Bioinspired Synthesis of All‐in‐One Organic–Inorganic Hybrid Nanoflowers Combined with a Handheld pH Meter for On‐Site Detection of Food Pathogen

Ye, Ranfeng; Zhu, Chengzhou; Yang, Song; Lu, Qian; Ge, Xiaoxiao; Yang, Xu; Zhu, Mei; Du, Dan; He, Li; Lin, Yuehe

doi:10.1002/smll.201600273

Cited by 135 publications

(40 citation statements)

References 38 publications

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“…Representatively, glucose oxidase (GOx) and horseradish peroxidase (HRP) were employed as two different enzyme components with copper sulfate in phosphate buffered saline (PBS), resulting in multienzyme incorporated hybrid nanoflowers, which can be utilized for detecting target glucose via the GOx‐HRP mediated cascade enzyme reaction . Furthermore, another type of organic components, such as antibody, streptavidin, or concanavalin A, all of which have high affinity for their corresponding target molecules, were added to generally used enzymes and inorganic metal ions, to develop triple component‐incorporated hybrid nanoflowers. The nanoflowers described above were proven to have multifunctionalities composed of biorecognition and signal amplification, which were arisen from the entrapped biomolecules, including antibody, streptavidin or concanavalin A, mediating specific recognition and the enzymes catalyzing signaling, respectively.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Cheon

Adhikari

Chung

et al. 2019

Adv Healthcare Materials

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It is reported that glucose oxidase (GOx)‐copper hybrid nanoflowers embedded with Fe3O4 magnetic nanoparticles (MNPs) exhibit superior peroxidase‐mimicking activity as well as substrate channeling for glucose detection. This is due to the synergistic integration of GOx, crystalline copper phosphates and MNPs being in close proximity within the nanoflowers. The preparation of MNP‐embedded GOx‐copper hybrid nanoflowers (MNPs‐GOx NFs) begins with the facile conjugation of amine‐functionalized MNPs with GOx molecules via electrostatic attraction, followed by the addition of copper sulfate that leads to full blooming of the hybrid nanoflowers. In the presence of glucose, the catalytic action of GOx entrapped in the nanoflowers generates H2O2, which is subsequently used by peroxidase‐mimicking MNPs and copper phosphate crystals, located close to GOx molecules, to convert Amplex UltraRed substrate into a highly fluorescent product. Using this strategy, the target glucose is successfully determined with excellent selectivity, stability, and magnetic reusability. This biosensor based on hybrid nanoflowers also exhibits a high degree of precision and reproducibility when applied to real human blood samples. Such novel MNP‐embedded enzyme‐inorganic hybrid nanoflowers have a great potential to be expanded to any oxidases, which will be highly beneficial for the detection of various other clinically important target molecules.

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Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Cheon

Adhikari

Chung

et al. 2019

Adv Healthcare Materials

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“…And then, it was used for a portable sensitive ELISA detection of Escherichia coli O157:H7. Under the optimal conditions, the detection sensitivity can reach as low as 10 CFU/mL for the case of concanavalin A-glucose oxidase [17] and 60 CFU/mL for the case of antibody-HRP [35].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

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“…Thanks to E. coli O157:H7 recognition of Con A and hydrolysis of sucrose to glucose by invertase, they succeeded point-of-care detection of E. coli O157:H7 from milk by measuring glucose amount. Same group also made very similar detection of food pathogen by synthesizing Con A-GOx organic-inorganic hybrid nanoflowers [54]. They, this time, used GOx as signal amplification unit to convert glucose to gluconic acid resulting pH decrease which can be measured by portable pH meter.…”

Section: Multi-enzyme Incorporated Fhnsmentioning

confidence: 99%

Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications

Tasdemir¹,

Çelik²,

Demırbas³

et al. 2018

Preprint

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A recent breakthrough in preparation of immobilized enzyme based biocatalysts achieving highly enhanced enzymatic activities and stabilities has become a great alternative to conventional immobilization techniques. The functional hybrid nanobiocatalysts (FHNs) fabricated in this immobilization composed of organic components (amino acid, peptide, protein, enzyme and plant extract) and inorganic components (various metal ions) give flowerlike morphology with narrow size distribution and porous structure. The enzyme incorporated FHNs exhibite greatly enhanced catalytic activities and stabilities compared to free and conventionally immobilized enzymes under various experimental conditions. In addition to that, the FHNs consisting of other organic components act as Fenton-like reagents and show peroxidase-like activity owing to presence of metal ions and porous structure in the FHNs. This report basically focuses on preparation, characterization, and bioanalytical applications of the FHNs and explain mechanism of the FHNs formation and thier enhanced activities and stabilities.Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Bioinspired Synthesis of All‐in‐One Organic–Inorganic Hybrid Nanoflowers Combined with a Handheld pH Meter for On‐Site Detection of Food Pathogen

Cited by 135 publications

References 38 publications

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Magnetic Nanoparticles‐Embedded Enzyme‐Inorganic Hybrid Nanoflowers with Enhanced Peroxidase‐Like Activity and Substrate Channeling for Glucose Biosensing

Current and Emerging Technologies for Rapid Detection of Pathogens

Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications

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