Biolistic introduction of a synthetic Bt gene into elite maize

Hill, Martha; Launis, Karen; Bowman, Cindy; McPherson, K.; Dawson, John F.; Watkins, J.; Koziel, Michael G.; Wright, Martha

doi:10.1007/bf00023939

Cited by 12 publications

(3 citation statements)

References 16 publications

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“…Bt MON810 maize is resistant to attack by Lepidopterae pests, as it expresses the Cry1Ab protein [5] . This protein is produced in all plant tissues as a result of introduction of the bacterial cry1Ab transgene into the maize genome [6] . While the bacterial Cry1Ab protein has been extensively used as an organic insecticide [7] , its expression in transgenic maize could potentially alter its structure which may render it allergenic or otherwise harmful upon ingestion [8] .…”

Section: Introductionmentioning

confidence: 99%

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

et al. 2012

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BackgroundWe aimed to determine the effect of feeding transgenic maize to sows during gestation and lactation on maternal and offspring immunity and to assess the fate of transgenic material.Methodology/Principal FindingsOn the day of insemination, sows were assigned to one of two treatments (n = 12/treatment); 1) non-Bt control maize diet or 2) Bt-MON810 maize diet, which were fed for ∼143 days throughout gestation and lactation. Immune function was assessed by leukocyte phenotyping, haematology and Cry1Ab-specific antibody presence in blood on days 0, 28 and 110 of gestation and at the end of lactation. Peripheral-blood mononuclear cell cytokine production was investigated on days 28 and 110 of gestation. Haematological analysis was performed on offspring at birth (n = 12/treatment). Presence of the cry1Ab transgene was assessed in sows' blood and faeces on day 110 of gestation and in blood and tissues of offspring at birth. Cry1Ab protein presence was assessed in sows' blood during gestation and lactation and in tissues of offspring at birth. Blood monocyte count and percentage were higher (P<0.05), while granulocyte percentage was lower (P<0.05) in Bt maize-fed sows on day 110 of gestation. Leukocyte count and granulocyte count and percentage were lower (P<0.05), while lymphocyte percentage was higher (P<0.05) in offspring of Bt maize-fed sows. Bt maize-fed sows had a lower percentage of monocytes on day 28 of lactation and of CD4+CD8+ lymphocytes on day 110 of gestation, day 28 of lactation and overall (P<0.05). Cytokine production was similar between treatments. Transgenic material or Cry1Ab-specific antibodies were not detected in sows or offspring.Conclusions/SignificanceTreatment differences observed following feeding of Bt maize to sows did not indicate inflammation or allergy and are unlikely to be of major importance. These results provide additional data for Bt maize safety assessment.

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Section: Introductionmentioning

confidence: 99%

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

et al. 2012

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“…Many of the world's most important crops have already been successfully genetically engineered, including wheat (He et al, 1994), rice (Christou et al, 1991;Hiei et al, 1994), barley (Ritala et al, 1994;Wan and Lemaux, 1994), maize (Gordon-Kamm et al, 1990;Hill et al, 1995) and sugarcane (Bower and Birch, 1992). Herbicide tolerance (De Block et al, 1987;Vasil et al, 1992), altered oil composition in seeds (Knutzon et al, 1992), and increased resistance to insects (Meeusen and Warren, 1989;Koziel et al, 1993) and diseases (Lomonossof, 1995) are a selection of traits that have already been integrated into plant genomes.…”

Section: Introductionmentioning

confidence: 99%

The investigation of optimal bombardment parameters for transient and stable transgene expression in Sorghum

Able

Rathus

Godwin

2001

In Vitro Cell.Dev.Biol.-Plant

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This report outlines the development of optimized particle inflow gun (PIG) parameters for producing transgenic sorghum (Sorghum bicolor (L.) Moench). Both transient and stable expression were examined when determining these parameters. The uidA reporter gene (GUS) encoding b-glucuronidase was used in transient experiments and the green fluorescent protein (GFP) used to monitor stable expression. Initially, optimization was conducted using leaf segments, as the generation of sorghum callus in sufficiently large quantities is time-consuming. Following leaf optimization, experiments were conducted using callus, identifying a high similarity between the two tissue types r s 0X83X High levels of GUS expression were observed in both leaf and callus material when most distant from the DNA expulsion point, and using a pressure greater than 1800 kPa. A higher level of expression was also observed when the aperture of the helium inlet valve was constricted. Using the optimized conditions (pressure of 2200 kPa, distance to target tissue of 15 cm from the expulsion point, and the aperture of the helium inlet valve at one full turn), three promoters (Ubiquitin, Actin1 and CaMV 35S) were evaluated over a 72-h period using GUS as the reporter gene. A significantly higher number of GUS foci were counted with the Ubiquitin construct over this period, compared to the Actin1 and CaMV 35S constructs. Stable callus sectors (on 2 mg l 21 bialaphos) with GFP expression were visualized for as long as 6 wk post-bombardment. Using this optimized protocol, several plants were regenerated after having been bombarded with the pAHC20 construct (containing the bar gene), with molecular evidence confirming integration.

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“…The genetically modified (GM) maize event MON810 expresses a Bacillus thuringiensis -derived gene, namely, a truncated cry1Ab gene encoding an insecticidal protein (δ-endotoxin; Schnepf et al 1998), to control some lepidopteran insect pests such as the European corn borer ( Ostrinia nubilalis ; Hill et al 1995). Concerns regarding potential adverse health effects following the ingestion of the MON810 maize have been raised, and it has been claimed that the immune system in Atlantic salmon (Sagstad et al 2007; Gu et al 2013), mice (Finamore et al 2008; Adel-Patient et al 2011) and pigs (Walsh et al 2011) may be affected following the oral/intragastric administration of the MON810 maize.…”

Section: Introductionmentioning

confidence: 99%

Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE)

et al. 2018

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The genetically modified maize event MON810 expresses a Bacillus thuringiensis-derived gene, which encodes the insecticidal protein Cry1Ab to control some lepidopteran insect pests such as the European corn borer. It has been claimed that the immune system may be affected following the oral/intragastric administration of the MON810 maize in various different animal species. In the frame of the EU-funded project GRACE, two 90-day feeding trials, the so-called studies D and E, were performed to analyze the humoral and cellular immune responses of male and female Wistar Han RCC rats fed the MON810 maize. A MON810 maize variety of Monsanto was used in the study D and a MON810 maize variety of Pioneer Hi-Bred was used in the study E. The total as well as the maize protein- and Cry1Ab-serum-specific IgG, IgM, IgA and IgE levels, the proliferative activity of the lymphocytes, the phagocytic activity of the granulocytes and monocytes, the respiratory burst of the phagocytes, a phenotypic analysis of spleen, thymus and lymph node cells as well as the in vitro production of cytokines by spleen cells were analyzed. No specific Cry1Ab immune response was observed in MON810 rats, and anti-maize protein antibody responses were similar in MON810 and control rats. Single parameters were sporadically altered in rats fed the MON810 maize when compared to control rats, but these alterations are considered to be of no immunotoxicological significance.Electronic supplementary materialThe online version of this article (10.1007/s00204-018-2230-z) contains supplementary material, which is available to authorized users.

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Biolistic introduction of a synthetic Bt gene into elite maize

Cited by 12 publications

References 16 publications

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

Effects of Feeding Bt Maize to Sows during Gestation and Lactation on Maternal and Offspring Immunity and Fate of Transgenic Material

The investigation of optimal bombardment parameters for transient and stable transgene expression in Sorghum

Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE)

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