Biological activities of pargamicin A, a novel cyclic peptide antibiotic from Amycolatopsis sp.

Abstract

The time-kill studies using pargamicin A against Staphylococcus aureus and Enterococcus faecalis were performed. The effects of the incorporation of radioactive precursors into macromolecules, membrane potential and function using fluorescent dyes were also examined. These studies revealed that rapid bactericidal activity of pargamicin A correlates with the perturbation of bacterial cell membrane potential and membrane function.

Amycolamicin: A Novel Broad‐Spectrum Antibiotic Inhibiting Bacterial Topoisomerase

“…Recently, the relative structure of kibdelomycin, which is structurally closely related to AMM, has been elucidated by NMR analyses in [D 4 ]MeOH. [13] Though the specific rotation [a] 23 D of AMM in methanol is similar to that of kibdelomycin, the 1 H and 13 C NMR spectra of kibdelomycin in [D 4 ]MeOH are not identical with that of AMM as shown in Figures S11 and S12 and Table S1 in the Supporting Information (also see the Supporting Information of Ref. [13]).…”

“…The effects of AMM in this assay were similar to those of novobiocin (NOV), a DNA-synthesis inhibitor. [23] At a dose of 0.3 mm in S. aureus Smith, the biosynthesis of RNA, proteins, the cell wall, and fatty acids were not inhibited. These results strongly indicated that AMM is a DNA-synthesis inhibitor.…”

Structure and antibacterial activities of new cyclic peptide antibiotics, pargamicins B, C and D, from Amycolatopsis sp. ML1-hF4

“…8 Our previous studies revealed that PRG-A exerted rapid bactericidal activity against staphylococci and enterococci via a mechanism that involved disruption of the membrane integrity of the target cells, 9 a mode of action distinct from that of daptomycin. 9,10 During the process of optimization of PRG-A production, we discovered new active components PRG-B, -C and -D (Figure 1) in the culture broth of the PRG-A-producing strain Amycolatopsis sp. ML1-hF4.…”

Tripropeptin C Blocks the Lipid Cycle of Cell Wall Biosynthesis by Complex Formation with Undecaprenyl Pyrophosphate

“…Inhibition of macromolecular synthesis in S. aureus strain Smith by TPPC was assayed by measuring the incorporation of radioactive precursors into the precipitate with 10% trichloroacetic acid for peptidoglycan, DNA, RNA, and protein synthesis as reported previously (3).…”