Bioinspired short peptide hydrogel for versatile encapsulation and controlled release of growth factor therapeutics

Hiew, Shu Hui; Wang, Jun Kit; Koh, Kenrick; Yang, Hai‐Bo; Bacha, Abbas; Lin, Junquan; Yip, Yun Sheng; Vos, Marcus Ivan Gerard; Chen, Liyan; Sobota, Radoslaw M.; Tan, Nguan Soon; Tay, Chor Yong; Miserez, Ali

doi:10.1016/j.actbio.2021.09.023

Cited by 26 publications

(28 citation statements)

References 59 publications

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“…A short peptide (GV8) hydrogel can self-assemble and maintain the activity of delivery cells under mild physiological conditions. GV8 peptide hydrogel containing ADSCs secretome has been shown to have good potential in full-thickness excisional wound healing C57/BL6 mice model ( Hiew et al, 2021 ). Here, ADSCs secrete factors that regulate angiogenesis and endothelial cell migration (thymosin beta 4 X-linked [TMSB4X], gremlin 1 [GREM1], and extracellular matrix protein 1 [ECM1]), metalloproteinase inhibition (tissue inhibitors of metalloproteinases 1 [TIMP1]), cell proliferation (EGF containing fibulin extracellular matrix protein 1 [EFEMP1]), immune cell proliferation and morphogenesis (colony-stimulating factor 1 [CSF1]), and growth factor interaction (latent transforming growth factor-beta binding protein 1 [LTBP1]) ( Hiew et al, 2021 ).…”

Section: The Application Of Hydrogel Combined With Stem Cells In the ...mentioning

confidence: 99%

“…GV8 peptide hydrogel containing ADSCs secretome has been shown to have good potential in full-thickness excisional wound healing C57/BL6 mice model ( Hiew et al, 2021 ). Here, ADSCs secrete factors that regulate angiogenesis and endothelial cell migration (thymosin beta 4 X-linked [TMSB4X], gremlin 1 [GREM1], and extracellular matrix protein 1 [ECM1]), metalloproteinase inhibition (tissue inhibitors of metalloproteinases 1 [TIMP1]), cell proliferation (EGF containing fibulin extracellular matrix protein 1 [EFEMP1]), immune cell proliferation and morphogenesis (colony-stimulating factor 1 [CSF1]), and growth factor interaction (latent transforming growth factor-beta binding protein 1 [LTBP1]) ( Hiew et al, 2021 ). In addition, platelet-rich plasma hydrogel can provide a stable physical framework and promote the potential of ADSCs in vitro and in vivo , increasing their expression of ANGPT1 and angiopoietin 2 (ANGPT2) and promoting male athymic rats wound healing ( Samberg et al, 2019 ).…”

Section: The Application Of Hydrogel Combined With Stem Cells In the ...mentioning

confidence: 99%

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Advances of hydrogel combined with stem cells in promoting chronic wound healing

Wang

Jiang

et al. 2022

Front. Chem.

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Wounds can be divided into two categories, acute and chronic. Acute wounds heal through the normal wound healing process. However, chronic wounds take longer to heal, leading to inflammation, pain, serious complications, and an economic burden of treatment costs. In addition, diabetes and burns are common causes of chronic wounds that are difficult to treat. The rapid and thorough treatment of chronic wounds, including diabetes wounds and burns, represents a significant unmet medical need. Wound dressings play an essential role in chronic wound treatment. Various biomaterials for wound healing have been developed. Among these, hydrogels are widely used as wound care materials due to their good biocompatibility, moisturizing effect, adhesion, and ductility. Wound healing is a complex process influenced by multiple factors and regulatory mechanisms in which stem cells play an important role. With the deepening of stem cell and regenerative medicine research, chronic wound treatment using stem cells has become an important field in medical research. More importantly, the combination of stem cells and stem cell derivatives with hydrogel is an attractive research topic in hydrogel preparation that offers great potential in chronic wound treatment. This review will illustrate the development and application of advanced stem cell therapy-based hydrogels in chronic wound healing, especially in diabetic wounds and burns.

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Section: The Application Of Hydrogel Combined With Stem Cells In the ...mentioning

confidence: 99%

Section: The Application Of Hydrogel Combined With Stem Cells In the ...mentioning

confidence: 99%

Advances of hydrogel combined with stem cells in promoting chronic wound healing

Wang

Jiang

et al. 2022

Front. Chem.

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“…The proteome list was obtained from ref. [42]. Preparation and analysis of the secretome using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were performed as described in ref.…”

Section: Secretome Proteomics Analysismentioning

confidence: 99%

“…Preparation and analysis of the secretome using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were performed as described in ref. [42]. The resultant protein list was filtered by first using the Gene Ontology (GO) cellular component database to identify the extracellular proteins, followed by the removal of contaminants (FBS, keratin from the environment, etc.).…”

Section: Secretome Proteomics Analysismentioning

confidence: 99%

Squid Suckerin‐Spider Silk Fusion Protein Hydrogel for Delivery of Mesenchymal Stem Cell Secretome to Chronic Wounds

Koh

Wang

Yuan

et al. 2022

Adv Healthcare Materials

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Chronic wounds are non‐healing wounds characterized by a prolonged inflammation phase. Excessive inflammation leads to elevated protease levels and consequently to a decrease in growth factors at wound sites. Stem cell secretome therapy has been identified as a treatment strategy to modulate the microenvironment of chronic wounds via supplementation with anti‐inflammatory/growth factors. However, there is a need to develop better secretome delivery systems that are able to encapsulate the secretome without denaturation, in a sustained manner, and that are fully biocompatible. To address this gap, a recombinant squid suckerin‐spider silk fusion protein is developed with cell‐adhesion motifs capable of thermal gelation at physiological temperatures to form hydrogels for encapsulation and subsequent release of the stem cell secretome. Freeze–thaw treatment of the protein hydrogel results in a modified porous cryogel that maintains slow degradation and sustained secretome release. Chronic wounds of diabetic mice treated with the secretome‐laden cryogel display increased wound closure, presence of endothelial cells, granulation wound tissue thickness, and reduced inflammation with no fibrotic scar formation. Overall, these in vivo indicators of wound healing demonstrate that the fusion protein hydrogel displays remarkable potential as a delivery system for secretome‐assisted chronic wound healing.

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“…To date, various types of materials (e.g., natural protein, polysaccharide, DNA, peptide, as well as synthetic polyethylene glycol (PEG) derivatives) have been designed and engineered for regulating cell biology, further targeting different biomedical applications. [6][7][8] As is well-documented, these materials are able to affect cell biology via single or multiple interactions. Thus, versatile materials with multifunctionality are usually highly preferred.…”

Section: Introductionmentioning

confidence: 99%

Versatile polyphenolic platforms in regulating cell biology

et al. 2022

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Bioinspired short peptide hydrogel for versatile encapsulation and controlled release of growth factor therapeutics

Cited by 26 publications

References 59 publications

Advances of hydrogel combined with stem cells in promoting chronic wound healing

Advances of hydrogel combined with stem cells in promoting chronic wound healing

Squid Suckerin‐Spider Silk Fusion Protein Hydrogel for Delivery of Mesenchymal Stem Cell Secretome to Chronic Wounds

Versatile polyphenolic platforms in regulating cell biology

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