Biokompatible funktionalisierte zellgängige Polyglycerinmikrogele

Abstract

Gel für die Zelle: Eine neue Methode zur Herstellung biokompatibler Polyglycerinmikrogele mit einstellbaren Größen (25–100 nm; oben im Bild) und Oberflächenfunktionalisierung (rote Kugeln) wurde entwickelt. Fluoreszenzmikroskopische Messungen belegen, dass die Partikel durch endocytotische Mechanismen in die Zellen aufgenommen und intrazellulär in der perinuklearen Region lokalisiert werden (unten im Bild).

Polyglycerol‐Tagged Molecular Clips as Receptors in Protic Solvents

“…For this purpose, we synthesized four clips modified with polyglycerol (PG) dendrons (2 and 3) of first and second generation ([G1.0] and [G2.0]) and with dendritic hyperbranched polyglycerols (HPG) (4 and 5) in the central unit as solubilizing units ( Figure 2). [5] In contrast to the perfectly branched glycerol dendrimers and dendrons, which have a degree of branching (DB) of 100 %, HPG is randomly branched but has a well-defined dendritic structure with a DB of 60 to 75 %. [4] Hyperbranched polyglycerols are "dendrimer like" polymers that exhibit good biocompatibility and are well-soluble in polar solvents.…”

“…PG dendrons have a unique design that is based on the extremely biocompatible glycerol building block. (3), HPG (1kDa) (4), and HPG (3kDa) (5). [5] In contrast to the perfectly branched glycerol dendrimers and dendrons, which have a degree of branching (DB) of 100 %, HPG is randomly branched but has a well-defined dendritic structure with a DB of 60 to 75 %.…”

“…The maximum complexationinduced 1 H NMR shifts, Δδ max , of the guest protons, the association constants, K a , and, hence, the Gibbs enthalpies of association, ΔG, were determined by 1 H NMR titration experiments, in which Δδ max is defined as the difference between the chemical 1 H NMR shifts of the signals assigned to the free and complexed guest (δ 0 -δ C ). However, the different curvature in the plots of the ob- (3), HPG (1kDa) (4), and HPG (3kDa) (5). [3c,3e,3g] In the case of molecular clips 4 and 5 substituted by the hyperbranched polyglycerols, which do not consist of single structures, averages of the molecular mass distribution were used to calculate the host concentrations and, hence, the K a and Δδ max values from the dependence of the complexation-induced 1 H NMR shifts Δδ obs (= δ obs -δ 0 ) on the host concentration at constant guest concentration.…”

Makromolekulare Chemie 2009

“…Mit dem Konzept lassen sich über inverse Miniemulationspolymerisation durch säurekatalysierte Polyaddition hochmolekulare Polyglycerinmikrogele mit bisher nicht erreichten Durchmessern von bis zu 80 nm und engen Größenverteilungen erzeugen 27. Auch diese sind nachträglich über z.…”

Functionalized Polyglycerol Amine Nanogels as Nanocarriers for DNA

“…Additionally, nPG show a high Young's modulus which is a sign of a rigid core structure, with the result that they have been much studied for their stiffness and size . In the search for new polymeric architectures that have a longer circulation half life in blood, the synthesis of different nPGs and studies of their physicochemical properties have been reported . In this paper, we synthesize polyglycerol amine nanogels and study the relation of charge density, their behavior during polyplex formation with DNA and cellular uptake.…”