Biological activities of in vitro liverwort Marchantia polymorpha L. extracts

Abstract

<jats:p>To overcome the problems in liverwort collecting such as small size and easily mixed with other species in the wild, we have successfully cultivated Marchantia polymorpha L. under in vitro conditions in the previous study. The aim of this study is to evaluate the biological activities of this in vitro biomass as a confirmation of the sufficient protocol in cultivation this species. Cultured biomass was dried at a temperature of 45-50 oC to constant weight and ground into a fine powder. The coarse powder was extracted with organic solvents of increasing polarization including n-hexane, chloroform, ethyl acetate, and ethanol using the maceration technique. Four extracts were investigated antioxidant (iron reduction power, DPPH), antibacterial (agar diffusion), tyrosinase inhibitory activity, anti-proliferation on MCF-7 cells. Additionally, the presence of natural metabolite groups of the extracts was detected by using specific reagents. For antioxidant activity, ethyl acetate fraction extract had the highest iron reducing power and DPPH free radical scavenging ability with IC50 = 439.31 µg ml-1. All three n-hexane, chloroform, and ethyl acetate extracts possessed resistance to the bacterial strain tested. At a concentration of 2 mg ml-1, n-hexane and chloroform extracts had the highest percentage of tyrosinase inhibition (69.54 and 69.10%, respectively). The n-hexane extract is a potent extract that inhibits the proliferation of MCF-7 cells with the lowest IC50 of 38.15 µg ml-1. A preliminary chemical composition survey showed that the cultured biomass liverwort contains many bioactive compounds, particularly the compounds of range of non- and less-polarized fractions.</jats:p>

Bio-guided isolation of alpha-glucosidase inhibitory compounds from Vietnamese liverwort Marchantia polymorpha: in vitro and in silico studies

“…The solvent ethyl acetate was selected for maceration based on the results reported by Tran et al (2018). 16 These authors prepared four n -hexane, chloroform, ethyl acetate, and ethanol extracts from natural M. polymorpha and evaluated their alpha-glucosidase inhibition. The ethyl acetate extract showed the strongest activity, with an IC 50 value of 10.3 μg mL −1 .…”

“…The ethyl acetate extract showed the strongest activity, with an IC 50 value of 10.3 μg mL −1 . 16 In 2020, this team also evaluated the inhibition of the cultured M. polymorpha biomass, 17 indicating that n -hexane extract showed potent inhibition with an IC 50 value of 11.9 μg mL −1 .…”

“…The extracts of this liverwort showed antioxidant and antibacterial properties, inhibition of tyrosinase and alpha-glucosidase, as well as cytotoxicity against several cancer cell lines. [13][14][15][16][17][18] However, little is known about the alphaglucosidase inhibitors from this medicinal source, with only two reports. 17,18 These authors conducted the biological evaluation of extracts derived from natural and cultured M. polymorpha, indicating the potent inhibition of all prepared extracts.…”

UHPLC–QTOF–MS Metabolic Profiling of Marchantia polymorpha and Evaluation of Its Hepatoprotective Activity Using Paracetamol-Induced Liver Injury in Mice

“…Moreover, a recent study performed on a M. polymorpha L. extract in vitro revealed significant antioxidant and tyrosinase inhibition potential . Besides, endophytes isolated from M.…”

“…11,12 Regarding its biological activity, M. polymorpha exhibited a potent biological potential particularly antifungal, antibacterial, anti-inflammatory, antiviral, and anti-cancer as well that is mainly relied upon its metabolites represented by marchantin A, marchantin B, neomarchantin A, riccardin H, and perrottetin E. 13 Moreover, a recent study performed on a M. polymorpha L. extract in vitro revealed significant antioxidant and tyrosinase inhibition potential. 14 Besides, endophytes isolated from M. polymorpha showed antiviral and anticancer potential owing to their volatile cyclic dipeptides. 15 Tracing the current literature, nothing was found regarding the hepatoprotective potential of M. polymorpha L. as well as its mechanisms of action.…”