Biointerface topography mediates the interplay between endothelial cells and monocytes

Liu, Yan; Deng, Wenshuai; Yang, Liangliang; Fu, Xiuxiu; Wang, Zhibin; Rijn, Patrick van; Zhou, Qihui; Yu, Tao

doi:10.1039/d0ra00704h

Cited by 8 publications

(9 citation statements)

References 35 publications

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“…The adhesion and migration of monocytes to endothelial cells is a process of the inflammatory response, which is mediated by specific molecules on endothelial cells and monocytes ( Ross, 1999 ; Bian et al, 2017 ; Lin et al, 2018 ; Liu et al, 2020 ). THP-1 cells were seeded on HUVECs exposed to different materials.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Chen

Zhu

Hao

et al. 2021

Front. Bioeng. Biotechnol.

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Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels.

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Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Chen

Zhu

Hao

et al. 2021

Front. Bioeng. Biotechnol.

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“…5 and S2). Overall, P-selectin, E-selectin and ICAM-1 are correlated with the promotion of cell adhesion, in this particular case, to the implantable material, and the other analyzed markers are associated with the interplay of immune cells to develop an immune response [21,38,39]. Of the 20 analyzed markers, 16 of them are typically associated with the macrophage's M1-like phenotype and the remaining 4 (IL-4 [34], IL-10 [34], IL-13 and IFN-α) are related to macrophage's M2-like phenotype [39].…”

Section: Discussionmentioning

confidence: 86%

“…Aiming to assess the macrophages' response to the selected surface topographies, we selected a model based on dTHP-1 that is a valid model to predict the host response in vivo [1,6,7,[21][22][23]. dTHP-1 do not present significant differences in its morphology when cultured on top of all analyzed substrates.…”

Section: Discussionmentioning

confidence: 99%

“…The goal of this study was to assess the macrophages' response to the selected natural surface topographies. For that, we selected a model based on THP-1-derived macrophages (dTHP-1) that is widely regarded as a valid model to investigate biomaterials, particularly due to its demonstrated ability to relate to the host response in vivo [1,6,7,[21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic surface topography as a potential modulator of macrophages inflammatory response to biomaterials

Monteiro

Casanova

Quinteira

et al. 2022

Biomaterials Advances

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“…The presence of micro- and nano-textured surface topography is known to promote both adhesion and proliferation of HUVECs [ 110 ]. On the other hand, effective cellular adhesion was observed on un-patterned micron-rough substrates as well as grooved and wrinkled substrates [ 111 , 112 ]. Since laser surface texturing is an effective approach for micro-patterning of the surface of different materials, it is gaining huge potential in biomedical applications requiring micron-rough substrate for cell adhesion [ 113 ].…”

Section: Resultsmentioning

confidence: 99%

Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale

Perrone

Cesaria

Zizzari

et al. 2021

Materials Today Bio

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Carbon dioxide (CO 2 )-laser processing of glasses is a versatile maskless writing technique to engrave micro-structures with flexible control on shape and size. In this study, we present the fabrication of hundreds of microns quartz micro-channels and micro-holes by pulsed CO 2 -laser ablation with a focus on the great potential of the technique in microfluidics and biomedical applications. After discussing the impact of the laser processing parameters on the design process, we illustrate specific applications. First, we demonstrate the use of a serpentine microfluidic reactor prepared by combining CO 2 -laser ablation and post-ablation wet etching to remove surface features stemming from laser-texturing that are undesirable for channel sealing. Then, cyclic olefin copolymer micro-pillars are fabricated using laser-processed micro-holes as molds with high detail replication. The hundreds of microns conical and square pyramidal shaped pillars are used as templates to drive 3D cell assembly. Human Umbilical Vein Endothelial Cells are found to assemble in a compact and wrapping way around the micro-pillars forming a tight junction network. These applications are interesting for both Lab-on-a-Chip and Organ-on-a-Chip devices.

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Biointerface topography mediates the interplay between endothelial cells and monocytes

Abstract: This study offers a new in vitro system to understand the interplay between HUVEC monolayer and monocytes mediated by aligned topographies, which may be useful for vascular repair and disease modeling for drug testing.

Cited by 8 publications

References 35 publications

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Biomimetic surface topography as a potential modulator of macrophages inflammatory response to biomaterials

Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale

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