Biologia molecular aplicada às dermatoses tropicais

Roselino, Ana Maria Ferreira

doi:10.1590/s0365-05962008000300002

Cited by 8 publications

(8 citation statements)

References 55 publications

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“…PCR allows detecting slow growth or uncultivable microorganisms, and, based on the available genetic data, has been used to detect M. leprae , since 1989. 58 - 60 …”

Section: Laboratory Diagnosismentioning

confidence: 99%

“…It is based on the amplification of specific sequences of M. leprae genome and in the identification of the fragment of amplified deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). 60 However, this technique is limited to research centers, due to the high cost of reagents and to the need of specific equipment and qualified professionals.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

“…Several factors may interfere with detection rates, such as the different primers aiming at different genomic targets of M. leprae , the size of amplified fragments, and the amplification technique used in the investigation are. 60 . 61 …”

Section: Laboratory Diagnosismentioning

confidence: 99%

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Leprosy: a review of laboratory and therapeutic aspects - Part 2

Lastória

Abreu

2014

An. Bras. Dermatol.

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Leprosy is a chronic infectious condition caused by Mycobacterium leprae(M. leprae). It is endemic in many regions of the world and a public health problem in Brazil. Additionally, it presents a wide spectrum of clinical manifestations, which are dependent on the interaction between M. leprae and host, and are related to the degree of immunity to the bacillus. The diagnosis of this disease is a clinical one. However, in some situations laboratory exams are necessary to confirm the diagnosis of leprosy or classify its clinical form. This article aims to update dermatologists on leprosy, through a review of complementary laboratory techniques that can be employed for the diagnosis of leprosy, including Mitsuda intradermal reaction, skin smear microscopy, histopathology, serology, immunohistochemistry, polymerase chain reaction, imaging tests, electromyography, and blood tests. It also aims to explain standard multidrug therapy regimens, the treatment of reactions and resistant cases, immunotherapy with bacillus Calmette-Guérin (BCG) vaccine and chemoprophylaxis.

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“…PCR allows detecting slow growth or uncultivable microorganisms, and, based on the available genetic data, has been used to detect M. leprae , since 1989. 58 - 60 …”

Section: Laboratory Diagnosismentioning

confidence: 99%

Section: Laboratory Diagnosismentioning

confidence: 99%

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Leprosy: a review of laboratory and therapeutic aspects - Part 2

Lastória

Abreu

2014

An. Bras. Dermatol.

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“…Esse método vem sendo hoje aplicado, frequentemente, em pesquisas de doenças infecciosas de variada etiologia (SANTOS et al, 2007). Em relação à investigação de dermatoses tropicais, em medicina humana, muito se tem adquirido com a utilização da biologia molecular, sendo que o objetivo mais atual se volta para o auxílio no desenvolvimento de fármacos e de vacinas para o seu controle, em especial no caso da hanseníase (ROSELINO, 2008).…”

Section: Discussionunclassified

Diagnóstico molecular do granuloma lepróide canino, a partir de cortes histológicos emblocados em parafina, pela técnica de reação em cadeia de polimerase (Pcr) – estudo retrospectivo (2002-2009)

et al. 2015

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O Granuloma lepróide canino (GLC) ou “lepra canina” consiste em micobacteriose, com quadro dermatopático que conta com aspectos ainda obscuros, principalmente quanto ao seu modo de transmissão e agente etiológico envolvido. Com a finalidade de se verificar a caracterização gênica da micobactéria em questão, procedeu-se estudo a partir de cortes histológicos, de animais com diagnóstico estabelecido da enfermidade. A amostragem foi composta de 13 cães, atendidos no Serviço de Dermatologia do HOVET/USP. Tal material foi posteriormente submetido às técnicas de reação em cadeia de polimerase (PCR), “nested” e em tempo real. O método da PCR “nested” confirmou a presença do agente em apenas 18,7% dos animais. Enquanto que, em 69,2% das amostras, o DNA da micobactéria envolvida foi detectado pela PCR em tempo real, demonstrando ser neste estudo, o método de maior sensibilidade. Pôde-se concluir ainda que a espécie de micobactéria causadora do Granuloma lepróide canino nos cães brasileiros possui homologia gênica de 100%, quando comparada às amostras provindas de animais de estudos estrangeiros.

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“…Optimizing a real-time PCR reaction using primers and probes in this region may improve mean sensitivity rates and also increase detection of resistant forms in multiplex assays. 23,24,25 Nevertheless, each case should clearly be screened using a wide portfolio of methods in view of the increasing capacity of these techniques to reach a diagnosis. For example, PCR together with culture in Lowenstein-Jensen medium and Ziehl-Neelsen staining was able to detect one case that was misdiagnosed at histopathology.…”

Section: Discussionmentioning

confidence: 99%

HIV-associated tuberculous lymphadenitis: the importance of polymerase chain reaction (PCR) as a complementary tool for the diagnosis of tuberculosis - a study of 104 patients

Cortez

Oliveira

Monte

et al. 2011

An. Bras. Dermatol.

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BACKGROUND: Lymphadenitis is common in HIV-positive patients. Diagnosis of the infections associated with this condition is complex, particularly in the case of tuberculosis. Rapid and specific detection of Mycobacterium tuberculosis (M. tuberculosis) is fundamental in ensuring adequate treatment. In addition, frequent causes of lymphadenitis such as those associated with lymphoma and histoplasmosis, among others, must be eliminated as possible causes. OBJECTIVES: To evaluate the accuracy of polymerase chain reaction as a tool for the diagnosis of lymphadenitis resulting from M. tuberculosis. METHODS: In this study, a protocol was developed using the following procedures: direct microscopy using Ziehl-Neelsen staining, culture in Lowenstein-Jensen medium, histology and polymerase chain reaction. RESULTS: A total of 104 patients were included in the study. According to histopathology, 38 patients (36%) were found to have nonspecific chronic lymphadenitis, 27 (26%) had tuberculous lymphadenitis, 11 patients (10.5%) had lymphoma and 9 (8.7%) had histoplasmosis. When Lowenstein-Jensen culture was performed, positive tests for tuberculous lymphadenitis increased by 30%. With polymerase chain reaction, M. tuberculosis DNA was detected in 6 out of 38 samples of non-specific chronic lymphadenitis. Three of these patients were followed up, developed symptoms of tuberculosis and were cured following specific treatment. CONCLUSION: The data obtained in this study suggest that all cases of lymphadenopathies should be submitted to histopathology, Lowenstein-Jensen or Ogawa culture and polymerase chain reaction. Polymerase chain reaction may prove to be useful in providing an early and accurate detection of cases of extrapulmonary tuberculosis in HIV-positive patients with lymphadenopathies, avoiding empirical treatment and the possible development of resistant strains.

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Biologia molecular aplicada às dermatoses tropicais

Cited by 8 publications

References 55 publications

Leprosy: a review of laboratory and therapeutic aspects - Part 2

Leprosy: a review of laboratory and therapeutic aspects - Part 2

Diagnóstico molecular do granuloma lepróide canino, a partir de cortes histológicos emblocados em parafina, pela técnica de reação em cadeia de polimerase (Pcr) – estudo retrospectivo (2002-2009)

HIV-associated tuberculous lymphadenitis: the importance of polymerase chain reaction (PCR) as a complementary tool for the diagnosis of tuberculosis - a study of 104 patients

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