Biological activity of sponge-associated fungi from Karimunjawa Islands, Indonesia against pathogenic Streptococcus pneumoniae

Cristianawati, Olvi; Sabdaningsih, Aninditia; Becking, Leontine E.; Khoeri, Miftahuddin Majid; Nuryadi, Handung; Sabdono, Agus; Trianto, Agus; Radjasa, Ocky Karna

doi:10.13057/biodiv/d200807

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…Curvularia lunata, a fungus, was isolated from the sponge Cinachyrella australiensis from the Karimunjawa Islands in Indonesia. C. lunata fungal extract demonstrated promising antibacterial activity against MDR S. pneumoniae [72].…”

Section: Antimicrobial Compoundsmentioning

confidence: 99%

Bioactive Novel Natural Products from Marine Sponge: Associated Fungi

Venkataraman¹,

Vaithi²,

Singaram³

2022

Fungal Reproduction and Growth

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Marine sponges are distributed in the water, from the intertidal zones to thousands of meters deep. They are primitive multicellular invertebrates that live in benthic environments and are bound to solid substrates. Filter feeders, sponges have many microscopic pores on their surface, which allow water to enter and circulate via a network of canals where microbes and organic particles are filtered out and absorbed. Marine fungi are widespread in the oceans and colonize different ecological niches; they are found associated with organisms of all trophic levels and can act as saprobes, symbionts, and parasites. Compared with other marine microorganisms, marine fungus is relatively understudied. Fungi associated with sponges have been discovered to be a promising source of pharmacologically active compounds with unique anticancer, antibacterial, and antiviral properties.

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Section: Antimicrobial Compoundsmentioning

confidence: 99%

Bioactive Novel Natural Products from Marine Sponge: Associated Fungi

Venkataraman¹,

Vaithi²,

Singaram³

2022

Fungal Reproduction and Growth

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“…The tapping method was used for the fungal isolation by tapping gastropod tissue on malt extract agar (MEA) media (MERK, USA) with three repetitions. Isolation media was supplemented with 100 mg.L -1 of chloramphenicol to avoid bacterial contamination (Cristianawati et al, 2019). The petri dishes contain the samples were incubated at room temperature for seven days.…”

Section: Gastropods Collection and Fungi Isolationmentioning

confidence: 99%

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

Trianto,

Radjasa,

Subagiyo

et al. 2023

Indo. J. Mar. Sci.

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Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes. Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia. The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio were indicated by clear zones formed around the isolates. A total of 13 fungal species were isolated from C. cingulata (19MB-C5 and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18). Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%). A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.

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“…The Vibrio bacteria were grown on nutrient broth to a concentration of 0.5 McFarland and then inoculate on a trypticase soy agar (TSA) plate (Merck, Germany) using sterile cotton swabs (ONEMED, Indonesia). Seven days old of the fungal disk was plugged on TSA and incubated at 27ºC for 24h (Sibero et al 2018;Cristianawati et al 2019).…”

Section: The Anti-vibrio Screeningmentioning

confidence: 99%

Potential of marine sponge-derived fungi in the aquaculture system

2021

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Abstract. Bahry MS, Radjasa OK, Trianto A. 2021. Potential of marine sponge-derived fungi in the aquaculture system. Biodiversitas 22: 2883-2892. Organic waste from aquaculture is one of the triggers of disease outbreaks and a decrease in water quality that urgently needs to be resolved. Indonesia has a high diversity of sponges including their associated microorganisms that potential in the field of biotechnology. This study aimed to determine the enzymatic and anti-vibrio activity of fungi associated with marine sponges and identify potential fungi. The specimen of sponges was collected from Samalona Island, South Sulawesi, Indonesia. The enzymatic and anti-vibrio assay was conducted by using the plug method and the activity was determined by a clear zone around the fungal isolates. Fungal identification was carried out molecularly using universal primers ITS1 and ITS4 and phylogenetic tree analysis. The fungal isolates were screened for the extracellular enzyme activity (amylase, cellulase, protease) and anti-vibrio activity against Vibrio parahaemolyticus, V. harveyi, and V. vulnificus). A total of three fungal isolates have been isolated from the sponge Monanchora sp. Isolate SL 3 SP 3.3 had potential enzymatic activities with Enzymatic Indeks (EI) 3.95±0.17 on amylase, 3.75±0.36 on cellulase, 5.38±0.30 on protease. The highest anti-vibrio activity was obtained against V. harveyi with an inhibition zone diameter of 4.82 ±0.37 mm. The results of fungal identification showed that isolate SL3SP3.3 had a sequence length of 638 bp and was closely related to Trichoderma reesei a.k.a Hypocrea jecorina with a similarity value of 99.69%.

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Biological activity of sponge-associated fungi from Karimunjawa Islands, Indonesia against pathogenic Streptococcus pneumoniae

Cited by 5 publications

References 46 publications

Bioactive Novel Natural Products from Marine Sponge: Associated Fungi

Bioactive Novel Natural Products from Marine Sponge: Associated Fungi

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

Potential of marine sponge-derived fungi in the aquaculture system

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