiting a high degree were identified. In total, 200 differentially expressed miRNA and 2,966 differentially expressed mRNA were identified. In addition, 1,502 synergistic miRNA interactions were identified, and miRNAs regulated 304 target genes in total. The GO and KEGG analysis demonstrated that these target genes were enriched in biosynthetic and cellular biosynthetic processes, the assembly of cellular components in morphogenesis, mitogen-activated protein kinase signaling, myometrial relaxation and contraction pathways as well as calcium regulation. The miRNA network demonstrated that miR-627, miR-634, miR-514, miR-563 and miR-613 had a high degree. miRNA with a high degree may be associated with the pathogenesis of PD and, therefore, may assist in the diagnosis and therapy of PD.

Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease

“…Recent work has emphasised the association of selected microRNAs to PD [27,122,123] and roles for microRNAs in neuroinflammation have received increased attention [25,124]. As neuroinflammation is thought to be a facilitator of PD pathogenesis, and was observed in the pre-motor PD model used in this study [32,125], we assessed changes in relative expression levels of three inflammatory and hypoxia associated microRNAs: miR21, miR155, and miR210.…”

MicroRNA-181a Regulates Apoptosis and Autophagy Process in Parkinson’s Disease by Inhibiting p38 Mitogen-Activated Protein Kinase (MAPK)/c-Jun N-Terminal Kinases (JNK) Signaling Pathways

“…Several miRNAs have been identified to be abnormally expressed in the brain tissues of patients or animals with PD and are responsible for the development of DA neurons [12,26–28]. Thus, many miRNAs are regarded as novel biomarkers for molecular diagnosis and potential therapy targets.…”

Overexpression of miR‑185 inhibits autophagy and apoptosis of dopaminergic neurons by regulating the AMPK/mTOR signaling pathway in Parkinson's disease

“…MicroRNAs (miRNAs) are small, endogenous, noncoding RNA molecules, which have exhibited potential as biomarkers or therapeutic targets for PD ( 11 – 13 ). Bioinformatics analysis has revealed that ~200 miRNAs, including miR-627, miR-634, miR-514, miR-563 and miR-613, are differentially expressed between PD and healthy tissue samples ( 14 ), suggesting that miRNAs may participate in PD pathogenesis and progression. In addition, upregulation of miR-124 has been demonstrated to regulate autophagy and apoptotic processes, and thus reduce the loss of DAergic neurons in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model of PD ( 15 ).…”