Biological activity and receptor-binding of ecdysteroids in imaginal discs of Calliphora vicina: a comparison

Terentiou, Penelope; Blattmann, Marianne; Bradbrook, D.A.; Käuser, Gerd; Koolman, Jan

doi:10.1016/0965-1748(93)90091-6

Cited by 16 publications

(12 citation statements)

References 19 publications

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“…This activity profile concurs with earlier in vitro work with a wide variety of insects (Chihara et al, 1972;Mandaron, 1976;Cherbas et al, 1980;Fristrom, 1981;Yund and Osterbur, 1985;Terentiou et al, 1993). Taken together, the current data indicate that the responsiveness of imaginal discs for 20E is similar in multi-resistant and susceptible larvae.…”

Section: Discussionsupporting

confidence: 92%

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Smagghe

Carton

Decombel

et al. 2001

Arch Insect Biochem Physiol

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Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, halofenozide, and RH-5849. To explain differences in toxicity, especially between multi-resistant and laboratory strains, absorption in the body tissues and oxidative metabolism were tested with 14C-labeled ecdysone agonist and a Lineweaver-Burk assay, respectively. Then to address different compound potencies in multi-resistant strains, the potency of the four ecdysone agonists was measured based on their ability to mimic the natural insect molting hormone, 20-hydroxyecdysone (20E) by inducing evagination in isolated imaginal wing discs. Using monoclonal antibody 9B9, the presence of ecdysteroid receptors in imaginal discs in vitro was confirmed. In parallel, Scatchard plot analysis with whole imaginal wing discs cultured with different concentrations of 3H-labeled ponasterone A indicated no significant difference in affinity and in number of target sites for binding between multi-resistant and susceptible laboratory strains. The four compounds tested caused the effect as agonists of 20E in vitro, and typically the order of their toxicities (LC50s) corresponded with that for evagination-induction with whole imaginal discs.

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Section: Discussionsupporting

confidence: 92%

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Smagghe

Carton

Decombel

et al. 2001

Arch Insect Biochem Physiol

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“…This linear relationship (r = 0.994) is rather similar to that for the induction of evagination and the 50% competition by PonA, muristerone A, inokosterone, and 20E in cultured imaginal discs of the blowfly Calliphora vicina (Terentiou et al, 1993) and in wing discs of L. decemlineata and G. mellonella (Smagghe et al, 1996a), as shown in Figure 3. This linear relationship (r = 0.994) is rather similar to that for the induction of evagination and the 50% competition by PonA, muristerone A, inokosterone, and 20E in cultured imaginal discs of the blowfly Calliphora vicina (Terentiou et al, 1993) and in wing discs of L. decemlineata and G. mellonella (Smagghe et al, 1996a), as shown in Figure 3.…”

Section: Discussionsupporting

confidence: 65%

“…In the current assays, we showed that the natural molting hormone 20E or a nonsteroidal dibenzoylhydrazine agonist initiates and sustains the evagination of isolated wing discs. Based on earlier in vitro systems with a wide variety of insects (Cherbas et al, 1980;Oberlander, 1985;Yund and Osterbur, 1985;Terentiou et al, 1993), we found a reliable activity profile of 20E. The premature induction of the wing disc development in vivo caused by tebufenozide and RH-5849 in the last-instar larvae of S. exigua as well as hyperecdysteroid effects have been observed at relatively high doses in separate assays (Smagghe and Degheele, 1994b).…”

Section: Discussionmentioning

confidence: 58%

“…In vitro culture of these tissues may allow direct comparison between larvicidal toxicity and effects on evagination under conditions free of a source of endogenous ecdysteroids (Oberlander, 1985;Terentiou et al, 1993;Smagghe et al, 1996a). We determined in a first series of experiments the toxicity against larval stages of the beet armyworm Spodoptera exigua in vivo and the action on cultured imaginal discs as compared with 20E in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Comparative ecdysteroid action of ring‐substituted dibenzoylhydrazines in Spodoptera exigua

Smagghe

Nakagawa

Carton

et al. 1999

Arch. Insect Biochem. Physiol.

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Ring-substituted dibenzoylhydrazines are well known as nonsteroidal ecdysone agonists that cause precocious larval molt leading to death. Among them, tebufenozide (RH-5992) is used in practice as insecticide to control lepidopteran pests selectively. Recently, a new dibenzoylhydrazine, methoxyfenozide (RH-2485), with a higher activity for Lepidoptera, has been discovered. To obtain insight into the molecular mechanisms involved in the insecticidal selectivity of the dibenzoylhydrazine family, we measured the in vivo toxicity of these dibenzoylhydrazines against larval stages of the beet armyworm, Spodoptera exigua, and their action on in vitro cultured imaginal discs. We found that both nonsteroidal ecdysone agonists induced premature and lethal molting, and caused the same effect as 20-hydroxyecdysone in vitro. Furthermore, we measured the larvicidal activity against S. exigua of a series of dibenzoylhydrazines, in which ring-substituents were varied in a range of halogen, lower alkyl, OCH 3 , SCH 3 , Ph, CN, NO 2 , and CF 3 . The substituent effects on the larvicidal activity against S. exigua were very similar to that for another lepidopteran insect species Chilo suppressalis, suggesting that the molecular mechanism of action of these compounds is similar within both lepidopteran species. Arch. Insect Biochem. Abbreviations used: EcR = ecdysone receptor; EC 50 = evagination of wing discs; IC 50 = hormone binding activity to wing discs; LD 50 = larvicidal toxicity; QSAR = quantitative structure-activity relationship; PonA = ponasteroneA; RH-5992 = tebufenozide; RH-2485 = methoxyfenozide; 20E = 20-hydroxyecdysone.

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“…Early assays to evaluate ecdysteroid activity were based on the displacement of binding of a radioactive ecdysteroid ( 3 H-or 125 I-labeled ponasterone A; PonA) to dipteran and lepidopteran (Cherbas et al 1988 ;Nakagawa et al 2000Nakagawa et al , 2002aNakagawa et al , b, 2005 and coleopteran cell extracts (Harada et al 2011 ) or imaginal disc extracts (Terentiou et al 1993 ;Smagghe and Degheele 1995 ;Smagghe et al 1996 ) . More recently, after the cloning of complete ORFs of EcR and USP, in vitro transcription/translation systems were used to evaluate binding af fi nity and speci fi city for a particular species (Minakuchi et al 2003(Minakuchi et al , 2004(Minakuchi et al , 2007Ogura et al 2005a ;review by Nakagawa 2005 ;Nakagawa and Henrich 2009 ) .…”

Section: Cell-based Screening Assaysmentioning

confidence: 99%

Cell-Based Screening Systems for Insecticides

Smagghe

Swevers

2012

Advanced Technologies for Managing Insect Pests

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Biological activity and receptor-binding of ecdysteroids in imaginal discs of Calliphora vicina: a comparison

Cited by 16 publications

References 19 publications

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Comparative ecdysteroid action of ring‐substituted dibenzoylhydrazines in Spodoptera exigua

Cell-Based Screening Systems for Insecticides

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