Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

Zhao, Ming; Xu, Longbiao; Qian, Hui

doi:10.1097/md.0000000000021186

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…23 , 24 Analysis of the expression of VEGF, Bcl-2, and MT1-MMP in this research revealed that miR-423-5p was a good predictor of HFmrEF, which could accurately indicate an improved cardiac function in those patients following cardiac rehabilitation treatment. Combined with previous studies, our data suggest that miR-423-5p may induce cardiomyocyte apoptosis by regulating the PI3k/AKT pathway 25 and inhibiting O-type N -acetylglucosaminyltransferase (OGT). 26 Meanwhile, cardiac rehabilitation may improve cardiac function, promote physiological structural changes in the heart, and inhibit cardiac physiological remodeling 27 by regulating endothelial progenitor cells via miR-423-5p.…”

Section: Discussionsupporting

confidence: 77%

Functional Significance of Cardiac Rehabilitation-Regulated Expression of Circulating MicroRNA-423-5p in Hypertensive Patients with Heart Failure with a Moderately Reduced Ejection Fraction

Huang¹,

Zhang²,

Nong³

et al. 2022

Anatolian J Cardiol

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Background Hypertension is a vital risk factor for heart failure, while cardiac rehabilitation can effectively improve cardiac function of heart failure patients. This study aimed to determine the impact of cardiac rehabilitation on microRNA-423-5p in hypertensive patients with heart failure with a moderately reduced ejection fraction. Methods Sixty hypertensive patients with heart failure with a moderately reduced ejection fraction were randomly divided into cardiac rehabilitation group and positive control group with 30 cases per group, while 30 hypertensive patients without heart failure were recruited as negative control group. The cardiac rehabilitation group and positive control group were treated with 1-month cardiac rehabilitation combined with the routine treatment and routine treatment only, respectively. The New York Heart Association classification, 6-minute walking test, and color Doppler echocardiography were adopted to detect cardiac function. Meanwhile, the expression of microRNA-423-5p and N-terminal pro-B-type natriuretic peptide was determined via Real-Time Fluorescence Quantitative PCR and electrochemiluminescence immunoassay. The diagnostic potential of microR-423-5p and N-terminal pro-B-type natriuretic peptide was assessed by ROC curve analysis and multivariate linear regression model. Results Patients in cardiac rehabilitation group displayed significantly lower expression of microR-423-5p and better results of New York Heart Association classification, 6-minute walking test, and color Doppler echocardiography than those in positive control group ( P < .05). ROC analysis showed that microR-423-5p (AUC = 0.785; 95% CI: 0.686-0.865; sensitivity = 73.33%; specificity = 73.33%) had better specificity and accuracy than N-terminal pro-B-type natriuretic peptide (AUC = 0.721; 95% CI: 0.617-0.811; sensitivity = 81.67%; specificity = 63.33%). Conclusion MicroR-423-5p was implicated in left ventricular hypertrophy and might be a potential biomarker for assessing the therapeutic effect of cardiac rehabilitation on hypertensive patients with heart failure with a moderately reduced ejection fraction.

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Section: Discussionsupporting

confidence: 77%

Functional Significance of Cardiac Rehabilitation-Regulated Expression of Circulating MicroRNA-423-5p in Hypertensive Patients with Heart Failure with a Moderately Reduced Ejection Fraction

Huang¹,

Zhang²,

Nong³

et al. 2022

Anatolian J Cardiol

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“…A primary limitation in these studies remains the small sample sizes within the GEO datasets explored. However, it is noteworthy that some studies have validated their findings in distinct GEO datasets [ 101 , 102 , 103 ].…”

Section: Resultsmentioning

confidence: 97%

The Role of Epigenetics in Brain Aneurysm and Subarachnoid Hemorrhage: A Comprehensive Review

Fernández-Pérez,

Macias-Gómez,

Suárez-Pérez

et al. 2024

IJMS

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This comprehensive review explores the emerging field of epigenetics in intracranial aneurysm (IA) and aneurysmal subarachnoid hemorrhage (aSAH). Despite recent advancements, the high mortality of aSAH needs an understanding of its underlying pathophysiology, where epigenetics plays a crucial role. This review synthesizes the current knowledge, focusing on three primary epigenetic mechanisms: DNA methylation, non-coding RNA (ncRNA), and histone modification in IA and aSAH. While DNA methylation studies are relatively limited, they suggest a significant role in the pathogenesis and prognosis of IA and aSAH, highlighting differentially methylated positions in genes presumably involved in these pathologies. However, methodological limitations, including small sample sizes and a lack of diverse population studies, temper these results. The role of ncRNAs, particularly miRNAs, has been more extensively studied, but there are still few studies focused on histone modifications. Despite methodological challenges and inconsistent findings, these studies underscore the involvement of miRNAs in key pathophysiological processes, including vascular smooth muscle regulation and the inflammatory response. This review emphasizes methodological challenges in epigenetic research, advocating for large-scale epigenome-wide association studies integrating genetic and environmental factors, along with longitudinal studies. Such research could unravel the complex mechanisms behind IA and aSAH, guiding the development of targeted therapeutic approaches.

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“…Despite the small study size, this independent analysis can be used to confirm that miR-424-5p is a biomarker for the beginning of CVD. Others also reported miR-424-5p to be a biological marker of intracranial aneurism [ 50 ] or predictor of risk of fatal myocardial infarction whilst combined with Framingham score [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Upregulated anti-angiogenic miR-424-5p in type 1 diabetes (model of subclinical cardiovascular disease) correlates with endothelial progenitor cells, CXCR1/2 and other parameters of vascular health

et al. 2021

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Background In spite of clinical progress, cardiovascular disease (CVD) remains the predominant cause of mortality worldwide. Overexpression studies in animals have proven miR-424-5p to have anti-angiogenic properties. As type 1 diabetes mellitus (T1DM) without CVD displays endothelial dysfunction and reduced circulating endothelial progenitor cells (cEPCs), it offers a model of subclinical CVD. Therefore, we explored miR-424-5p, cytokines and vascular health in T1DM. Methods Twenty-nine well-controlled T1DM patients with no CVD and 20-matched controls were studied. Cytokines IL8, TNF-α, IL7, VEGF-C, cEPCs/CD45dimCD34+CD133+ cells and ex-vivo proangiogenic cells (PACs)/fibronectin adhesion assay (FAA) were measured. MiR-424-5p in plasma and peripheral blood mononuclear cells (PBMC) along with mRNAs in PBMC was evaluated. Results We found an elevation of IL7 (p = 0.008), IL8 (p = 0.003), TNF-α (p = 0.041), VEGF-C (p = 0.013), upregulation of mRNA CXCR1 (p = 0.009), CXCR2 (p < 0.001) and reduction of cEPCs (p < 0.001), PACs (p < 0.001) and FAA (p = 0.017) in T1DM. MiR-424-5p was upregulated in T1DM in PBMC (p < 0.001). MiR-424-5p was negatively correlated with cEPCs (p = 0.006), PACs (p = 0.005) and FAA (p < 0.001) and positively with HbA1c (p < 0.001), IL7 (p = 0.008), IL8 (p = 0.017), VEGF-C (p = 0.007), CXCR1 (p = 0.02) and CXCR2 (p = 0.001). ROC curve analyses showed (1) miR-424-5p to be a biomarker for T1DM (p < 0.001) and (2) significant upregulation of miR-424-5p, defining subclinical CVD, occurred at HbA1c of 46.5 mmol/mol (p = 0.002). Conclusion We validated animal research on anti-angiogenic properties of miR-424-5p in T1DM. MiR-424-5p may be a biomarker for onset of subclinical CVD at HbA1c of 46.5 mmol/mol (pre-diabetes). Thus, miR-424-5p has potential use for CVD monitoring whilst anti-miR-424-5p-based therapies may be used to reduce CVD morbidity/mortality in T1DM.

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Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

Cited by 12 publications

References 35 publications

Functional Significance of Cardiac Rehabilitation-Regulated Expression of Circulating MicroRNA-423-5p in Hypertensive Patients with Heart Failure with a Moderately Reduced Ejection Fraction

Functional Significance of Cardiac Rehabilitation-Regulated Expression of Circulating MicroRNA-423-5p in Hypertensive Patients with Heart Failure with a Moderately Reduced Ejection Fraction

The Role of Epigenetics in Brain Aneurysm and Subarachnoid Hemorrhage: A Comprehensive Review

Upregulated anti-angiogenic miR-424-5p in type 1 diabetes (model of subclinical cardiovascular disease) correlates with endothelial progenitor cells, CXCR1/2 and other parameters of vascular health

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