Bioinspired enzymatic synthesis of silica nanocrystals provided by recombinant silicatein from the marine sponge Latrunculia oparinae

Shkryl, Yuri N.; Bulgakov, Victor P.; Veremeichik, Galina N.; Kovalchuk, Svetlana N.; Kozhemyako, Valery B.; Kamenev, Dmitrii; Semiletova, I. V.; Timofeeva, Ya. O.; Shchipunov, Yu. A.; Kulchin, Yuri N.

doi:10.1007/s00449-015-1488-2

Cited by 14 publications

(6 citation statements)

References 14 publications

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“…It seems clear that many different phytochemicals could potentially function as both the reducing and stabilizing agents. Polysaccharides, proteins, and low-molecular weight secondary metabolites are commonly considered to be the major reducing and capping agents present in plant extracts [102,107,108].…”

Section: The Reducing Activity Of L Erythrorhizon Extract Componentsmentioning

confidence: 99%

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Shkryl

Rusapetova

Yugay

et al. 2021

IJMS

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The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), and bimetallic Ag/Au nanoparticles using the callus extract of Lithospermum erythrorhizon as a reducing and capping agent. The biosynthesized nanoparticles were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). Our results showed the formation of crystalline metal nanostructures of both spherical and non-spherical shape. Energy dispersive X-ray (EDX) spectroscopy showed the characteristic peaks in the silver and gold regions, confirming the presence of the corresponding elements in the monometallic particles and both elements in the bimetallic particles. Fourier-transform infrared (FTIR) spectroscopy affirmed the role of polysaccharides and polyphenols of the L. erythrorhizon extract as the major reducing and capping agents for metal ions. In addition, our results showed that the polysaccharide sample and the fraction containing secondary metabolites isolated from L. erythrorhizon were both able to produce large amounts of metallic nanoparticles. The biosynthesized nanoparticles demonstrated cytotoxicity against mouse neuroblastoma and embryonic fibroblast cells, which was considerably higher for Ag nanoparticles and for bimetallic Ag/Au nanoparticles containing a higher molar ratio of silver. However, fibroblast migration was not significantly affected by any of the nanoparticles tested. The obtained results provide a new example of the safe biological production of metallic nanoparticles, but further study is required to uncover the mechanism of their toxicity so that the biomedical potency can be assessed.

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Section: The Reducing Activity Of L Erythrorhizon Extract Componentsmentioning

confidence: 99%

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Shkryl

Rusapetova

Yugay

et al. 2021

IJMS

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“…lipolytica Po1h was used as the host strain for genetic manipulations aiming at the surface display of silicatein. The gene-encoding silicatein used in this work was derived from the SilA1 gene of the Latrunculia oparinae sponge. The coding sequence of the SilA1 gene, which was optimized according to the codon usage bias of Y.…”

Section: Methodsmentioning

confidence: 99%

“…Y. lipolytica Po1h 24 was used as the host strain for genetic manipulations aiming at the surface display of silicatein. The gene-encoding silicatein used in this work was derived from the SilA1 gene of the Latrunculia oparinae 49 sponge. The coding sequence of the SilA1 gene, which was optimized according to the codon usage bias of Y. lipolytica, was synthesized by Nanjing Jinsui Co. Ltd. (Nanjing, China), with an added histidine tag (6His), and was cloned into a pUC57 vector with added SfiI (at 5′ terminus) and BamHI (at 3′ terminus) restriction sites.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Genetical Surface Display of Silicatein on Yarrowia lipolytica Confers Living and Renewable Biosilica–Yeast Hybrid Materials

Wang

Liu

et al. 2020

ACS Omega

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In this work, a biological engineering-based biosilica–yeast hybrid material was developed. It was obtained by the aggregation of Yarrowia lipolytica through biosilicification catalyzed using genetically displayed silicatein on its cell surface. With orthosilicate or seawater as the substrate, the silicatein-displayed yeast could aggregate into flocs with a flocculation efficiency of nearly 100%. The resulting floc was found to be a sheetlike biosilica–yeast hybrid material formed by the biosilica-mediated immobilization of yeast cells via cross-linking and embedding, turning the original hydrophilicity of yeast cells into hydrophobicity. In addition, this material was characterized to be porous with an average pore diameter of approximately 10 μm and porosity of over 70%. Because of these properties, this hybrid material could achieve enhanced removal efficiencies for chromium ions and n-hexadecane, which were both above 99%, as compared to the free cells of Y. lipolytica in aqueous environments. Importantly, this hybrid material could be recultivated to generate new batches of yeast cells that maintain parallel properties to the first generation so that the same hybrid material could be reproduced with unchanged highly efficient removal of chromium and n-hexadecane to those of the first generation, demonstrating that this biosilica–yeast hybrid material was living and renewable. This work presented a novel way of harnessing silicatein and Y. lipolytica to achieve biological synthesis of a living inorganic–organic hybrid material that has potential to be applied in water treatment.

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“…During spicule formation in sponges, silicateins interact with few more proteins, such as collagen, galectin, and silintaphins, and form long fibers, which promote silica polymerization around them 3 . Silicatein-associated proteins have an impact on the silicatein activity 4 , but silicatein itself is sufficient to form amorphous 5 , 6 silica from different silica acid precursors in vitro .…”

Section: Introductionmentioning

confidence: 99%

Efficient silica synthesis from tetra(glycerol)orthosilicate with cathepsin- and silicatein-like proteins

Povarova

Barinov

Баранов

et al. 2018

Sci Rep

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Silicateins play a key role in biosynthesis of spicules in marine sponges; they are also capable to catalyze formation of amorphous silica in vitro. Silicateins are highly homologous to cathepsins L – a family of cysteine proteases. Molecular mechanisms of silicatein activity remain controversial. Here site-directed mutagenesis was used to clarify significance of selected residues in silica polymerization. A number of mutations were introduced into two sponge proteins – silicatein A1 and cathepsin L from Latrunculia oparinae, as well as into human cathepsin L. First direction was alanine scanning of the proposed catalytic residues. Also, reciprocal mutations were introduced at selected positions that differ between cathepsins L and silicateins. Surprisingly, all the wild type and mutant proteins were capable to catalyze amorphous silica formation with a water-soluble silica precursor tetra(glycerol)orthosilicate. Some mutants possessed several-fold enhanced silica-forming activity and can potentially be useful for nanomaterial synthesis applications. Our findings contradict to the previously suggested mechanisms of silicatein action via a catalytic triad analogous to that in cathepsins L. Instead, a surface-templated biosilification by silicateins and related proteins can be proposed.

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Bioinspired enzymatic synthesis of silica nanocrystals provided by recombinant silicatein from the marine sponge Latrunculia oparinae

Cited by 14 publications

References 14 publications

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Genetical Surface Display of Silicatein on Yarrowia lipolytica Confers Living and Renewable Biosilica–Yeast Hybrid Materials

Efficient silica synthesis from tetra(glycerol)orthosilicate with cathepsin- and silicatein-like proteins

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