Cell-Based Screening Systems for Insecticides

Smagghe, Guy; Swevers, Luc

doi:10.1007/978-94-007-4497-4_6

Cited by 4 publications

(2 citation statements)

References 139 publications

(161 reference statements)

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“…Exposure of AmE‐711 cells to formulations containing different groups of insecticides resulted in a concentration‐dependent loss of cell viability, and also led to atypical changes in cell morphology and up‐regulation of a sHsp gene associated with the cellular stress response. The ability to characterize the response of AmE‐711 cells to different groups of insecticides, both quantitatively and qualitatively, means AmE‐711 could be added as a complement to whole‐organism or colony‐level testing, especially early in the screening process of new insecticides or the re‐evaluation of existing insecticides (Fischer et al, 2019 ; Smagghe & Swevers, 2013 ). Ultimately, AmE‐711 could serve as a research and hazard assessment tool to minimize the risks and maximize the benefits that insecticides offer for honey bees and the environment.…”

Section: Discussionmentioning

confidence: 99%

Using the Honey Bee (Apis mellifera L.) Cell Line AmE‐711 to Evaluate Insecticide Toxicity

Goblirsch

Adamczyk

2022

Enviro Toxic and Chemistry

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One of the main contributors to poor productivity and elevated mortality of honey bee colonies globally is insecticide exposure. Whole-organism and colony-level studies have demonstrated the effects of insecticides on many aspects of honey bee biology and have also shown their interactions with pathogens. However, there is a need for in vitro studies using cell lines to provide greater illumination of the effects of insecticides on honey bee cellular and molecular processes. We used a continuous cell line established from honey bee embryonic tissues (AmE-711) in assays that enabled assessment of cell viability in response to insecticide exposure. We exposed AmE-711 cells to four formulations, each containing a different insecticide. Treatment of cells with the insecticides resulted in a concentration-dependent reduction in viability after a 24-h exposure, whereas long-term exposure (120 h) to sublethal concentrations had limited effects on viability. The 24-h exposure data allowed us to predict the half-maximal lethal concentration (LC50) for each insecticide using a four-parameter logistical model. We then exposed cells for 12 h to the predicted LC50 and observed changes in morphology that would indicate stress and death. Reverse transcription-quantitative polymerase chain reaction analysis corroborated changes in morphology: expression of a cellular stress response gene, 410087a, increased after an 18-h exposure to the predicted LC50. Demonstration of the effects of insecticides through use of AmE-711 provides a foundation for additional research addressing issues specific to honey bee toxicology and complements whole-organism and colony-level approaches. Moreover, advances in the use of AmE-711 in high-throughput screening and in-depth analysis of cell regulatory networks will promote the discovery of novel control agents with decreased negative impacts on honey bees. Environ Toxicol

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Section: Discussionmentioning

confidence: 99%

Using the Honey Bee (Apis mellifera L.) Cell Line AmE‐711 to Evaluate Insecticide Toxicity

Goblirsch

Adamczyk

2022

Enviro Toxic and Chemistry

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“…Insect cell-based expression systems are used for commercial production of various veterinary and human vaccines 1,2 , and screening systems with insect cells are exploited for developing new insecticides 3 . The availability of such expression systems relies on the development of genetic engineering tools, but the development is often limited to specific cell lines.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines

Miyata

Tokumoto

Sogame

et al. 2019

Sci Rep

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Larvae of the African midge Polypedilum vanderplanki (Diptera: Chironomidae) show a form of extreme desiccation tolerance known as anhydrobiosis. The cell line Pv11 was recently established from the species, and these cells can also survive under desiccated conditions, and proliferate normally after rehydration. Here we report the identification of a new promoter, 121, which has strong constitutive transcriptional activity in Pv11 cells and promotes effective expression of exogenous genes. Using a luciferase reporter assay, this strong transcriptional activity was shown to be conserved in cell lines from various insect species, including S2 ( Drosophila melanogaster , Diptera), SaPe-4 ( Sarcophaga peregrina , Diptera), Sf9 ( Spodoptera frugiperda , Lepidoptera) and Tc81 ( Tribolium castaneum , Coleoptera) cells. In conjunction with an appropriate selection maker gene, the 121 promoter was able to confer zeocin resistance on SaPe-4 cells and allowed the establishment of stable SaPe-4 cell lines expressing the fluorescent protein AcGFP1; this is the first report of heterologous gene expression in this cell line. These results show the 121 promoter to be a versatile tool for exogenous gene expression in a wide range of insect cell lines, particularly useful to those from non-model insect species.

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G-Protein-Coupled Receptors (GPCRs) as Biopesticide Targets: A Focus on Octopamine and Tyramine Receptors

Gross

Kimber

Coats

2014

ACS Symposium Series

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Cell-Based Screening Systems for Insecticides

Cited by 4 publications

References 139 publications

Using the Honey Bee (Apis mellifera L.) Cell Line AmE‐711 to Evaluate Insecticide Toxicity

Using the Honey Bee (Apis mellifera L.) Cell Line AmE‐711 to Evaluate Insecticide Toxicity

Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines

G-Protein-Coupled Receptors (GPCRs) as Biopesticide Targets: A Focus on Octopamine and Tyramine Receptors

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