Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Abstract

Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress and from various chronic diseases. Papaver rhoeas has a long history of medicinal usage, especially for ailments in adults and children. The possible cytotoxicity, genotoxicity and potential antioxidant effect of plant extract isolated from flowers of Papaver rhoeas was investigated in human lymfoblastoid cell line (TK6). Antioxidant activity of this extract was determined using the DPPH assay. The plant extract exhibited dose dependent free radical scavenging ability. The growth activity assay was used for determination of cytotoxicity. To assess potential genotoxicity the comet assay was used. The lower extract concentrations (0.25 and 0.5 mg/ml) neither exerted cytotoxic, nor genotoxic effects in TK6 cells but they stimulated cell proliferation. The concentration 25 mg/ml scavenged almost 85% of DPPH free radical. On the other hand, this concentration had strong cytotoxic and genotoxic effect on TK6 cells.The balance between beneficial and harmful effects should be always considered when choosing the effective dose. Plants are universally recognized as a vital part of the world's natural heritage and up to 80% of the population relies on plants for their contribution to a primary healthcare. The use of plants in traditional medicine is widespread. Many plants still represent a large source of natural antioxidants that might serve as leads for the development of novel drugs. It is commonly accepted that due to oxidative stress, reactive oxygen species (ROS) such as superoxid, hydroxyl and peroxyl radicals are generated [1]. Recently much attention has been given to the natural phytochemicals with antioxidant, antimutagenic and anticarcinogenic activities [2][3][4][5][6][7][8]. These compounds are of great importance for chemoprevention and potential cancer biotherapy.Papaveraceae is a family of flowering plants. The flowers of poppy are chiefly employed as a mild pain reliever and as a treatment for irritable coughs; it also helps to reduce nervous over-activity [9]. The flowers and petals are anodyne, expectorant, hypnotic, slightly narcotic and sedative. An infusion is taken internally in the treatment of bronchial complaints and coughs, insomnia, poor digestion, nervous digestive disorders and minor painful conditions. The flowers are also used in the treatment of jaundice and -in very small quantities-as a sleep-inducing drug [9,10].Papaver rhoeas plant extract contains a lot of flavonoids: kaempferol, quercetin, luteolin and hypolaetin andAntioxidant activity of the kaempferol [12][13], quercetin [14][15] and luteolin [16] was already demonstrated. Total phenolic compounds (9.73 -19.91 mg GAE/g) and total flavonoids (7.904-11.45 mg QE/g) were determined in fresh petals [17].Besides flavonoids and their glycosides Papaveraceae family are known for the presence of alkaloids as morphine, codeine, narcotine, thebaine and papaverine [8,18,19].The aim of this study...

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

“…We have also modified experimental conditions under which the adaptive response in this test system could be detected. Results obtained in this and our previous studies [12,30,43] confirm important role of plants and plant extracts in prevention against various negative impacts and thus can be potentially used in pharmacology and medicine.…”

“…Recently much attention has been given to the natural phytochemicals with antioxidant, antimutagenic and anticarcinogenic activities [6][7][8][9][10][11]. These compounds are of a great importance for chemoprevention and potential cancer biotherapy [12].…”

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

“…Antioxidant supplements or food, vegetables, and plants containing antioxidants can be used to reduce oxidative damage induced by reactive oxygen/ nitrogen species (ROS/RNS) [65]. Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress as well as from various chronic diseases [66]. Papaver bracteatum, is also known as the Iranian poppy [67].…”

“…The results of poppy ethanolic extract indicated the presence of compounds which are able to scavenge free radicals. The free-radical scavenging activity [68] and antioxidant activity [66] of this extract was determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay described previously. The molecules possessing radical-scavenging properties present in ethanolic poppy extract can quench DPPH free radicals [68].…”

Phytochemical and biological research of Papaver pharmaceutical resources

“…The possible cytotoxicity, genotoxicity, and antioxidant effect of extract from flowers of P. rhoeas were investigated in human lymphoblastoid cell line (TK6) (Hasplova et al, 2011). Antioxidant activity was determined using the DPPH assay.…”