Bioinspired Functionalization of Silica‐Encapsulated Yeast Cells

Yang, Sung Ho; Ko, Eun Hyea; Jung, Yang‐Il; Choi, Insung S.

doi:10.1002/anie.201102030

Cited by 96 publications

(67 citation statements)

References 55 publications

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“…The multilayer deposition was initiated with positively charged GO-NH þ 3 nanosheet to enhance the electrostatic interaction because the native yeast cells were known to present highly negative charge. [15][16][17] It is obvious that deposition of oppositely charged GO nanosheet would change the surface charge of the yeast cells. The surface charge, therefore, was individually measured with the progress of the deposition of GO-NH þ 3 and GO-COOnanosheets on the yeast cells from the first layer to the fifth layer ( Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

“…We, therefore, investigated the viability of yeast@GO by using FDA, which examined the activity of intracellular esterases and emitted green fluorescence by reactive oxygen species; yeast cells in green were considered alive, and the others were considered dead. [16,18,19] Viability test indicated that the GO nanosheet encapsulation was compatible with living yeast cells as shown in Figure 2. Moreover, the permeability to FDA implied that GO multilayer shell would be permeable to small molecules such as water or nutrients.…”

Section: Resultsmentioning

confidence: 99%

“…Organic polymers and inorganic nanoparticles can bring various new functionalities to the cell membranes, including fluorescent and magnetic property, catalytic moieties, and supporting templates. [11][12][13][14][15][16][17][18][19] For that, we have attempted to assemble multilayers on the yeast cells with a combination of GO nanosheets with two most common strong polyelectrolytes such as PDDA þ , and PSS -. According to the same protocol, the yeast cells were encapsulated based on the electrostatic interactions between oppositely charged GO nanosheets and organic polyelectrolytes in a format of yeast@(PDDA þ /GO-COO -) and yeast@(GO-NH þ 3 / PSS -), respectively.…”

Section: Resultsmentioning

confidence: 99%

“…[3][4][5][6][7][8][9] In addition, the surface of microbial cells was doped with various nanoparticles, such as silica, [10,11] gold, [12] silver, and iron, [13] and carbon nanotubes [14] by the LbL self-assembly. Very recently, individual yeast cells were encapsulated within silica, [15,16] calcium phosphate, [17] calcium carbonate, [18] and polydopamine, [19] inspired by biomineralization processes or an adhesive protein found in mussels. On the other hand, a new strategy to the modification of cell surfaces by in vivo synthesis of diverse nanoparticles has been reported for certain types of bacterial cells [20] or recombinant bacterial cells.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Yang

Lee

Seo

et al. 2011

Macromolecular Bioscience

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The first example of the encapsulation of living yeast cells with multilayers of GO nanosheets via LbL self‐assembly is reported. The GO nanosheets with opposite charges are alternatively coated onto the individual yeast cells while preserving the viability of the yeast cells, thus affording a means of interfacing graphene with living yeast cells. This approach is expanded by integrating other organic polymers or inorganic nanoparticles to the cells by hybridizing the entries with GO nanosheets through LbL self‐assembly. It is demonstrated that incorporated iron oxide nanoparticles can deliver magnetic properties to the biological systems, allowing the integration of new physical and chemical functions for living cells with a combination of GO nanosheets.magnified image

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Yang

Lee

Seo

et al. 2011

Macromolecular Bioscience

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“…Our synthetic approach could be applied to the interdisciplinary fields, specifically related to living cells, such as nanobiotechnology, biomedical applications, and cell-based nano/micro devices, because these applications require the cytocompatible process to integrate low cytotoxic materials with cell surfaces. [19][20][21][22] In addition, the silicification carried Notes out in the synergistic fashion could be another tool for investigating silicification mechanisms in nature.…”

mentioning

confidence: 99%

Bioinspired Fabrication of Silica Thin Films on Histidine-Terminated Self-Assembled Monolayers

Park¹,

Lee²,

Choi³

et al. 2014

Bulletin of the Korean Chemical Society

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There exists a great deal of interest in silica thin films, which potentially allow us to develop various applications including heterogeneous catalysis, 1 wettability control, 2 and microelectronics 3 because of their unique physicochemical properties, such as transparency, mechanical hardness, chemical stability, and low dielectric constant. Recently, the scope of substrates, on which silica thin films are deposited, has been expanded to biological entities for exploiting the emerging fields of drug delivery, 4 cell culture, 5 biosensors, and cell encapsulation. 7-9 Although chemical deposition and thermal treatment have been considered as a simple way to fabricate silica thin films, toxic chemicals and extreme temperature are required for silica-forming processes. 10In contrast to the chemical and thermal methods, biosilicification occurring in diatoms and glass sponges has its own characteristic strategies to concentrate a small amount of silicic acid from sea water and construct the siliceous exoskeletons under mild conditions (e.g., neutral pH, room temperature, aqueous media).11,12 Despite the similarity in materials, it is known that silica-forming mechanisms in the two organisms are completely different. The biosilicification in diatoms is achieved by silaffins, highly phosphorylated silica-forming peptides extracted from the cell wall of diatoms, where ε-amino group in lysine residues are posttranslationally modified to ε-N-dimethyllysine or ε-N,N,Ntrimethyl-δ-hydroxylysine or a long-chain polyamine comprising of a few N-methylpropylamine units.11 The selfassembled structure of the cationic polypeptides in silaffins is believed to act as a catalytic template for the in vivo polycondensation of silicic acid derivatives. Inspired by silaffins, cationic synthetic polymers and polypeptides have been used as catalytic templates for fabricating silica thin films. For example, silica thin films were formed on a gold substrate by using poly((2-dimethylamino)ethyl methacrylate) (PDMAEMA) grown by surface-initiated polymerization.13,14 The positively charged PDMAEMA could attract the negatively charged silicic acids, followed by the polycondensation to silica. In a similar fashion, polyelectrolyte multilayers of poly(diallyldimethylammonium chloride) (PDADMA), formed by the layer-by-layer method, were used as a catalytic template for generating micro-patterned silica films. 15On the other hand, silicateins, silica-forming proteins extracted from the spicules of various kinds of glass sponges, catalyze the polycondensation of silicic acids by mainly using two specific amino acids: histidine and serine that are in close proximity for hydrogen bonding.12 Because of the hydrogen bonding, the nucleophilic attack of oxygen in serine to silicon alkoxide is believed to occur effectively. In contrast to silaffins, the silicatein-catalyzed formation of silica thin films was carried out with silicon alkoxide at pH 7.4. For example, Perry et al. reported the formation of uniform silica thin films by immobilizing recombinant...

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Biomimetic Coatings to Control Cellular Function through Cell Surface Engineering

Drachuk

Gupta

Tsukruk

2013

Adv Funct Materials

111

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Biologically inspired materials and structures with tailored biological, physical, and chemical properties provide an indispensable platform to actively modulate and protect cell function in hostile and synthetic environments (e.g., devices or matrices). Herein, recent innovations are discussed in cell surface engineering methods based on bulk hydrogels, microgels, and ultrathin capsules derived from inorganic, polymeric, biomolecular, or nanoparticle materials applicable to various protective and interaction mediating applications. These biomimetic cell coatings can dramatically increase cell viability and stability in a hostile environment and expand their applicability for demanding biomedical, biotechnology, and bioelectronics applications. Some of the most recent studies of traditional inorganic and organic gels, polymeric and biomolecular microgels, and ultrathin conformal soft shells from polymers and proteins are also discussed. Proper selection of chemical composition and assembly conditions has potential to dramatically enhance viability of encapsulated cells by increasing their mechanical stability, masking the cell surface from immunological agents, increasing mechanical stability, and providing chemical resistance to aggressive environments. Some recent examples of such robust and viable protected cells for biotechnology and bioelectronics are presented.

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Bioinspired Functionalization of Silica‐Encapsulated Yeast Cells

Cited by 96 publications

References 55 publications

Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Bioinspired Fabrication of Silica Thin Films on Histidine-Terminated Self-Assembled Monolayers

Biomimetic Coatings to Control Cellular Function through Cell Surface Engineering

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