Biological Activity Alterations of Human Amniotic Membrane Pre and Post Irradiation Tissue Banking

Abstract

Viable HAM was featured with biological activity than fixed HAM prepared by irradiation tissue banking.

Potential Activity of Some Essential Oils and Human Amniotic Membrane against Malassezia furfur and Staphylococcus aureus Isolated from Pityriasis Versicolor Patients

“…This result was also confirmed by Nemr et al (2016) who reported that, gamma irradiation of HAM at a dose of 25kGy affects the quality of the extracted RNA and causes completely degradation of RNA molecules. It is known that intact RNA only presented in metabolic active cells.…”

“…Also, many of clinical trails using amniotic membrane as a biological bandage were published for curing some of skin disorders and ulcers. This is due to several biological benefits revealed by amniotic membrane such as being a nonimmunogenic material, having antimicrobial activity and inducing cellular renewing and growth (Nemr et al, 2016). …”

“…The clear HAM was disinfected by soaking it into 250ml Hanks balanced salt solution (HBSS) contained antibiotic/antimycotic mix into sterilised glass bottle and incubated overnight at 37 o C. For obtaining irradiated HAM, fresh washed transparent HAM was layered on cotton dressing gauzeas (a supporting material), frozen and dried prior to irradiation treatment. (Nemr et al, 2016). The irradiation at dose 25kGy was conducted using Cobalt 60 ( 60 Co) Gamma Cell, product of Canada Co. Ltd. located at NCRRT, Cairo, Egypt.…”

“…HAM cultures were maintained every 3-5 days by adding 5ml fresh medium free from antibiotic/antimycotic, at least two passages were carried out (Nemr et al, 2016).…”

Molecular displaying of differential immunoresponse to various infections of amniotic epithelia

“…Human amniotic epithelial cells (HAECs) were isolated from HAM using a method described previously. 7 About 5×10 6 of HAECs were plated into T-25 cell culture flask that contains 5 mL DMEM: F12 medium supplemented with 10% fetal bovine serum (Lonza, Alpharetta, GA, USA). Cultures were free from further additives such as antibiotics, antimycotics, growth factors, feeder cells, or other stimuli.…”