Biological and Chromatographic Characterization of a Polypeptide with Pressor and Oxytocic Activities Isolated from Bovine Pineal Gland

Milcu, Ștefan Marius; Pavel, S.; Neacsu, C

doi:10.1210/endo-72-4-563

Cited by 93 publications

(20 citation statements)

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“…With the specific and sensitive RIAs developed in Amsterdam no immunoreactivity for AVT, AVP or OT could be detected in this fraction. Th~is confirms the findings of Milcu et al (1963) that the vasopressor and oxytocic activities observed in this fraction were not due to AVP or OT. It also confirms the conclusion of Neacsu (1972) that AVT was not present in the fraction.…”

Section: Resultssupporting

confidence: 89%

The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

Pévet

Neacsu²,

Holder

et al. 1981

J. Neural Transmission

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Section: Resultssupporting

confidence: 89%

The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

Pévet

Neacsu²,

Holder

et al. 1981

J. Neural Transmission

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“…A second reason for evaluating the effects of vasotocin was the recent discovery (5,6) that this hormone, which is absent from the mammalian neurohypophysis, is present in the mammalian pineal body (cow and pig). Numerous studies (7) have suggested a relationship between the pineal body, salt balance in general, and aldosterone secretion in particular.…”

Section: Effect Of Right Renal Intraarterial Infusion Of Arginine Vasmentioning

confidence: 99%

Inhibition of Renin Release in the Dog by Vasopressin and Vasotocin

Vander

1968

Circulation Research

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In anesthetized dogs the intravenous infusion of arginine vasotocin, 25 mU/min, or vasopressin, 10 mU/min, inhibited renin secretion when control secretion rates were normal or when they were elevated by complete ureteral occlusion. Vasotocin, 5 mU/min, or vasopressin, 1 mU/min, was ineffective. Direct intraarterial infusion of vasotocin, 5 mU/min, into the right kidney inhibited renin secretion by the right kidney only. Glomerular filtration rate and renal plasma flow were not changed, and mean arterial pressure was either not changed or was decreased. Vasotocin caused no change in urine flow but did produce a significant natriuresis secondary to decreased tubular reabsorption. The physiological implications of these polypeptide-renal relationships are discussed.ADDITIONAL KEY WORDS posterior pituitary polypeptides sodium balance pineal arginine vasotocin angiotensin renal tubular sodium reabsorption• Bunag, Page, and McCubbin (1) have reported that vasopressin can inhibit renin release. The present studies were performed to test the effects of arginine vasotocin and to extend their observations on vasopressin. Methods Animal Techniques and ProtocolsExperiments were performed on dogs maintained on standard laboratory chow (Friskies) and anesthetized with pentobarbital, 30 mg/kg iv. The right and left ureters were catheterized via a right flank incision. To obtain renal venous blood, a polyethylene catheter (2.4 mm, o.d.) was introduced into the left femoral vein, passed up the inferior vena cava and manipulated into the right renal vein. In three dogs, a catheter was passed from a jugular vein through the vena cavae and right atrium into the left renal vein. In the same dogs, a 24-gauge needle was introduced into the renal artery close to its origin and was left in place throughout the experiment, the needle being constantly perfused via a catheter with isotonic saline, 0.4 ml/min.From the Department of Physiology, University of Michigan, Ann Arbor, Michigan 48104.This investigation was supported in part by U. S. Public Health Service Research Grant AM 05077 from the National Institutes of Health.The author is a John and Mary R. Markle Scholar in Academic Medicine.Accepted for publication September 14, 1968.Arterial blood was obtained from a femoral artery catheter. Arterial blood pressure was monitored continuously using a Statham gauge and Grass polygraph. Renal excretory and hemodynamic data were obtained by the clearance technique; periods were 10 minutes long with arterial and renal venous blood samples taken in the middle of each period. Creatinine clearance was used as a measure of glomerular filtration rate; total renal plasma flow was determined by the Fick principle, using PAH. In experiments employing complete ureteral occlusion, renal blood flow was measured with a Carolina squarewave electromagnetic flowmeter (model 301) and probe. Experimental observations were not begun until at least 45 minutes after completion of all experimental protocols and administration of priming doses of creatini...

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“…Whereas central effects of OT involve induction of specific reproductive behaviors, peripheral effects include uterine contractions, milk ejection, natriuresis and vasodilation (Zingg 1996, Thibonnier et al 1999. Centrally, the OT gene is expressed in specific hypothalamic neuroendocrine cells as well as in the pineal gland (Milcu et al 1963, Fisher & Fernstrom 1981, Liu & Burbach 1987. Peripherally, the gene is expressed in uterine epithelium (Lefebvre et al 1992), fetal membranes (Mitchell et al 1998) and the corpus luteum (Ivell et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Activation of the mouse oxytocin promoter by the orphan receptor RORalpha

Chu

Zingg

1999

Journal of Molecular Endocrinology

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Although an increasing number of nuclear orphan receptors have recently been identified, the number of known naturally occurring genes that are directly regulated by orphan receptors is still small. We have shown previously that the gene encoding the neuropeptide oxytocin (OT) is negatively regulated by the orphan receptors chicken ovalbumin upstream transcription factor I (COUP-TFI) and II. Here we show that the mouse OT gene promoter is activated by ROR , a representative of the ROR/RZR orphan receptor subfamily. Using promoter/chloramphenicol acetyltransferase reporter constructs in heterologous transfection assays, we determined that ROR action induces a <6-fold increase in promoter activity. By 5 and 3 deletion analysis, DNase footprint analysis and electrophoretic mobility shift assays, we found that ROR action is mediated by two 14 bp regions centered at 160 and 180 nucleotides upstream of the transcriptional initiation site. Both sites contain significant sequence identities with an established ROR recognition sequence. Mutations in either or both of these sites reduce significantly RORinduced activation of the OT promoter. In view of the strong transcriptional activation exerted by ROR on the OT gene promoter and the widespread distribution of different members of the ROR/RZR family, interactions between ROR/RZR isoforms and the OT gene may form part of the multifactorial regulatory mechanisms that control OT gene expression in different tissues.

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Biological and Chromatographic Characterization of a Polypeptide with Pressor and Oxytocic Activities Isolated from Bovine Pineal Gland

Cited by 93 publications

References 0 publications

The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

Inhibition of Renin Release in the Dog by Vasopressin and Vasotocin

Activation of the mouse oxytocin promoter by the orphan receptor RORalpha

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