Bioinspired Collagen-Apatite Nanocomposites for Bone Regeneration

Liu, Shuai; Sun, Yue; Fu, Yu; Chang, Datong; Fu, Cuicui; Wang, Gaonan; Liu, Yan; Tay, Franklin R.; Zhou, Yan-Heng

doi:10.1016/j.joen.2016.04.027

Cited by 26 publications

(18 citation statements)

References 33 publications

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“…In this study, we first reported the use of biomimetic IMC in combination with autologous PDLSCs for the regeneration of large bone defects in minipigs. Compared with rat critical-sized mandibular defects of approximately 5 mm in diameter in our previous studies 27 , 28 , the defect area of approximately 2 cm width × 3 cm length × 0.5 cm depth) in this study is an order of magnitude greater. The IMC still possesses an excellent bone regeneration and vascularization potential.…”

Section: Discussioncontrasting

confidence: 55%

“…The 3-D macroporous IMC scaffold was prepared using a modified bottom-up biomimetic approach 27 , 28 . Briefly, type I tropocollagen solution (Corning, 10 mg/mL, pH < 2) was slowly injected into a dialysis flask (3500 Da), which was immersed in a mineralization solution (pH = 7.4) containing poly(acrylic acid) (0.25 mM, Mw 2000, Sigma-Aldrich), type I white Portland cement (0.2 g/mL, Lehigh Cement Co.) and simulated body fluid.…”

Section: Methodsmentioning

confidence: 99%

“…Biomimetic bone-like intrafibrillarly-mineralized collagen (IMC) was successfully fabricated in our previous studies and was proven to be a biocompatible and osteoconductive material with a proper degradation rate 23 – 28 . An IMC combined with BMSCs could promote bone regeneration among rats with critical-sized mandibular bone defects 27 , 28 . However, large animal studies with high clinical relevance are required to evaluate the bone regeneration potential of IMC 29 , 30 .…”

Section: Introductionmentioning

confidence: 99%

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Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells

Zhang

Yan

Cui

et al. 2017

Sci Rep

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Biomimetic intrafibrillarly-mineralized collagen (IMC) is a promising scaffold for bone regeneration because of its structural and functional similarity to natural bone. The objective of this study was to evaluate the bone regeneration potential of IMC loaded with autologous periodontal ligament stem cells (PDLSCs) in large bone defects in minipigs. A macroporous IMC with a bone-like subfibrillar nanostructure was fabricated using a biomimetic bottom-up approach. Non-healing full thickness defects were established on the cranial bone in minipigs, and IMC and hydroxyapatite (HA) scaffolds seeded with autologous PDLSCs were implanted into these defects. Computed tomographic imaging, histology staining, and atomic force microscopy were applied to evaluate to the quantity, micro/nano structures, and mechanical performance of the neo-bone after 12 weeks of implantation. Compared with HA, IMC showed superior regeneration properties characterized by the profuse deposition of new bony structures with a normal architecture and vascularization. Immunohistochemistry showed that the runt-related transcription factor 2 and transcription factor Osterix were highly expressed in the neo-bone formed by IMC. Furthermore, the nanostructure and nanomechanics of the neo-bone formed by IMC were similar to that of natural bone. This study provides strong evidence for the future clinical applications of the IMC-based bone grafts.

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Section: Discussioncontrasting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells

Zhang

Yan

Cui

et al. 2017

Sci Rep

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“…The Col-Ap nanocomposite scaffold was able to activate boneforming cells, promote inward vascularization, as well as induce the synthesis of the ECM mediated by increased TGFb1. (Liu et al, 2016). In addition, Haj et al demonstrated that nanofibrous HA/chitosan (nHAp/CTS) scaffolds seeded with MSCs were superior to membranous HAp/CTS in a rat FIGURE 1 | Schematic preparation of ECM-based scaffold in bone regeneration.…”

Section: Ecm-modified Biomaterials Scaffoldmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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Bone regeneration repairs bone tissue lost due to trauma, fractures, and tumors, or absent due to congenital disorders. The extracellular matrix (ECM) is an intricate dynamic bio-environment with precisely regulated mechanical and biochemical properties. In bone, ECMs are involved in regulating cell adhesion, proliferation, and responses to growth factors, differentiation, and ultimately, the functional characteristics of the mature bone. Bone ECM can induce the production of new bone by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and the absorption of bone by osteoclasts. With the rapid development of bone regenerative medicine, the osteoinductive, osteoconductive, and osteogenic potential of ECM-based scaffolds has attracted increasing attention. ECM-based scaffolds for bone tissue engineering can be divided into two types, that is, ECM-modified biomaterial scaffold and decellularized ECM scaffold. Tissue engineering strategies that utilize the functional ECM are superior at guiding the formation of specific tissues at the implantation site. In this review, we provide an overview of the function of various types of bone ECMs in bone tissue and their regulation roles in the behaviors of osteoblast-lineage cells and osteoclasts. We also summarize the application of bone ECM in bone repair and regeneration. A better understanding of the role of bone ECM in guiding cellular behavior and tissue function is essential for its future applications in bone repair and regenerative medicine.

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“…The use of antimicrobial agents for the chemomechanical preparation of infected root canals, followed by obturation and coronal repair, provides beneficial results. However, sometimes infective microorganisms that reside in the root canals may gradually gain multiple resistance to drugs or disinfection methods, and this would eventually lead to the failure of root canal treatment [ 1 , 2 ] and more severe problems, such as chronic apical periodontitis and local bone resorption [ 3 ]. In such cases, endodontic surgery is commonly required to eradicate these microorganisms, and such surgical procedures usually include the mechanical removal of part of the infected tooth, followed by irrigation of antibacterial agents into the cavity before manual filling of cavities with impermeable body fluids, which have the ability to adhere to dentinal walls [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Mineral Trioxide Aggregate Mixed with 5-Aminolevulinic Acid for the Photodynamic Antimicrobial Strategy in Hard Tissue Regeneration

Huang

et al. 2018

Materials

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Aminolevulinic acid (ALA) based photodynamic antimicrobial strategy can provide good antimicrobial effects and be used for medical applications. The aim of this study was to apply this strategy to Mineral Trioxide Aggregate (MTA), which is commonly used as a filling material for root endings and by doing so, to increase the bactericidal capability of MTA, as well as to investigate its characterization, cytocompatibility, and odontogenic differentiation potential. MTA is known to be a derivative of calcium silicate (CS). In this study, MTA specimens with or without ALA and light treatment were prepared. Diametral tensile strength values (DTS), setting durations, X-ray diffraction (XRD) spectra, apatite-mineralization, and antimicrobial abilities of the MTA, were also analyzed. Human dental pulp cells (hDPCs) can proliferate into the newly formed matrix and differentiate into odontoblasts to reinforce and strengthen the root. Levels of hDPCs proliferation and its odontogenic capabilities when cultured on MTA with ALA and light treatment, and the percentages of cells existing in the various cell cycle stages, were further evaluated in this study. The results indicated that MTA added ALA with light treatment had greater antibacterial ability and cytocompatibility, compared to MTA alone. A higher percentage S phase of the cells cultured on MTA added ALA with light treatment was observed. Furthermore, hDPCs cultured on MTA added ALA with light treatment had the highest expression levels of the odontoblastic differentiation markers. ALA has great antimicrobial efficiency and is a potential material for future medical applications. ALA-based photodynamic antibacterial strategy applied in the MTA has great antibacterial ability, cytocompatibility, and odontoblastic differentiation potential, and can facilitate the development of root canal treatment.

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Bioinspired Collagen-Apatite Nanocomposites for Bone Regeneration

Abstract: The present study lays the foundation for the development of Col-Ap nanocomposite-based bone grafts for future clinical applications in bone regeneration of large periapical lesions after apical curettage or apicoectomy.

Cited by 26 publications

References 33 publications

Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells

Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells

The Bone Extracellular Matrix in Bone Formation and Regeneration

Mineral Trioxide Aggregate Mixed with 5-Aminolevulinic Acid for the Photodynamic Antimicrobial Strategy in Hard Tissue Regeneration

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