Biological activity ofTreponema hyodysenteriae hemolysin

Saheb, Samir A.; Lallier, R.; Massicotte, Luc; Lafleur, L.; Lemieux, Suzanne

doi:10.1007/bf01567426

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…Photomicrographs reveal that these lesions are populated by large numbers of T. hyodysenteriae cells, frequently in the midst of swine erythrocytes (1,13). T. hyodysenteriae cells produce a hemolysin (24) which releases erythrocyte cytoplasmic contents into their environment.…”

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Erythrocytes as a source of essential lipids for Treponema hyodysenteriae

Stanton¹,

Cornell²

1987

Infect Immun

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Mammalian erythrocytes were tested as a nutrient source for the spirochete Treponema hyodysenteriae, the etiologic agent of swine dysentery. Brain heart infusion (BHI) broth did not support growth of T. hyodysenteriae B204 or B78. However, BHI broth supplemented with washed bovine erythrocytes, erythrocyte membranes, or cholesterol was an excellent culture medium for these strains (4 x 108 to 109 cells per ml, final population densities). Small amounts of cholesterol (3 to 10 ,ug/ml of medium) were required for maximum cell yields of strain B204. Of various sterols and sterol-like compounds tested, cholestanol and sitosterol could substitute for cholesterol. BHI-dehydrated medium extracted with CHCl3-CH30H (2:1, vol/vol) to remove lipids was used to prepare lipid-depleted culture broth. Lipid-depleted broth supplemented only with cholesterol did not support T. hyodysenteriae B204 growth. However, this same broth supplemented either with vesicles made from cholesterol and phosphatidylcholine or with washed erythrocytes was a good culture medium for the spirochete. Thus, both cholesterol and a phospholipid are needed for T. hyodysenteriae growth in lipid-depleted broth. Mammalian erythrocytes can be used by the spirochete as a source of these lipids.

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Erythrocytes as a source of essential lipids for Treponema hyodysenteriae

Stanton¹,

Cornell²

1987

Infect Immun

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“…One of the putative virulence factors of S. hyodysenteriae is the production of a beta-hemolysin (46,47,50,52,59,(70)(71)(72)(73). Picard et al (68) initially described the increase of beta-hemolysin production by spirochetes grown in broth supplemented with sodium ribonucleate.…”

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confidence: 99%

The role of tumor necrosis factor and secondary bacteria in the pathogenesis of swine dysentery

Nibbelink

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“…Infections with Treponema hyodysenteriae have been shown to induce a severe mucohemorrhagic diarrheal disease of pigs (8,11,12). Virulence has been attributed to the presence of a beta-hemolysin (20,29,30), a biologically active endotoxin (25,26), and a potential cytotoxin (M.…”

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Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum

Nibbelink

Wannemuehler

1990

Infect Immun

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The pathogenic mechanisms responsible for the development of lesions in swine and mice after infection with Treponema hyodysenteriae have not been fully characterized. The release of inflammatory mediators from mast cells has been postulated to play a role in lesion development during swine dysentery. Therefore, C3H/HeN mice were infected with T. hyodysenteriae, and mucosal mast cell (MMC) numbers were examined in cecal sections. An initial increase in MMC numbers from 13 to 22 per 50 crypt villus units was observed, but at 20 days postinfection the numbers significantly decreased (P < 0.05) to 5 MMC per 50 crypt villus units. Immunohistochemical analysis performed on cecal sections failed to show a significant change in lamina proprial T-lymphocyte subsets. Numbers of T. hyodysenteriae CFU recovered from the cecum were stable throughout the experimental time period. Mast cell-deficient W/WV mice and their mast cell-sufficient littermates were also infected to determine whether MMCs were necessary for the occurrence of T. hyodysenteriae-induced lesions. W/W' mice were as susceptible to infection as their normal littermates and developed similar macroscopic and microscopic lesions. These results indicate that changes in MMC numbers can be detected after an infection with T. hyodysenteriae; however, on the basis of observations of infected W/Wv mice, mast cells are not required for lesion development in the murine model.

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Biological activity ofTreponema hyodysenteriae hemolysin

Cited by 9 publications

References 10 publications

Erythrocytes as a source of essential lipids for Treponema hyodysenteriae

Erythrocytes as a source of essential lipids for Treponema hyodysenteriae

The role of tumor necrosis factor and secondary bacteria in the pathogenesis of swine dysentery

Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum

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